Wyniki wyszukiwania - Biblioteka Nauki

1

Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

100%

Stojecki K. , Sroka J. , Karamon J. , Kusyk P. , Cencek T.

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nr 1

2

Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

100%

Stojecki K. , Sroka J. , Karamon J. , Kusyk P. , Cencek T.

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nr 1

EN

Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and the effect of each component of pre-treatment solutions on PCR reactions. Seven stool concentration techniques were compared. The results showed that the most efficient concentration method for stool sample preparation for detection of G. intestinalis by PCR is centrifugal flotation with Percoll (with saturated NaNO₃ as the flotation solution). This method is relatively inexpensive, less labor-intensive, and suitable for epidemiological monitoring and clinical investigations.

3

Forkhead transcription factor complex with special focus on Foxp3, Foxn1, and Foxj1 genes determining immunological parameters - a review

86%

Wojtaszczyk P. , Kostro K. , Niemczuk K. , Lisiecka U. , Stojecki K. , Zmuda A.

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nr 4

EN

The Forkhead genes (transcription complex Fox) play many important roles in the maintenance and determination of biological processes underlying carbon-based life. Hie expression of Fox genes occurring as a result of reciprocal interactions at the transcriptional level influences the correct function of the immune system as regards the context of general activity of immunological parameters, as well as exposure to aetiological agents. In the case of model organisms and species, in which the knowledge about genomic sequences is incomplete, understanding the above-mentioned transcriptional complex is still insufficient, despite the existence of numerous scientific publications. It is worth noting that Fox genes exist in amphioxus Branchiostoma lanceolatum and in the majority of species they were not characterised. Among many coding sequences included in the transcriptional complex Forkhead, Foxp3, Foxn1, and Foxj1 genes are of a great importance from immunological point of view, being partially jointly responsible for determining features related to productivity. The paper provides general characterisation of selected Fox genes and is an introduction to the presentation of new data, which can be applied at the level of biological differentiation of the populations of domestic and wild animals.

4

Selected parameters of immune response in pregnant sows vaccinated against Trueperella pyogenes

86%

Kostro K. , Lisiecka U. , Zmuda A. , Niemczuk K. , Stojecki K. , Taszkun I.

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tom 58

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nr 1

EN

Expression of CD4, CD8, and CD25 surface markers on T lymphocytes and levels of IFNγ, IL-10, and TNF-α in colostrum and milk were determined in sows vaccinated against Trueperella pyogenes in the final stage of pregnancy. The autovaccine, prepared from Trueperella pyogenes, administered twice to pregnant sows six and three weeks before the anticipated delivery significantly increased the percentages of TCD4+, TCD8+, and TCD25+ as well as levels of IFNγ, TNF-α, and IL-10 in colostrum and milk. The enhanced immune potential of colostrum effectively protected the piglets against T. pyogenes infections during weaning and thus reduced the economic losses on the particular farm concerned, where T. pyogenes infections occur endemically. Knowledge of the profile of cellular and humoral immune response in colostrum and milk of vaccinated sows will enable the design of a T. pyogenes infection prophylactic programme for suckling pigs and weaners, which are most susceptible to infections.

5

Assessment of viability of the nematode eggs (Ascaris, Toxocara, Trichuris) in sewage sludge with the use of LIVE/DEAD Bacterial Viability Kit

86%

Dabrowska J. , Zdybel J. , Karamon J. , Kochanowski M. , Stojecki K. , Cencek T. , Klapec T.

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nr 1

6

Potential role of beavers (Castor fiber) in contamination of water in the Masurian Lake District (north-eastern Poland) with protozoan parasites Cryptosporidium spp. and Giardia duodenalis

58%

Sroka J. , Gizejewski Z. , Wojcik-Fatla A. , Stojecki K. , Bilska-Zajac E. , Dutkiewicz J. , Cencek T. , Karamon J. , Zajac V. , Kusyk P. , Dabrowska J. , Kochanowski M.

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nr 2

EN

The purpose of this study was to assess the possible influence of beavers on the contamination of lake water with zoonotic parasites Giardia duodenalis and Cryptosporidium spp., with respect to the risk to human health. A total of 79 water samples were taken around the habitats of beavers from 14 localities situated in the recreational Masurian Lake District (north-eastern Poland). Water was sampled in the spring and autumn seasons, at different distances from beavers' lodges (0-2, 10, 30, and 50 m). The samples were examined for the presence of (oo)cysts of zoonotic protozoa Giardia duodenalis and Cryptosporidium spp. by direct fluorescence assay (DFA) and by nested and real time PCR. By DFA, the presence of Giardia cysts was found in 36 samples (45.6%) and the presence of Cryptosporidium oocysts in 26 samples (32.9%). Numbers of Giardia cysts, Cryptosporidium oocysts, and summarised (oo)cysts of both parasites showed a significant variation depending on locality. The numbers of Giardia cysts significantly decreased with the distance from beavers' lodges while the numbers of Cryptosporidium oocy sts did not show such dependence. The amount of Giardia cysts in samples collected in spring was approximately 3 tiir.es higher than in autumn. Conversely , a larger number of Cryptosporidium oocy sts were detected in samples collected in autum than in spring. By PCR, Giardia DNA was found in 38 samples (48.1%) whereas DNA of Cryptosporidium was found in on samples (8.9%). Eleven Giardia isolates were subjected to phylogenetic analy sis by restriction fragment length poly morphism PCR or sequencing which evidenced their belonging to zoonotic assemblages: A (3 isolates) and B (8 isolates). In conclusion, water in the vicinity of beavers' lodges in the tested region was markedly contaminated with (oo)cysts of Giardia duodenalis and Cryptosporidium spp., which confirms the potential role of beavers as a reservoir of these parasites and indicates a need for implementation of appropriate preventive measures to protect tourists' health.

7

Molecular diagnostics of Sarcocystis spp. infections

50%

Stojecki K. , Karamon J. , Sroka J. , Cencek T.

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