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Urinary tract infections of Escherichia coli strains of chaperone-usher system

100%

Zalewska-Piatek B. M.

Polish Journal of Microbiology

2011

tom 60

nr 4

Urinary tract infections are a very serious health and economic problem affecting millions of people each year worldwide. The most common etiologic agent of this type of bacterial infections, involving the upper and lower urinary tract, are E. coli strains representing approximately 80% of cases. Uropathogenic E. coli strains produce several urovirulence factors which can be divided into two main types, surface virulence factors and exported virulence factors. Surface-exposed structures include mainly extracellular adhesive organelles such as fimbriae/pili necessary in adhesion, invasion, biofilm formation and cytokine induction. Among the surface-exposed polymeric adhesive structures there are three most invasive groups, type 1 pili, type P pili and Dr family of adhesins which are bioassembled via the conserved, among Gram-negative bacteria, chaperone-usher secretion system. Type 1 and P-piliated E. coli cause cystitis and pyelonephritis. The Dr family of adhesins recognizing DAF receptor is responsible for cystitis, pyelonephritis (especially in pregnant women) and diarrhoea (in infants). In addition, Dr-positive E. coli strains carry the risk of recurrent urinary tract infections. Pyelonephritis in pregnant women leads to a series of complications such as bacteremia, urosepsis, acute respiratory distress syndrome and even death. In the era of increasing drug resistance of bacteria, the development of vaccines, drugs termed pilicides and inhibitors of adhesion may be a promising tool in the fight against urogenital infections.

Alternative treatment approaches of urinary tract infections caused by uropathogenic Escherichia coli strains

63%

Zalewska-Piatek B. M. , Piatek R. J.

Acta Biochimica Polonica

2019

tom 66

nr 2

Biofilm formation as a virulence determinant of uropathogenic Escherichia coli Drplus strains

51%

Zalewska-Piatek B. M. , Wilkanowicz S. I. , Piatek R. J. , Kur J. W.

nr 3

223-229

Urinary tract infections are the most common health problem affecting millions of people each year. Uropathogenic Escherichia coli (UPEC) strains are the major factor causing lower and upper urinary tract infections. UPEC produce several virulence factors among which are surface exposed adhesive organelles (pili/fimbriae) responsible for colonization, invasion and amplification within uroepithelial cells. The virulence of the uropathogenic E. coli Dr⁺ IH11128 is associated with Dr fimbriae belonging to the Dr family of adhesins (associated with diarrhea and urinary tract infections) and a DraD protein capping the linear fiber at the bacterial cell surface. In this study we revealed that biofilm development can be another urovirulence determinant allowing pathogenic E. coli Dr⁺ to survive within the urinary tract. E. coli strains were grown in rich or minimal media, allowed to adhere to abiotic surfaces and analyzed microscopically by staining of cells with cristal violet. We found that both Dr fimbriae and DraD, exposed at the cell surface in two forms, fimbria-assoeiated or fimbria non-associated, (DraE⁺/DraD⁺, DraE⁺/DraD⁻ or DraE⁻/DraD⁺ E. coli strains) are required for biofilm formation. Additionally, we demonstrated the biofilm formation capacity of E. coli strains deficient in the surface secretion or production of the DraE adhesin.