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3 Journal of Applied Genetics

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Restriction fragment length polymorphism of exon 2 Ovar-DRB1 gene in Polish Heath Sheep and Polish Lowland Sheep

100%

Gruszczynska J. , Brokowska K. , Charon K. M. , Swiderek W. P.

Journal of Applied Genetics

2005

tom 46

nr 3

311-314

Exon 2 of the Ovar-DR gene is known to encode the MHC outer domain (alpha or beta chain) that forms the binding area to antigens presented. The study was aimed at analysing exon 2 Ovar -DRB1 gene polymorphism in Polish Heath Sheep and Polish Lowland Sheep (Zelazna variety). A total of 101 and 99 ewes of the respective breeds were included in this study. We identified 65 different haplotypes in Polish Heath Sheep and 68 in Polish Lowland Sheep. The PCR-RFLP method and PCR products sequencing made it possible to identify two new sequences of exon 2 Ovar-DRB1 gene (AY230000 and AY248695). A distinct polymorphism in the exon 2 sequence presents possibilities for immune response toward a great variety of pathogens.

The influence of OLA-DRB1 (MHC class II) gene polymorphism on lamb body weight and weight gain in Polish Heath Sheep

100%

Gruszczynska J. , Charon K. M. , Kitlinska J. , Szydlowski M.

Journal of Applied Genetics

2000

tom 41

nr 2

101-112

The study aimed at analysing the OLA-DRB1 (exon2/microsatellite sequence in intron2) gene polymorphism in Polish Heath Sheep and the influence of particular alleles on lamb body weight and weight gain. A total of 45 ewes, 7 rams and 100 lambs of Polish Heath Sheep were examined. In the parental generation 36 alleles of the OLA-DRB1 were found and the most frequent were 466bp, 500bp and 566bp (0.058, 0.125 and 0.067, respectively). In the investigated lambs there were 30 alleles with the highest frequency of 498bp, 500bp, 560bp and 566bp alleles (0.105, 0.210, 0.075 and 0.085, respectively).The presence of certain OLA-DRB1 alleles in ewe and lamb genotypes was found to be associated with significant differences in lamb rearing results. The significant or highly significant influence on body weight at birth and weight gain during the first month of lamb's life were observed in the case of the following alleles: 464bp, 466bp, 470bp, 488bp, 494bp, 538bp and 576bp in lambs; 468bp, 470bp, 474bp, 514bp, 536bp and 548bp in ewes.

Microsatellite polymorphism in locus OMHC1 (MHC Class I) in Polish Heath Sheep and Polish Lowland Sheep (Zelazna variety)

100%

Gruszczynska J. , Charon K. M. , Swiderek W. , Sawera M.

Journal of Applied Genetics

2002

tom 43

nr 2

217-222

Our study aimed at comparative analysis of microsatellite polymorphism in locus OMHC1 (MHC Class I) in Polish Heath Sheep and Polish Lowland Sheep (Zelazna variety). The study was conducted on 100 ewes of each breed. We identified 13 alleles of the gene in Polish Heath Sheep and 9 in Polish Lowland Sheep. We found marked differences in frequency of OMHC1 alleles between both breeds. The heterozygosity coefficient and PIC, amounting to 0.79 and 0.77 for Polish Heath Sheep, and 0.82 and 0.80 for Polish Lowland Sheep, respectively, suggest considerable variability in both breeds. Additionally, the values of both coefficients indicate that OMHC1 locus can be used as a genetic marker.