The paper reviews the aspects of physiology and biochemistry of microbial biodegradation of the main components of petroleum oil: alkanes (larger than methane), alkenes, alkines, cyclic, polycyclic, aromatic and polyaromatic hydrocarbons. It focuses on aerobic degradation pathways. The review provides key information prepared on the basis of more than 30 years of research on microbial degradation of hydrocarbons. The areas discussed include new pathways of biodegradation of branched-chain alkanes, cyclic and polyaromatic hydrocarbons which have been discovered during the past few years. Special attention was paid to oxygenases ? enzymes initiating aerobic metabolism of hydrocarbons and phenomenon of co-oxidation which enables assimilation of most recalcitrant components of crude oil.
The present state of science in microbial (classical) biotechnology in Poland is described.The main areas of research discussed in this paper include selection and industrial microorganisms improvement, optimization of biosynthesis processes, engineering aspects, techniques of immobilising cells and enzymes, processes of biotransformation and biodegradation, downstream processing and biotechnology in environmental protection.Special attention is paid to the problems of collaboration of scientific institutes with industry.The most important results in the above mentioned fields are also presented.
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