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1 Acta Chromatographica

1 2007

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Quantitative analysis of valsartan and hydrochlorothiazide in tablets by high performance thin-layer chromatography with ultraviolet absorption densitometry

100%

Kadam B. R. , Bari S. B.

2007

tom No. 18

260-269

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of valsartan and hydrochlorothiazide in tablet formulations. Standard and sample solutions of valsartan and hydrochlorothiazide were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with chloroform–ethyl acetate–acetic acid, 5:5:0.2 (v/v), as mobile phase. UV detection was performed densitometrically at 248 nm. The retention factors of valsartan and hydrochlorothiazide were 0.27 and 0.56, respectively. The linear range was 800–5600 ng per spot for valsartan and 125–875 ng per spot for hydrochlorothiazide; the correlation coefficients, r, were 0.9998 and 0.9988, respectively. The method was validated in accordance with the requirements of ICH guidelines and was shown to be suitable for purpose. The method was successfully used for determination of the drugs in tablets. Tablet excipients did not interfere with the chromatography.