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1

Elimination of aflatoxin B1 in broiler chicks by clinoptilolite

100%

Skalicka M. , Makoova Z.

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nr 2

EN

During 19-day study with broiler poultry, broiler chicks were fed diet containing clinoptilolite as supplement at the concentrations of 3% and 5%. At the same time, the following parameters were observed: activity of alkaline phosphatase (ALP) and body weight of broiler chicks. ALP activity in broiler chicks (groups G4, G5, and G6) fed aflatoxin B₁ was enhanced during all days of the observation in comparison with the control broiler chicks G1. Significant increase in ALP (P ≤ 0.05) was recorded from day 42 of the age onwards in group G4 of broiler chicks in comparison with the control group G1. Significant increase in ALP (P ≤ 0.01) was recorded from day 42 of the age onwards in group G5 of broiler chicks in comparison with the broiler chicks in group G2 and significant increase in ALP (P ≤ 0.01) was recorded onwards in group G6 of broiler chicks in comparison with the broiler chicks in group G3. Significant increase in ALP (P ≤ 0.05) was recorded from day 49 of the age onwards in group G5 of broiler chicks in comparison with the broiler chicks in group G2 and in group G6 of broiler chicks in comparison with the broiler chicks in group G3. The body weight of broiler chicks from group G2 (supplementation with 3% clinoptilolite) was 229.33 g higher in comparison with the control group G1. In group G3 fed feed mixture supplement with 5% concentration of clinoptilolite, the final mean body weight was only 179.33 g higher in comparison with the control group G1. The results of our study suggest that clinoptilolite as mineral non-nutritional supplement in feed mixture may protect from the effect of aflatoxin B₁ in young growing broilers. The supplementation of poultry diet with 3% clinoptilolite proved to be a suitable method to reduce the risk of the adverse effects of aflatoxin B₁ on poultry organism.

2

Influence of Natrium Humate on Sorption of Cadmium and Zinc Levels in Poultry

80%

Korenekova B. , Skalicka M. , Nad P.

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2003

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tom Vol. 10, nr 6

553-557

EN

Experiment was conducted with broiler chickens to examine the effect of natrium humate (HuNa) on zinc (Zn) and cadmium (Cd) concetrations after cadmium addition. Poultry were divided into 3 groups: G1) control group; G2) 3.0 [formula]; and G3) 1% HuNa feed mixture with 3.0 [formula]. The samples of liver and muscles of poultry were analysed for the presence of Cd and Zn. Zn concentrations in liver were generally lower in groups (G2, G3) in comparison with control group (G1). Mean Zn levels (g2) in muscles [formula] and liver [formula] were decreased in comparison with G1 [formula], respectively. Addition of HuNa into the feed mixture of broilers (G3) had increased Zn levels in liver (from 16.57 to 18.25 [formula]) and significantly increased (p=0.05) in muscles (from 1.97 to 2.08 [formula]) in comparison with group (G2), with addition of Cd. Concentrations of Cd decreased in muscles (from 0.47 to 0.36 [formula]) and liver (from 1.93 to 1.62 [formula]) in comparison with group G2.

PL

Eksperyment przeprowadzono na brojlerach w celu zbadania wpływu dodania huminianu sodu (HuNa) do paszy na stężenie kadmu i cynku w ich ciele pod wpływem dawki kadmu. Drób podzielono na 3 grupy: G1 - grupa kontrolna, G2 - 3,0 [wzór]; G3 - 1% HuNa do paszy i 3,0 [wzór]. Badano próbki wątroby i mięśni drobiu na obecność Cd i Zn korzystając ze spektrometru absorpcji atomowej (Unicam Solar 939). Stężenia Zn w wątrobie z reguły były mniejsze w grupach: G2 i G3 w porównaniu z grupą kontrolną (G1). Średni poziom Zn (G2) w mięśniach (1,97 mg/kg) i wątrobie (16,57 mg/kg) zmniejszał się w porównaniu z wartością dla G1 (odpowiednio 5,35 i 21,34 mg/kg). Dodanie HuNa do karmy brojlerów (G3) spowodowało podwyższenie się poziomu Zn w wątrobie (z 16,57 do 18,25 mg/kg) i znaczny wzrost (p=0,05) w mięśniach (z 1,97 do 2,08 mg/kg) w porównaniu do grupy G2, gdzie dodawano kadm. Stężenie kadmu malało w mięśniach (z 0,47 do 0,36 mg.kg) i w wątrobie (z 1,93 na 1,62 mg/kg) w porównaniu z grupą G2.

3

Comparison of heavy metal concentrations in the organs of yellow-necked mouse (Apodemus flavicollis, Melchior, 1834) from areas of nuclear power station and power station

61%

Jančova A. , Massanyi P. , Nad P. , Korenekova B. , Skalicka M. , Drabekova J.

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tom Vol. 12, nr 11

1221-1227

EN

The aim of this work was to determine concentration and distribution of copper, iron and cadmium in organs of Apodemus flavicollis (Melchior, 1834) in relation to the sex and locality occurrence. The females of Apodemus flavicollis from the surroundings of the nuclear power station Mochovce show significantly higher concentration of copper in the liver (p < 0.05) in comparison with males. In females a statistically (p < 0.05) higher levels of copper in the liver (5.005 mg . kg[to -1]) and uterus (0.0077 mg . kg[to -1]) were detected. The highest concentrations of iron were found in uterus (471.733 mg . kg[to -1]). In all animals there is significantly higher (p < 0.05) concentration of cadmium in kidneys in comparison with liver, testis and uterus. The highest concentrations of copper in the organs of Apodemus flavicollis from the surroundings of the rower station Novaky were found in uterus (10.264 mg . kg[to -1]). In males the liver was the most contaminated organ (7.565 mg . kg[to -1]). The highest iron concentration was found in uterus (618.5 mg . kg[to -1]). Concentration of iron in liver (445.0 mg . kg[to -1]) in males was significantly higher (p < 0.05) in comparison with testis. In male kidneys there was a statistically higher amount of iron (p < 0.05) than in the testis. In the kidneys of the females there was a significantly higher level of iron when compared with males (p < 0.01). The concentration of cadmium in the kidneys of females is significantly higher (p < 0.01) than concentration in the liver and in uterus. Significant difference (p < 0.05) was also found in liver in comparison with uterus. The females of Apodemus flavicollis from Novaky locality have shown significantly higher concentration of copper in uterus (p < 0.05) and in kidneys (p < 0.01), higher concentration of iron in kidneys (p < 0.01) and higher concentration of cadmium in kidneys (p < 0.01) and liver (p < 0.01) in comparison with females from Mochovce locality.

PL

Zawartość miedzi w wątrobie samic myszy leśnej Apodemus flavicol/is (Melchior, 1834) z rejonu elektrowni atomowej w Mochowcach była większa niż w wątrobie samców (p < 0.05). Zawartość miedzi była większa w wątrobie niż macicy. Największa zawartość żelaza została stwierdzona w macicy. Poziom kadmu był relatywnie mały we wszystkich badanych próbkach. Nie stwierdzono żadnych różnic zawartości kadmu pomiędzy płciami. U wszystkich zwierząt zawartość kadmu jest znacznie większa w nerkach niż w wątrobie, jądrach i macicy (p < 0.05). U samic żyjących w pobliżu elektrowni cieplnej Novaky stwierdzono największą zawartość miedzi w macicy (10.264 mg . kg[do -1]), zaś u samców w wątrobie (7.565 mg . kg[do -1]). Największa zawartość żelaza stwierdzono w macicy (618.5 mg . kg[do -1]). Zawartość żelaza w wątrobie samców była znacznie większa niż w jądrach. U samic poziom żelaza w nerkach był znacznie większy niż u samców (p < 0.01). Zawartość kadmu była największa w nerkach samic. U zwierząt z rejonu elektrowni Novaky stwierdzono większą zawartość badanych pierwiastków prawie we wszystkich badanych próbkach w porównaniu ze zwierzętami odłowionymi w rejonie elektrowni atomowej Mochovce.