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4 Acta Physiologiae Plantarum

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Influence of selected carbohydrates on rhizogenesis of shoots saucer magnolia in vitro

100%

Kamenicka A.

nr 4

Nodal segements were taken from juvenile shoots of mature 100 year-old trees of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) and cultured on Standardi and Catalano medium supplemented with 1.33 µmol·dm⁻³ BA, 0.54 µmol·dm⁻³ NAA, 58 µmol·dm⁻³ sucrose and 6.0 g·l⁻¹ agar-agar. After 8 weeks, separated shoots were transferred to rooting medium with half-strength macronutrients (basal medium) supplemented with 0.3% activated charcoal and one of carbohydrates: arabinose, cellulose, fructose, galactose, glucose, lactose, mannose, rhamnose, ribose, sorbose, sucrose or xylose at 20 g·dm⁻³ and 7.0 g·dm⁻³ agar-agar. After 13 weeks of culture, shoot number, fresh and dry weight of shoots and roots, total root length and number of roots/per shoot were recorded. Percentages of rooted shoots were calculated. Fructose, mannose and xylose were the most effective carbon source on shoot proliferation followed by sucrose. The rooting response was induced by cellulose and xylose. Arabinose, rhamnose and sorbose inhibited root formation. The number of adventitious roots produced per shoot was stimulated by cellulose and xylose. Total biomass (shoot plus roots) of the plantlets was the highest at fructose and cellulose.

Influence of culture conditions on growth parameters and pigments content during acclimatisation of plantlets

63%

Kamenicka A. , Kubova A.

nr 3 suppl.

Effect of medium composition and type of vessel closure on axillary shoot production of magnolia in vitro

63%

Kamenicka A. , Lanakova M.

Acta Physiologiae Plantarum

2000

tom 22

nr 2

A comparative study of different cytokinins on the formation of Rhododendron forrestii Balf.f.ex Diels. axillary shoots in vitro

51%

Kamenicka A. , Valka J. , Vizarova G.

tom 20

nr 2

The effect of different concentrations and activities of cytokinins on the morphogenesis of regenerated Rhododendron forrestii Balf. f. ex Diels. shoots taken from nodal segments were tested. We evaluated zeatin, zeatin riboside, izopentyladenine, izopentyladenine riboside, kinetin, kinetin riboside, benzylaminopurine, benzylaminopurine riboside. The experimental results were evaluated by mathematical methods and regression analysis describing the effect of isoprenic and aromatic type of cytokinins. On the basis of this modelling, maximum axillary shoot production was attained with medium supplemented with 2.0 mg·l⁻¹ izopentyladenine riboside, 2.0 mg·l⁻¹ benzylaminopurine and 20 g·l⁻¹ sucrose. Minimal axillary shoots were produced with kinetin and kinetin riboside.