Wyniki wyszukiwania - Biblioteka Nauki

1

Kriogeniczne przechowywanie nasion wybranych gatunkow roslin uzytkowych

100%

Niedzielski M. , Puchalski J.

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tom 463

255-267

PL

O możliwościach efektywnego zamrażania nasion roślin uprawnych w ciekłym azocie decydują parametry zamrażania takie jak: szybkość mrożenia i wilgotność nasion, a także budowa i skład chemiczny nasion. Spośród badanych gatunków nasion roślin uprawnych: motylkowych, zbóż, warzyw z rodziny baldaszkowatych w większości przypadków możliwe było zamrażanie w szerokim zakresie szybkości mrożenia i wilgotności nasion. Jednakże w odniesieniu do soi konieczne było określenie specyficznych warunków mrożenia, a dla żyta wyniki pokazały istnienie krytycznej zawartości wody w nasionach warunkującej efektywne zamrażanie tych nasion.

EN

Effectiveness of crop germplasm storage in liquid nitrogen predominantly depends on the freezing parameters like the rate of freezing and seed moisture content. Also seed morphology and chemical composition play an important role. Among the investigated crop seed species of cereals, leguminous and vegetables, in most cases it was possible to freeze the seed samples within a wide range of freezing rate and seed moisture content. However, it was necessary to find the specific frezing parameters for soybean seed. Moreover, rye seed freezing experiment revealed an existence of a threshold water content in seeds affecting their effective cooling.

2

Starzenie się nasion - podstępny wróg zachowania różnorodności biologicznej w bankach genów

80%

Boczkowska M. , Rucinska A. , Targonska-Karasek M. , Niedzielski M.

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nr 283

3

The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation

80%

Mikula A. , Niedzielski M. , Rybczynski J. J.

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nr 4

EN

This study was aimed at improving the 2,3,5-triphenyltetrazoliumchloride (TTC) reduction test for initial assessment of cell survival after cryopreservation. Experiments were carried out on three embryogenic cell suspensions of different ages: 9-year-old Gentiana tibetica (King ex Hook. F.), 2-year-old G. kurroo (Royle), and 1-year-old G. cruciata (L.). The suspensions were maintained in MS medium supplef mented with 1.0 mgl-1 3,6-dichloro-o-anisic acid, 0.1 mgl-1 naphthaleneacetic acid, 2.0 mgl-1 6-benzylaminopurine, 80.0 mg l-1 adenine sulphate and 0.09 M sucrose. Four weeks before freezing, part of the tissue was subcultured to the same medium with sucrose concentrations elevated from 0.09 M (3%sMS) to 0.175 M (6%sMS) or 0.26 M (9%sMS). In freezing treatments without cryoprotection, tissue was plunged directly into liquid nitrogen (LN) or cooled gradually. In freezing treatments with cryoprotection, the cells were pretreated with 1 M sucrose, or with 0.4 M sorbitol + 0.25 M pro line or + 0.08 M DMSO, or with vitrification solufion (PVS2). Encapsulation was another variant. TTC reduction activity was spectrophotometrically assessed immediately, 1,3,5,24 and 48 h after thawing. Cells without cryoprotection were lethally damaged, but TTC reduction activity in those cells ranged from 6.5 % (tissue from 3%sMS) to 73 % (tissue from 9%sMS) directly after thawing. Formazan production was reduced to zero afier 24 h. The TTC test showed 50 % formazan content immediately after thawing of DMSO-protected G. tibetica tissue, but only 22.47 % after 24 h and 2.9 % afier 48 h. Ultrastructural analysis of those cells showed lethal damage in many of them. For the PVS2 treatment, the formazan confent was similar in samples analyzed directly after thawing and 24 h later. Cells treated with PVS2 did not show structural disturbances. Encapsulated cell aggregates of G. cruciata treated with concentrations of sucrose increasing up to 1 M produced 2.6 times more formazan. When applied at least 48 h after thawing, the TTC test can reflect cell viability and can be used to compare the effectiveness of cryoprotectant performance and freezing protocols, but it must be carefully evaluated, with appropriate controls.

4

Comparative studies on Polish and Italian populations of Dactylis glomerata subsp. glomerata [Poaceae]

80%

Mizianty M. , Cenci C. A. , Niedzielski M. , Bednarek P.

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nr 2

5

TTC test functionality in evaluation of surviving of Gentiana pem after cryopreervation

71%

Mikula A. , Niedzielski M. , Fiuk A. , Zielinska M. , Rybczynski J. J.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3 suppl.

6

Starzenie się nasion - złożony problem banków genów. Praca przeglądowa

61%

Boczkowska M. , Rucinska A. , Targonska-Karasek M. , Olszak M. , Niedzielski M. , Rakoczy-Trojanowska M.

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nr 4