Wyniki wyszukiwania - Biblioteka Nauki

1

Grupa Bacteroides fragilis i jej znaczenie w medycynie

100%

Rokosz A.

Postępy Mikrobiologii

|

1991

|

tom 30

|

nr 1

2

Zastosowanie powierzchniowych antygenow Bacteroides thetaiotaomicron w odczynach hemaglutynacji biernej i zahamowania hemaglutynacji biernej

63%

Rokosz A. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1995

|

tom 47

|

nr 3-4

189-195

3

Zastosowanie testu immunoenzymatycznego dot-ELISA do wykrywania endotoksyny Bacteroides fragilis

63%

Rokosz A. , Meisel-Mikolajczyk F.

|

tom 34

|

nr 3

4

Wrazliwosc klinicznych szczepow bakterii Gram-dodatnich na wybrane antybiotyki beta-laktamowe

63%

Sawicka-Grzelak A. , Rokosz A.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 1-2

69-76

PL

W ciągu 4 miesięcy badań wyhodowano 111 szczepów bakterii Gram-dodat- nich od pacjentów z oddziału chirurgii ogólnej. Wśród wyizolowanych drob¬noustrojów dominowały gronkowce (49 szczepów) i enterokoki (44 szczepy). Wykryto 33 szczepy metycylinoopornych gronkowców. Penicyliny z inhibi¬torami ß-laktamaz oraz karbapenemy wykazały wysoką aktywność in vitro wobec klinicznych szczepów bakterii Gram-dodatnich z wyjątkiem E. fae- cium.

EN

The aim of the study was to determine the activity of four ß-lactam antibiotics against nosocomial strains of Gram-positive bacteria. Two antibiotics combined with ß-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in surgical ward of the National Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 STAPH, API STREP, API CORYNE and API 20 A strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB STAPH, ATB STREP and ATB ANA strips. The susceptibility of strains to timentin, tazocin, imipenem and meropenem was tested with disc diffusion method. 111 strains of Gram-positive bacteria were cultured. Staphylococci (49) and enterococci (44) dominated among isolated strains. 33 Staphylococcus spp. strains were identified as methicillin-resistant. The obtained results indicate a significant role of Gram-positive cocci (staphylococci and enterococci) in the aetiology of nosocomial infections. Antibiotics combined with ß-lactamase inhibitors and carbapenems demonstrate broad antibacterial spectrum against clinical strains of Gram-positive bacteria except E. faecium strains.

5

Wybrane cechy enterotoksynotworczych szczepow Bacteroides fragilis

63%

Rokosz A. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1995

|

tom 47

|

nr 3-4

183-188

6

Wrazliwosc klinicznych szczepow paleczek Gram-ujemnych na wybrane antybiotyki beta-laktamowe

63%

Rokosz A. , Sawicka-Grzelak A.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 3-4

191-198

EN

The aim of the study was to determine the activity of four beta-lactam antibiotics against nosocomial strains of gram-negative bacilli. Two antibiotics combined with beta-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in the following wards: surgery and intensive care unit of State Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 E and ID 32 GN strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB G- and ATB PSE strips. The susceptibility of the strains to imipenem, meropenem, timentin and tazocin was tested by disc-diffusion method. 157 strains of gram-negative bacilli were cultured. 100 strains were isolated from patients hospitalized in surgical ward and 57 strains from patients hospitalized in ICU. Nonfermenting rods dominated among isolated strains -91. The results obtained indicate that multiresistant gram-negative rods causing serious therapeutic problems are often isolated from clinical specimens. The contribution of nonfermenting rods, especially Pseudomonas spp. and Atinetobacter spp. to the etiology of infections in hospitalized patients has increased. Infections caused by these strains are difficult to cure. Tazocin and carbapenems (imipenem and meropenem) are highly active in vitro against the examined strains of gram-negative bacilli.

7

Bacteroides thetaiotaomicron strains of different origin

63%

Rokosz A. , Meisel-Mikolajczyk F.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1988

|

tom 36

|

nr 7-9

8

Wplyw nadprogowego [supra-MIC] stezenia klindamycyny na uwalnianie endotoksyny przez Bacteroides fragilis

63%

Rokosz A. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1999

|

tom 51

|

nr 1-2

143-149

9

Comparison of three types of reagents for detection of Bacteroides fragilis by direct immunoflourescence

51%

Rokosz A. , Meisel-Mikolajczyk F. , Dzielska D.

|

nr 3-4

10

Identyfikacja i lekowrazliwosc bezwzglednie beztlenowych bakterii wyizolowanych od hospitalizowanych pacjentow

51%

Kot K. , Rokosz A. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 3

233-241

PL

Od pacjentów hospitalizowanych w 2001 roku wyhodowano 225 szczepów bezwzględnych beztlenowców, w tym 54 szczepy bakterii Gram-ujemnych i 171 szczepów bakterii Gram-dodatnich. Wśród Gram-ujemnych bakterii dominowały szczepy Bacteroides fragilis, a wśród Gram-dodatnich szczepy Peptostreptococcus sp. Stwierdzono, że lekami najbardziej aktywnymi wobec klinicznych szczepów Gram-ujemnych bakterii beztlenowych były: metronidazol, piperacylina z tazobaktamem, tikarcylina z kwasem klawulanowym i imipenem, a wobec szczepów Gram-dodatnich bakterii beztlenowych: piperacylina z tazobaktamem, amoksycyiina z kwasem klawulanowym, tikarcylina z kwasem klawulanowym i imipenem.

EN

The aim of this study was to identify anaerobic strains isolated in 2001 from clinical specimens obtained from patients of Warsaw hospital and to evaluate a susceptibility of these strains to antimicrobial agents. In 2001 two hundred and twenty five clinical strains of obligate anaerobes were cultured, which were identified in the automatic ATB system (bioMérieux, France) using biochemical tests API 20 A. Drug-susceptibility of strains was determined also in ATB system with the use of ATB ANA strips. C. difficile strains were isolated on selective CCCA medium. Toxins A/B of C. difficile directly in stool specimens were detected by means of ELISA test (TechLab, USA). Fifty four strains of Gram-negative anaerobes (B. fragilis strains dominated) and 171 of Gram-positive anaerobes (the greatest number of strains belonged to genus Peptostrptococcus) were cultured from clinical specimens. In the cases of antibiotic-associated diarrhea 28 C. difficile strains were isolated and C. difficile toxins A/B were detected in 39 stool samples. The most active in vitro antimicrobials against Gram-negative anaerobes were metronidazole, imipenem, ticarcillin combined with clavulanic acid and piperacillin with tazobactam. Gram-positive, clinical strains of anaerobes were the most susceptible in vitro to ß-lactam antibiotics combined with ß-lactamase inhibitors (amoxicillin/clavulanate, piperacillin/tazobactam, ticarcillin/clavulanate) and imipenem.

11

Zastosowanie czterech wariantow testu krazka diagnostycznego [CD01, CD02, CD03, CD04] do wykrywania ESBL-dodatnich szczepow Gram-ujemnych paleczek

51%

Rokosz A. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2005

|

tom 57

|

nr 1

77-83

PL

Przedmiot badań stanowiło 111 klinicznych szczepów Gram-ujemnych pałeczek wytwarzających beta-laktamazy o rozszerzonym spektrum substrato- wym (ESBLs), dodatnich w teście dwóch krążków (DDST-dodatnie). Wszystkie szczepy zostały wyhodowane w 2004 roku. W celu porównania wyników oznaczeń zastosowano cztery warianty testu krążka diagnostycznego (DD) z użyciem krążków CD01, CD02, CD03 i CD04 firmy Oxoid Ltd. Zbieżność wyników z metodą DDST byla następująca: CPD/CD01 - 59 szczepów (53,2%), CAZ/CD02 - 80 szczepów (72,1%), CTX/CD03 - 92 szczepy (82,9%) i CPO/CD04 - 110 szczepów (99,1%). Przy użyciu krążka CD04 otrzymano najwyższą zgodność rozpoznawania ESBLs w porównaniu z metodą DDST.

EN

The aim of this study was to evaluate the usefulness of four variants of the diagnostic disc test (DD) to detect extended-spectrum beta-lactamases (ESBLs) in nosocomial strains of Gram-negative rods. Also, the diagnostic disc test (DD) was compared with the double-disc synergy test (DDST) for the effectivity of ESBLs identification A total number of 111 ESBL-positive (DDST-positive) strains of Gram-negative rods isolated from hospitalized patients in 2004 was examined. Ninety nine strains belonged to enteric rods (89.2%) and twelve strains - to nonfermentative rods (10.8%). Two reference strains: E. coli ATCC 25922 (ESBL-negative one) and K. pneumoniae ATCC 700603 (ESBL-positive one) were included in the study. Four variants of the diagnostic disc test (DD, Oxoid Ltd, UK) were applied for ESBLs detection: CPD/CD01, CAZ/CD02, CTX/CD03 and CPO/CD04. All examined strains (111) were DDST-positive. Positive results in the DD test (Oxoid Ltd) were as follows: CPD/CD01 - 59 strains (53.2%), CAZ/CD02 - 80 strains (72.1%), CTX/CD03 - 92 strains (82.9%) and CPO/CD04 - 110 strains (99.1%). Discs containing cefpirome (CPO) and cefpirome with clavulanic acid (CD04) were the best set for detection of ESBLs in our collection of clinical Gram-negative rods. Results of this variant of the DD test were the most consistent with the results of the DDST. Application of several disc diffusion methods to detect ESBL producers increases the probability of proper identification of these strains.

12

ESBL-dodatnie szczepy z grupy Bacteroides fragilis wyizolowane od pacjentow Wojewodzkiego Szpitala Zespolonego w Plocku

51%

Rokosz A. , Pawlowska J. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2004

|

tom 56

|

nr 3

245-253

13

Wykrywanie endotoksyny i enterotoksyny Bacteroides fragilis w podlozu hodowlanym

51%

Rokosz A. , Kruszewska D. , Rouyan G. S. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 1-2

61-67

EN

Four B. fragilis strains were studied nonenterotoxigenic (NTBF) and three enterotoxigenic (ETBF). Endotoxin and enterotoxin which are released into the culture medium during the growth of strains were detected. Cultures in BHI broth were incubated for 48 hours at 37ºC. After 4, 8, 16, 24 and 48 hours of cultivation, samples of bacterial culture were colleted and the optical density was measured. Then the samples were centrifuged, supernatans were filtered through 0.45 um filters and concentrated three times with 5000 D ultrafilters. Prepared samples were kept frozen at 71ºC until use. The presence of endotoxin in samples was revealed by means of immunoelectroprecipitation (IEP) and immunoenzymatic test (dot-ELISA). The assays were performed with antibacterial rabbit immune sera. The activity of enterotoxin was detected on a human colon adenocarcinoma cell line HT 29/C1. The results of the study indicate that endotoxin is released spontaneously by nonenterotoxigenic IPL E 323 strain into the culture medium at the early stages of cultivation. The presence of endotoxin is not demonstrated by means of immunoelectroprecipitation in culture filtrated of ETBF strains. Trace amounts or endotoxin are revealed with dot-ELISA. The activity of enterotoxin is detected after 16 hours of incubation of ETBF strains.

14

Szczepy z rodzaju Salmonella wyizolowane z przypadkow zakazen pozajelitowych

51%

Lazinska B. , Rokosz A. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2005

|

tom 57

|

nr 3

287-294

PL

W latach 2002-2004 z próbek materiału klinicznego wyizolowano 13 szczepów z rodzaju Salmonella, w tym 11 szczepów S. Enteritidis i 2 szczepy S. Hadar. Wszystkie wyhodowania dotyczyły zakażeń pozajelitowych. Wyhodowane szczepy z jednym wyjątkiem były w pełni wrażliwe na antybiotyki i chemioterapeutyki. Nie wyhodowano szczepów ESBL – dodatnich.

EN

Every year in Poland from tens to more than hundred bacteriologically verified extraintestinal infections caused by Salmonella species have been registered. These unusually located infections have substantially heavy course and in many cases hospitalisation and antibiotic therapy have to be involved. Cases of extraintestinal infections with these Gram-negative rods, which were described in the literature, concerned: pneumonias, lung abscesses and thoracic empyemas, and infections of: blood, bones and joints, wounds, fistulas and urinary tract. The aim of this study was to set extraintestinal Salmonella infections and to analyze a susceptibility of isolated strains to antimicrobial agents. Between 01.07.2002 and 31.12.2004 (30 months) 13 strains of Salmonella genus have been isolated, including 11 S. enteritidis and 2 S. Hadar. In general, with one exception, isolated strains were susceptible to tested antibiotics/chemotherapeutics. ESBL - positive strains were not detected. The tendency of Salmonella strains to cause extraintestinal infections has been noticed. The problem is still escalating, especially in group of patients chronically treated, with immunodeficiency and immunosuppression, after complicated medical procedures, also in the group of small children and aged persons. Therefore, it is necessary to evaluate a susceptibility to antibiotics/chemotherapeutics of every strain from confirmed case of Salmonella extraintestinal infection and it is important to apply a guided therapy.

15

Lekowrazliwosc 100 klinicznych szczepow Enterococcus spp.

51%

Sawicka-Grzelak A. , Rokosz A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

1999

|

tom 51

|

nr 3-4

239-247

16

Zastosowanie nowej metody do wykrywania beta-laktamaz o rozszerzonym spektrum substratowym [ESBLs]

51%

Rokosz A. , Sawicka-Grzelak A. , Meszaros J. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 3

225-231

PL

Z próbek materiału klinicznego wyosobniono 260 szczepów Gram-ujemnych pałeczek, które zidentyfikowano jako ESBL-dodatnie metodą dwóch krążków (DDST). Wszystkie szczepy oznaczono za pomocą nowej metody (DD) służącej do wykrywania beta-laktamaz o rozszerzonym spektrum substratowym z użyciem krążka z cefpodoksymem oraz krążka diagnostycznego z cefpodoksymem i kwasem klawulanowym (CD 01). Zgodność wyników oznaczeń dla obu metod dotyczyła 60,4% badanych szczepów.

EN

The aim of performed examinations was to compare results of two methods applied for the detection of extended-spectrum beta-lactamases (ESBLs). Two hundred and sixty strains of Gram-negative rods were cultured from clinical specimens obtained from hospitalized patients. These strains were identified as ESBL-positive on the basis of double-disc method (DDST) according to Jarlier et al. (1988) results. Identification of strains performed in automatic ATB Expression system (bioMerieux, France). All strains were determined using a novel test for detection of ESBL-type enzymes (DD) according to Appleton (1999). Two discs are applied in this test: with cefpodoxime (CPD) and with cefpodoxime/clavulanate (CD 01, diagnostic disc). Consistent results of two methods (DDST and DD) were obtained in the case of 166 from among 260 of examined strains (60.4%). Consistent results concerned 161 out of 222 examined strains of enteric rods (72.5%) and only 5 from among 38 of other strains (mostly belonging to the group of non-fermenting rods). On the basis of performed investigations it can he stated that the novel method of extended-spectrum beta-lactamases (ESBLs) detection (DD) according to Appleton (1999) is more objective and easier for interpretation than the double-disc synergy test (DDST) according to Jarlier et al. (DDST), which is widely applied in the routine microbiological diagnostics.

17

Ultrastructure of Bacteroides thetaiotaomicron cells

51%

Rokosz A. , Hassa Z. , Meisel-Mikolajczyk F. , Wasiutynski A.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1989

|

tom 37

|

nr 7-9

18

The toxicity of Bacteroides thetaiotaomicron cell-surface antigens to chicken embryos

51%

Rokosz A. , Kruszewska D. , Rumin W. , Meisel-Mikolajczyk F.

|

1990

|

tom 39

|

nr 3-4

19

Porownanie wynikow krazkowo-dyfuzyjnych metod stosowanych do wykrywania ESBL-dodatnich szczepow paleczek Gram-ujemnych

51%

Rokosz A. , Sawicka-Grzelak A. , Meszaros J. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2004

|

tom 56

|

nr 1

49-55

PL

Przedmiot badań stanowiło 120 klinicznych szczepów ESBL-dodatnich pałeczek Gram-ujemnych. Aktywność ESBL u tych szczepów wykryto przy zastosowaniu dwóch metod: DDST (metoda dwóch krążków) i DD (metoda krążka diagnostycznego). W teście DD użyto krążków z cefpodoksymem oraz z cefpodoksymem i kwasem klawulanowym (pierwszy wariant - CPD/CD 01). Następnie szczepy sprawdzono pod kątem wytwarzania ESBL stosiyąc kolejne dwa warianty metody krążka diagnostycznego: drugi (CAZ/CD 02) i trzeci (CTX/CD 03). W przypadku kilku szczepów zaobserwowano niezgodności wyników oznaczeń wykonanych za pomocą różnych metod krążkowo-dyfuzyjnych.

EN

Examinations were undertaken to compare the results of disc diffusion tests applied for detection of strains producing extended-spectrum beta-lactamases (ESBLs). A total of 120 clinical strains were used in experiments. These strains were determined as ESBL-positive on the basis of consistent results of two methods: the double disc synergy test (DDST) according to Jarlier et al. (1988) and the diagnostic disc test (DD, version CPD/CD 01) according to Appleton (1999). In the next step examined strains were analysed in two further tests, which are variants of DD method: CAZ/CD 02 test with discs containing ceftazidime and ceftazidime/clavulanic acid, and CTX/CD 03 test with the use of cefotaxime and cefotaxime/clavulanic acid discs. ESBL-positive strains first of all belonged to the species E. coli and K. pneumoniae. In the case of seven analysed strains consistent results of determinations were not obtained with the use of different disc diffusion methods. Application of several disc diffusion methods to determine ESBL-positive strains of Gram-negative rods increases the probability of their proper identification.

20

Paleczki Gram-ujemne wytwarzajace metalo-beta-laktamazy izolowane od pacjentow szpitalnych i pozaszpitalnych, wykryte przy zastosowaniu metody Etest MBL

51%

Rokosz A. , Sawicka-Grzelak A. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2006

|

tom 58

|

nr 1

73-81

PL

W latach 2001-2005 z próbek materiału klinicznego pobranych od pacjentów szpitalnych i ambulatoryjnych wyizolowano 104 szczepy Gram-ujemnych pałeczek opornych na karbapenemy (imipenem i meropenem). Zastosowano fenotypową metodę Etest® MBL do wykrywania szczepów wytwarzających metalo-beta-laktamazy (MBL). Wśród badanych izolatów było 36 MBL-do- datnich stanowiących 34,6% ogółu szczepów opornych na antybiotyki kar- bapenemowe.

EN

The aim of presented study was to detect MBL-positive strains in a group of clinical carbapenem- resistant strains isolated from inpatients and outpatients during last four years. From the beginning of November 2001 to the end of October 2005, one hundred and four strains resistant to carbapenem antibiotics - imipenem and meropenem were cultured from clinical samples obtained from patients of the Infant Jesus Clinical Hospital Centre for Trauma Treatment in Warsaw and from patients of outpatient clinics. Strains were identified and their susceptibility to antibacterial agents was determined in the automatic ATB Expression system (bioMérieux®). Resistance to imipenem and meropenem was confirmed with a disc diffusion method. Production of metallo-beta-lactama- ses (MBL) was examined with the use of Etest® MBL (AB Biodisk, Sweden), and extended-spectrum beta-lactamases (ESBL) by means of following procedures: DDST and / or DD (four variants) (Oxoid Ltd., England). MBL-positive strains (36) were cultured in cases of infections in adult patients (35 strains) and in a child (1 strain). Majority of strains belonged to the species P. aeruginosa (27), several strains - to the species P. putida (6) and remaining strains - to P. stutzeri, A. xylosoxidans, and E. cloacae ( 1 strain of each species). Four strains were producers of MBL-type and ESBL-type beta-lactamases. According to our knowledge and accessible literature described strains (except one paediatric strain) are the first MBL-positive strains isolated from adult hospitalized patients and adult ambulatory patients in Poland. Additionally, MBL-positive E. cloacae strain is probably the first MBL producer isolated in Poland, which belongs to the group of enteric rods. MBL-producing strains of Gram-negative rods, detected by phenotypic Etest® MBL method, will be verified with genetic procedures.