Canine Monocytic Ehrlichiosis is caused by pathogenic rickettsia Ehrlichia canis and is a tick-borne disease transmitted in particular by Rhipicephalus sanguineus and Dermacentor variabilis. The role of other ticks in the CME transmission is still unrecognised. The aim of this screening study on a tick population (n=490) was to determine the possibility of transmitting Ehrlichia canis infection by ticks during dog’s infestation as well as examining the possibility of co-infection with Ehrlichia canis, Borelia burgdorferi and Anaplasma phagocytophila. Examinations were performed with the use of nested polymerase chain reaction (PCR). There was no positive reaction to E. canis on 490 DNA isolates of ticks and no co-infection with Ehrlichia canis, Borelia burgdorferi and Anaplasma phagocytophila was found in the examined tick population. Taking under consideration the negative results in tick populations from the epizootiological point of view, the route of dog infection seems to be problematic despite the fact that there were positive samples in the indirect fluorescent antibody test (IFAT) and in PCR.
Szczepy H. influenzae wytwarzające otoczkę polisacharydową, odmiennie do szczepów bezotoczkowych stanowią zwartą klonalnie grupę. Celem pracy było określenie poziomu zmienności szczepów H. influenzae typu b izolowanych od zdrowych dzieci z przedszkoli oraz domów dziecka oraz szczepów izolowanych z zakażeń inwazyjnych. W pracy oceniono przydatność metody PFGE w badaniach transmisji szczepów Hib krążących w populacji oraz dochodzeniach epidemiologicznych w obrębie trzech wykrytych ognisk zakażenia inwazyjnego szczepami Hib.
EN
Goal of the work was to evaluate the differences level among H. influenzae strains of b serotype isolated from children attending day-care centres and orphanages and among strains isolated from invasive infections. In the work PFGE in Hib strains transmission examination and for epidemiological studies among three sources of invasive infection was applied. Among 35 Hib strains tested and control strain 8 different pulsotyped were found. Among 21 strains colonising the nasopharynx of healthy children, and among 13 Hib strains isolated from cerebrospinal fluid, 6 and 1 pulsotypes were fund, respectively. Results obtained show that healthy children might be colonizing with genotypes characteristic for Hib strains isolated from invasive infections. In this view wider Hib vaccination seems be expected, as Hib circulation is common.
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