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1

Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.)

100%

Borzym E. , Maj-Paluch J.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

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nr 4

EN

The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.

2

Diagnostyka molekularna herpeswirusa koi (KHV)

80%

Maj J. , Borzym E. , Matras M.

Życie Weterynaryjne

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2011

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tom 86

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nr 12

3

Wpływ utrwalaczy organicznych na stabilność materiału genetycznego DNA herpeswirusa koi (KHV)

80%

Borzym E. , Maj J. , Matras M.

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nr 06

EN

Studies to identify the koi herpes virus using the PCR are continuously developed and introduced into routine diagnostics. Well-selected ways of storing tissues that are later used for DNA extraction and for the amplification reaction are critical points in methods using molecular biology techniques. The objective of the studies was to determine the impact of the fixating properties of the two most frequently used organic compounds, ethanol 96% and 80%, and isopropanol, on the stability of the nucleic acid isolated and on the preservation of the koi herpes virus in selected tissues and organs of carps. The experimental fish were infected via a bath in a small 20 l aquarium, where 5 ml of a KHV reference strain suspension had been added to the water. The presence and stability of the total DNA isolated from tissues and organs (skin, brain, kidney, gills) was checked using PCR with TK primers designed by Bercovier, and a product of 409 bp was obtained. Primers amplifying a gene fragment of beta-actin, which is found in smooth muscles and non-muscle cells of fish, were used as isolation control in multiplex PCR. On the basis of the studies it was observed that just on the day the samples were taken from the internal organs of fish and a month later, when they were collected from the archived organs, a product of 409 bp and 259 bp was obtained in all the samples analysed. The best organic fixative of organs and tissues sampled for molecular tests to identify KHV is ethanol 96%, even after half a year of archiving. Ethanol 80% and isopropanol also ensure sufficient fixation for up to a month. The most stable virus DNA is isolated from fish skin and gills.

4

Choroby ryb jesiotrowatych

80%

Borzym E. , Wlasow T. , Fopp-Bayat D.

Życie Weterynaryjne

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2016

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tom 91

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nr 06

5

Epizootyczny zespol wrzodowy - egzotyczna choroba ryb objeta obowiazkiem zglaszania

80%

Antychowicz J. , Borzym E. , Matras M.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 07

450-454

EN

Epizootic ulcerative syndrome (EUS) is a dangerous fish infection with the water fungus Aphanomyces invadans. It occurs in Asia, Australia and America, in the Florida region, and affects many inland fish species. In accordance with Council Directive 2006/88/EC, EUS is one of the exotic notifiable diseases in Europe. In the paper an attempt was made to answer the question concerning the possibility of Aphanomyces invadans infection occurring in fish reared in Poland. In order to answer this question, the analysis of the fungus biology was performed, the factors predisposing to the infection were evaluated and the EUS-susceptible fish species were presented. If the theory of climate warming is true, a gradual extension of the area inhabited by the stenothermal Aphanomyces invadans seems possible. Even now thermal water conditions in ponds fed with electric plant cooling water in Europe approximate those in Asiatic natural water basins. Many popular Asiatic aquarium fish species are susceptible to Aphanomyces invadans or could be carriers of this fungus. These fishes are a potential source of infection at least to some European fishes. Considering even the slightest possibility of Aphanomyces invadans introduction in Poland followed by EUS occurrence, the authors proposed some necessary preventive measures. Among others, the importation of aquarium fish species which are susceptible to EUS should be prohibited. Also certain legislative regulations of carp (Cyprinus carpio) movement for stocking purposes should be applied.

6

Potential role of different fish species as vectors of koi herpesvirus (CyHV-3) infection

71%

Matras M. , Stachnik M. , Borzym E. , Maj-Paluch J. , Reichert M.

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2019

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tom 63

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nr 4

7

Disturbances of cow oocyte maturation by phytoestrogens

71%

Borzym E. , Bobowiec R. , Kosior-Korzecka U. , Martelli F. , Burmanczuk A.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 09

EN

The aim of the study was to elucidate the influence of different phytoestrogens (PEs) such as izoflavons (represented by genistein), stilbens (represented by resveratrol (RES)), lignans (represented by enterodiol) and coumestans (represented by coumstrol) on the maturation process of isolated bovine oocytes. We assumed that their influence on the rate of oocyte growth and maturation would be related primarily to their estrogenic activity (isoflavones and coumestans are similar to the 17β estradiol - E₂, the most potent oestrogen). Eighty to one hundred oocytes were cultured in 4 ml of the medium in six-well multidishes. Cumulus oocyte complexes (COCs) were matured at 39°C in humidified air containing 5% CO₂ for 24 hours. Four phytoestrogens were tested at two concentrations: 10 µg/ml and 100 µg/ml. These results clearly show that there is no significant relation between PEs and the survival of cow oocytes. However, under the influence of different PEs the expansion of oocytes was suppressed, especially when genistein and coumestrol were introduced into the culture medium. It was noted that the addition of the phytoestrogens into the medium decreased the rate of oocyte maturation. The highest increase of immature oocytes (p ≤ 0.05) was noted after the addition of coumestrol (30.77%) and genistein (20%) (fig. 4). The most pronounced suppression of oocyte maturation by genistein and coumestrol observed in our research indicates, therefore, that there is a direct relationship between the potency of the estrogenic activity of particular PEs and the inhibition of oocyte maturation. We concluded from our studies that PEs may disturb the conception and fertilisation rate in the cow by prolonging cow oocyte maturation.

8

Najgroźniejsze wirusy ryb hodowlanych w obiektach RAS i możliwość ich zwalczania

71%

Matras M. , Stachnik M. , Borzym E. , Maj-Paluch J. , Reichert M.

Magazyn Przemysłu Rybnego

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2018

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nr 2

9

First laboratory confirmation of salmonid alphavirus type 2 (SAV2) infection in Poland

71%

Borzym E. , Maj-Paluch J. , Stachnik M. , Matras M. , Reichert M.

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tom 58

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nr 3

EN

The aim of the study was to identify the genotype of Polish isolates of salmonid alphaviruses (SAV) and to find the origin of the virus. Samples for virus isolation included the kidneys, spleen, and liver pooled from 10 fish. A typical cytopathic effect was observed after inoculation of samples on cell lines. Total RNA was extracted from cell culture supernatant and submitted to RT-PCR with primers amplifying two informative regions of the genome: a conserved region in the E2 gene and a variable region in the nsP3 gene. The sequences revealed that the strain from Poland belonged to subtype SAV 2, indicating a very strong genetic identity with isolates from Italy and France.

10

Stan zdrowotny akwakultury w Polsce ze szczególnym uwzględnieniem terenów Polski południowo-wschodniej Cz. I. Choroby wirusowe

71%

Matras M. , Stachnik M. , Borzym E. , Maj J. , Reichert M.

Przegląd Rybacki

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2013

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tom 37

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nr 5[131]

11

Filogenetyczna analiza wirusa wiosennej wiremii karpi SVC identyfikowanego na terenie Polski

71%

Maj-Paluch J. , Borzym E. , Matras M. , Stachnik M. , Reichert M.

Medycyna Weterynaryjna

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2019

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tom 75

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nr 05

EN

Spring viremia of carp (SVC) is a disease caused by a virus belonging to the genus Vesiculovirus, family Rhabdoviridae. The SVC virus is divided into four genogroups, Ia, Ib, Ic, and Id, due to its geographical distribution. This study aimed to identify the genotype of the SVC virus circulating in Poland. Polish SVC virus isolates were propagated on EPC and FHM cell lines, and genetic material (RNA) was isolated. The virus was detected in test samples by reverse transcription, sequenced and analyzed using MEGA 6.06 software. The phylogenetic tree was constructed by the Neighbor-Joining method. The results of phylogenetic analysis revealed the presence of two genogroups of the SVC virus in Poland. Most of Polish isolates belonged to the genogroup Id, as do isolates AY196200 from the Czech Republic, Z37505 from Belgium and EF593149 from the United States. Only two Polish isolates from Silesian Voivodeship were more closely related to Chinese and US isolates belonging to the genogroup Ia. There were no isolates belonging to the genogroups Ib and Ic. Nucleotide sequence analysis revealed certain point mutations between particular isolates. Knowledge on the genetic variants of the SVC virus circulating in Poland will be useful in epizootic investigations and preventive measures to protect Polish aquaculture from new variants from the neighboring countries.

12

Choroby wirusowe występujące u ryb ze szczególnym uwzględnieniem karpiowatych

71%

Matras M. , Stachnik M. , Borzym E. , Maj-Paluch J. , Reichert M.

Przegląd Rybacki

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2014

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tom 38

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nr 2[134]

13

Przydatność badań serologicznych w diagnostyce zakaźnej martwicy układu krwiotwórczego ryb łososiowatych (IHN)

61%

Matras M. , Maj J. , Stachnik M. , Borzym E. , Lewandowska A. , Mazur W.

Medycyna Weterynaryjna

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2012

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tom 68

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nr 12

EN

The purpose of the project was to study the correlation between the presence of the IHN virus and the presence of antibodies against this virus in fish sera. Experimental infection of rainbow trout was performed at temperatures of 12°C±1 and 15°C±1. The trouts’ sera were collected at 7-day intervals. Samples from farms naturally infected with IHNV were also tested. The presence of antibodies against IHNV was determined by seroneutralization and the virus was detected by isolation in cell culture. It was found that in the experimentally infected rainbow trout, antibodies against IHN were found earlier in the higher temperature, after 21 days post infection, whereas the presence of IHNV was observed to last longer in the lower temperature. In the study performed on 30 samples of sera from IHN-infected farms, antibodies were detected in 12 samples. Detection of antibodies against IHNV in fish serum can be a helpful tool supporting recommended techniques.

14

Mikroflora i parazytofauna obcych i inwazyjnych gatunków żółwi

61%

Golawska O. , Demkowska-Kutrzeba M. , Borzym E. , Rozanski P. , Zajac M. , Rzezutka A. , Wasyl D.

Postępy Mikrobiologii

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2017

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tom 56

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nr 2