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1 Medycyna Weterynaryjna

1 1990

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Zastosowanie różnych płynów inkubacyjnych do kapacytacji plemników buhaja in vitro

100%

Tizol G. , Faundez R. , Olszewski A.

Medycyna Weterynaryjna

1990

tom 46

nr 06

The experiments have been carried out with a modified Tyrode’s liquid (TALP), PBS, TALP and PBS with the addition of 1 uM of epinephrin and 10 uM of hypotaurine (TALP+, PBS+) and Ringer’s solution. There were assessed a progressive movement, morphological changes of the acrosoma and the ability of spermatozoons to penetrate the eggs of the hamster. The results showed that the solutions of TALP+ and PBS+ maintained a progressive motion of spermatozoons and increased the percentage of morphological changes in the acrosoma. Following incubation in the TALP+ there was found the highest percentage of penetrated oocytes. The findings point to epinephrin and hypotaurine' as the drugs maintaining the mobility of bull spermatozoons. No correlation was found between the percentage of changes in the acrosoma and the percentage of penetrated eggs. Such correlation was observed in case of a high percentage of spermatozoons with a progressive movement.