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Trzesawka - pasazowalna encefalopatia gabczasta owiec

100%

Kaczor U. , Domon D.

Wiadomości Zootechniczne

2009

tom 47

nr 3

37-45

Polymorphism in the PRNP locus in prolific Olkuska sheep

80%

Kaczor U. , Domon D. , Martyniuk E. , Murawski M.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 1

PCR-RFLP method was used to identify polymorphisms in the 136 (A/V), 154 (R/H), and 171 (R/H/Q) codons of the prion protein gene (PRNP) in 174 ewes and rams of the prolific Olkuska sheep breed from three nucleus flocks. Alanine (A) at codon 136, arginine (R) at codon 154, and arginine (R) or glutaminę (Q) at codon 171, responsible for the presence of two alleles (ARR and ARQ) and three genotypes, (ARR/ARR, ARR/ARQ and ARQ/ARQ) were found in the analysed population. In all flocks, ARR allele (the most resistant to scrapie) was the dominant haplotype regardless of sheep sex, and ARR/ARQ genotype had the highest frequency (60.92%). The proportion of the undesirable ARQ/ARQ genotype was only 4.02%. Simulation of genotype distribution for the next generations showed that the mating of ewes with ARR/ARR genotype rams will cause this genotype to appear in 99% of Olkuska ewes already in the sixth generation. However, the study showed no relationship between genotype in the PRPN locus and prolificacy potential of the ewes.