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This paper reports a study of the alkaloid content of different parts of Datura stramonium L. (Solanaceae) in various stages of its growth and development, and the location, structure and ultrastructure of alkaloid-biosynthesizing cells (idioblasts) in different parts of naturally grown and cultured plant material. TLC, HPLC, and GC/MS methods were used for alkaloid assays. The results showed that alkaloid production starts from the end of the second week after seed germination, increases in different organs up to the tenth week of growth, and then decreases. Leaves and capsules showed the highest alkaloid content in the vegetative and generative stages, respectively. In leaves the alkaloids decreased rapidly in the generative stage. The highest alkaloid content was recorded in vegetative leaves, followed in descending order by vegetative petioles, generative and vegetative stems, generative petioles, generative roots, generative leaves, vegetative roots and mature seeds. The organs as well as calli derived from different leaf parts were examined for the presence of idioblasts by microscopic and cytochemical methods. Idioblasts were present only in semi-hyaline callus originated from leaf base; they were spherical or oval, with a thick cell wall and large central vacuole. These observations should prove helpful in attempts to produce specific alkaloids in naturally grown plants and cell cultures.
EN
Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.
EN
This study was conducted to monitor the effectiveness of seed priming with cold plasma and supplementation of rooting medium with SiO₂ nanoparticle (nSi) on growth, physiology, and anatomy of Astragalus fridae. The plasma-primed seeds (surface power densities of 0.84 W cm⁻²; 0, 30, 60, and 90 s) were cultured in medium containing nSi (0, 5, 40, and 80 mg l⁻¹). Ultra-structural analysis manifested the nSi uptake and transportation. The high doses of bulk Si adversely affected biomass and vigour index, while response to the nSi was partially different. The nSi5 amplified the growth-promoting roles of the plasma. The nSi80 treatment slightly diminished the chlorophyll content, which was mitigated by the plasma. The carotenoid content was rectified in the plasma and/or nSi-treated seedlings. The plasma and nSi treatments led to stimulations in activities of nitrate reductase in leaves and roots. The plasma treatments provoked the drastic stimulations in the catalase activities in both roots and leaves, while the effects of nSi in these organs were contradictory. Furthermore, stem and root diameters and differentiation pattern of xylem and phloem were modified. The findings provide a theoretical basis for exploiting in plant science and related technologies. Moreover, the available plasma-generating devices could be scaled up for treating plant tissue in in vitro condition.
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