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Flow karyotyping and chromosome sorting in plants

100%

Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

Application of flow cytometry for the study of plant genomes

100%

Dolezel J.

Journal of Applied Genetics

1997

tom 38

nr 3

During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement of our knowledge of plant genome structure and function. This paper reviews the applications of flow cytometry for the analysis of isolated nuclei and chromosomes. Because of its speed, precision and convenience, this method of analysis of the nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained with chromosome analysis and sorting indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.

Karyological characterization of sugar beet gynogenetic lines cultured in vitro

63%

Svirshchevskaya A. , Dolezel J.

nr 1

Molecular cytogenetics of Musa spp.

51%

Dolezelova M. , Valarik M. , Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

Cytogenetic variation in tissue culture-derived Hypericum perforatum L. plants and their seed progenies

51%

Brutovska R. , Cellarova E. , Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

Flow cytometric analysis of nuclear genome size in Musa spp.

51%

Lysak M. , Dolezelova M. , Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

Localisation of GAA microsatellites on barley chromosomes by direct cycling-PRINS [C-PRINS]

51%

Kubalakova M. , Cihalikova J. , Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

Cell cycle synchronisation and chromosome isolation in barley [Hordeum vulgare]

51%

Cihalikova J. , Lysak M. , Dolezel J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

1998

tom 1696

The bacterial artificial chromosome [BAC] library of the narrow-leaved lupin [Lupinus angustifolius L.]

38%

Kasprzak A. , Safar J. , Janda J. , Dolezel J. , Wolko B. , Naganowska B.

nr 3

The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144×384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant’s genome structure and evolution.