Wyniki wyszukiwania - Biblioteka Nauki

1

Odpowiedź immunologiczna zwierząt zakażonych Trichophyton verrucosum oraz immunizowanych swoistymi szczepionkami. II. Odpowiedź immunologiczna bydła

100%

Ziolkowska G.

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1984

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tom 39

2

Wystepowanie grzybow z rodzaju Malassezia w zewnetrznym kanale sluchowym u psow

63%

Ziolkowska G. , Nowakiewicz A.

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tom 60

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nr 03

310-313

3

Wystepowanie grzybow drozdzopodobnych w stadach gesi reprodukcyjnych

63%

Ziolkowska G. , Tokarzewski S.

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nr 10

1181-1185

4

Choroby grzybicze ptaków

63%

Tokarzewski S. , Ziolkowska G.

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nr 01

5

Wewnatrzgatunkowe roznicowanie szczepow Malassezia pachydermatis z wykorzystaniem PCR-REA

63%

Nowakiewicz A. , Ziolkowska G.

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tom 64

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nr 06

822-827

EN

The aim of the study was to make an attempt at showing the intraspecies heterogenecity of Malassezia pachydermatis strains with regards to their origin (strains isolated from healthy dogs and with otitis externa symptoms). The study included 41 strains of Malassezia pachydermatis species isolated in a pure culture from dogs with clinical otitis externa symptoms (n = 20), clinically healthy dogs (n = 20) and a reference strain, M. pachydermatis (CBS7925). In order to isolate the genetic material from the fungal cells, the following four procedures were selected: mechanical, enzymatic, thermal and chemical. Considering the yield and repeatability of a method for the genomic DNA extraction, a mechanical method was applied. The genetic material research of each strain was performed according to PCR-REA technique with the amplification of three genome regions: ITS, LSU rRNA and a gene encoding beta-tubuline. The ITS and LSU rRNA regions were amplified employing the standard PCR reagents, whereas the region coding beta-tubuline with the so called touch down. The obtained amplification products were subjected to restrictive analysis by means of the following enzymes: EcoRI, Ncol, Hinfl, Alul, and Eco881 (Aval). The performed investigations made it possible to reveal the genotypic differentiation within M.pachydermatis species as well as some correlation between a genotypic profile and the origin of a strain (from healthy animals or with otitis externa symptoms), which may imply the existence of genetic conditioning of the Malassezia strains’ pathogenicity.

6

Izolacja lipidozaleznych szczepow Malassezia od psow i kotow

63%

Ziolkowska G. , Nowakiewicz A.

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tom 62

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nr 08

913-916

EN

The study investigated 180 clinically healthy dogs and 35 cats with symptoms of otitis externa. 96 strains of Malassezia were isolated, including 13.5% (13 strains) of the lipid-dependent species, and the remainder was classified as M. pachydermatis. Ten lipophilic isolates came from diseased animals, two of which were isolated from dogs. M. globosa (5 strains), M. sympodialis (5 strains), M. furfur (one strain) were isolated within the lipophilic strain pool by using phenotype classification and two isolate species remained unidentified. Genotype identification was performed by PCR-REA (ITS, 26S,Bt) and biochemical identification results for all M. sympodialis and M. globosa strains were confirmed. The M. furfur strain and two isolates of an unrecognizable species were reclassified to M. pachydermatis. Isolating lipid-dependent Malassezia strains from animals having otitis externa is a unique phenomena, and, it seems, that this is the first time in which their isolation and identification from dogs by the use of molecular biology techniques has been described.

7

Aktualne problemy w leczeniu i profilaktyce infekcji grzybiczych u ptakow

63%

Ziolkowska G. , Tokarzewski S.

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tom 62

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nr 1

70-79

8

Chlamidospory Trichophyton verrucosum

63%

Wawrzkiewicz K. , Ziolkowska G.

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nr 04

9

An inactivated vaccine against Microsporum canis infection in kittens

51%

Wawrzkiewicz K. , Sadzikowski Z. , Ziolkowska G. , Wawrzkiewicz J.

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tom 36

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nr 1-4

10

Przeciwgrzybowe właściwości wyciągu z owoców arcydzięgla lekarskiego (Archangelica officinalis Hoffm.)

51%

Wawrzkiewicz K. , Wolski T. , Ziolkowska G. , Kawka S.

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tom 46

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nr 08

EN

The purpose of the studies was to determine the anti- iungal properties of the coumarin preparation obtained тот the fruits of angelica against five different species pathogenic fungi. All the tested dermatophytes proved о be most sensitive: MIC ranged from 6.25 to 12.5 meg nl-1. Less sensitive was the Aspergillus niger strain MIC = 25 µg ml-1) and Candida albicans and Pityrospo- um pachydermatitis (MIC=250 µg ml-1). Fungicidal effect MFC) ranged from 25 to over 100 µg ml—1 for derma- ophytes and over 1000 µg ml-1 for other strains tested. Minimal toxic titre of the extract in the cell culture was 50µg ml-1 and was approximately 20 times higher than hat which inhibited the growth of dermatophytes.

11

Test ELISA w ocenie odpowiedzi humoralnej na antygeny Microsporum canis u zwierzat

51%

Ziolkowska G. , Wawrzkiewicz K. , Sadzikowski Z.

Medycyna Weterynaryjna

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1999

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tom 55

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nr 05

306-311

12

Microsporum canis - glowny czynnik etiologiczny grzybicy skory kotow i psow

51%

Wawrzkiewicz K. , Ziolkowska G. , Czajkowska A. , Wawrzkiewicz J.

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tom 50

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nr 07

319-322

EN

The studies were carried out on 71 long-hair cats (mostly Persians), nine short-hair cats (mixed breeds) and 21 dogs of different breeds suspected of ringworm. The samples of epidermis and hair from long-hair cats enabled the isolation of fungi from 80.3 per cent of the animals. Usually M. canis was found (95 per cent) while only sporadically Trichophyton mentagrophytes and Scopulariopsis sp. Out of 13 samples taken from ears, in 6 cases M. canis was isolated, and M. canis together with Malassezia pa- chydermatis in 4 cases. Out of 4 samples from paws M. canis was isolated in 3 cases. In the group of short-hair cats M. canis was noted in 22 per cent of the animals and also in 42.9 per cent of the dogs. Dermatophytosis was found mostly in cats aged 1-6 months (92.1 per cent) and dogs up to 2 years of age (71 per cent). Along with the increase in the age of the animals, the rate of infection decreased. In addition, M. canis was also isolated from 7 owners of cats and owners’ families in which local lesions on the skin were observed.

13

Grzybicze wrzodziejące zapalenie rogówki o złożonej etiologii u konia

51%

Balicki I. , Ziolkowska G. , Smiech A.

Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii

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2011

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nr 4

PL

Opisano przypadek klinicznego grzybicze zapalenie rogówki o złożonej etiologii u konia. Chorobę rozpoznano u klaczy, maści gniadej, w wieku 7 lat. Badanie okulistyczne wykazało w dolnym skroniowym kwadrancie rogówki: wrastanie naczyń krwionośnych, naciek zapalny, obrzęk, wieloogniskowe nacieki podnabłonkowe oraz powierzchowne owrzodzenie rogówki z oddzieleniem nabłonka. Cytologia impresyjna oraz badania mikologiczne wykazały w pobranym materiale obecność Aspergillus fumigatus, a także pojedyncze kolonie Aspergillus flavus. Koń był leczony chirurgiczne oraz przy użyciu kropli okulistycznych z 0,2% flukonazolu i 30% DMSO. Doustnie podawano kapsułki flukonazolu, w dawce 500 mg dwa razy dziennie. Po 2 tygodniach od operacji stwierdzono wygojenie się owrzodzenia rogówki, z obecnością białego koloru blizny. Leczenie farmakologiczne kontynuowano przez 12 tygodni. Badanie kontrolne wykonane po 1 roku wykazało znaczne zmniejszenie blizny w porównaniu do stanu po operacji, brak naczyń krwionośnych i w miejscu wrzodu zwiększenie przejrzystości rogówki.

EN

The authors report a clinical case of poliethiological ulcerative mycotic keratitis in horse. The disease was diagnosed with mare, bay colour, aged 7 years. The ocular examination revealed in the inferior cranial quadrant of the right cornea: the superficial and deep blood vessel ingrowth, oedema, multifocal dark brown subepithelial infiltrations as well as superficial corneal ulceration with the demarcated epithelial. The impression cytology and mycological evaluation showed the presence of Aspergillus fumigatus as well as single colonies of Aspergillus flavus in the examined research material. The horse was surgery treated and with the use ophthalmic drops containing 0,2% fluconazole and 30% DMSO mixture. Oral fluconazole 500mg was given twice a day. The ophthalmic examination revealed the complete recovery of the corneal ulceration, with a white scar 2 weeks after operation. The pharmacological treatment was continued by 12 weeks. The control check-up a year after the treatment institution showed no blood vessels growing into the cornea, marked diminishment of the postoperative scar as well as higher corneal transparency at the ulcer site.

14

The path of Microsporum canis invasion in the Guinea Pig, clinical, mycological and histopatological studies

51%

Wawrzkiewicz K. , Cybulska R. , Ziolkowska G. , Wawrzkiewicz J.

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tom 48

1-16

15

Mikrobiota grzybicza powlok zewnetrznych zwierzat wolno zyjacych

51%

Ziolkowska G. , Budziwojska M. , Nowakiewicz A. , Zieba P.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 10

719-722

EN

The objective of the research was to assess the presence of mycotic microbiota on the integument of wild boars and roe-deer, as well as to isolate and identify each species. The research material comprised groin screening swabs collected from 13 wild boars and 56 roe-deer from the Lublin State Forests. The fungi were identified concurrently on the Sabouraud and MLNA medium at 25°C, 32°C and 37°C temperature for 14 days. Initial identification proceeded according to the conventional mycological procedures followed by the application of the commercial API Candida and API 20C Aux (bioMerieux) (Candida genus) tests and the phenotypic scheme developed by Guillot et al. (Malassezia genus). The present research has revealed that mycotic flora was recovered in all a total of 69 examined animals. The most frequently isolated fungi included Penicillium spp., Alternaria spp., Cladosporium spp. and Malassezia spp. and Rhodotorula spp. The species analysis of the isolated fungi has confirmed the presence of potential pathogens, such as Malassezia sympodialis, Aspergillus fumigatus and Candida non-albicans. The obtained results indicate that a population of free-living animals may constitute a critical link in the epidemiologic chain of mycotic infections.

16

Inaktywowana szczepionka przeciwko grzybicy skornej kotow wywolywanej przez Microsporum canis

51%

Wawrzkiewicz K. , Sadzikowski Z. , Ziolkowska G. , Wawrzkiewicz J.

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nr 04

245-250

EN

The purpose of the work was to determine the protective properties of a vaccine, called Felisvac Me, developed according to the authors’ own technology. Examinations were carried out on 33 domestic, shorthaired cats 1-6 months of age. The degree of specific immunity was evaluated by a challenge trial, contact infection (simulation of natural contagion), delayed type of hypersensitivity test and the migration inhibition test of leukocytes. It was found that the vaccine without an adjuvant in a dose of 2 ml did not fully protect one-month old cats from being infected with a virulent strain of M. canis. Cats, which were given a higher dose of the vaccine (3 ml per animal) adsorbed on aluminium hydroxide, turned out to be immune to both methods of infection by challenge trials. The vaccine elicited a delayed type of hypersensitivity that persisted on almost a similar level (0.8--1.1 mm) throughout the observation period, i.e. 5 months. Positive values (25.2-32.4%) of the leukocyte migration inhibition test - the consecutive determinant of cellular type of immunity, were found between 22 and 41 days after vaccination. The above vaccine is characterised by a high protective value; it is also safe for both animals and environment. No side effects have been observed.

17

Immune response of cats experimentally infected with Microsporum canis

51%

Wawrzkiewicz K. , Ziolkowska G. , Sadzikowski Z. , Stepien M.

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nr 2

EN

The aim of this study was an assessment of the immune response of cats experimentally infected with M. canis and a determination of the extent of immunity acquired after recovery. Studies were carried out on 24 domestic, short-haired cats, aged 3-4 months. The resistance of the animals was assessed by reinfection, and the degree of immune response. It has been found that after recovery from an experimental infection with M. canis, the cats which remained in an infected environment for 18 weeks showed no clinical mycotic changes, whereas control animals underwent natural infection within 6 weeks and their mycosis had a typical course. Furthermore reinfection with high doses of the fungus and skin scarification failed to break down this acquired immunity. Hypersensitivity of the delayed type, encountered in cats within 3 weeks after the experimental infection, persisted for 8 months of studies. In an analogous period of time cats infected naturally demonstrated an evidently weaker response determined by the skin test. Positive results of the migration inhibition test were obtained in the period of 3-6 weeks after infection, which corresponded with the period of the persistence of the clinical mycotic changes.

18

An evaluation of the resistance to Microsporum canis on the basis of the guinea pig

51%

Wawrzkiewicz K. , Ziolkowska G. , Wawrzkiewicz J.

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nr 3-4

19

Effect of balanced supplementary feeding in winter on qualitative and quantitative changes in the population of microbes colonizing the rumen of red deer

32%

Gnat S. , Dziedzic R. , Nowakiewicz A. , Zieba P. , Troscianczyk A. , Majer-Dziedzic B. , Ziolkowska G. , Beeger S. , Wojcik M.

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nr 02

EN

Ruminants are a group of animals that process and assimilate their food in a unique manner. The functioning of the digestive tract of these animals is closely related to the abundance and composition of microbes in the forestomach, which is a complex ecosystem of bacteria, protozoa and fungi. Microorganisms present in the rumen, and in particular their effect on physiological processes in the body, influence the animal’s physical condition and state of health. Microbiological examination of rumen microbiota ecology is hindered by a lack of selective growth media, as well as by difficulties in isolating bacteria in vitro and accurately identifying them. The aim of the study was to evaluate the effect of food consumed by red deer (Cervus elaphus) on the diversity of their rumen microbiota. Microbes were compared in two study periods. In autumn the animals’ diet came exclusively from natural plant sources, while in winter, supplementary feeding was introduced, including specially prepared fodder. The study showed that in deer that did not receive the special fodder in winter, but only natural plant components, the abundance of bacterial flora decreased significantly compared with what it was in autumn, unlike in animals that did receive the fodder, whose composition and caloric value substantially increased the activity of rumen microbes. In winter, changes in proportions of different morphological forms of rumen bacteria were observed, as well as a decline in their total number, particularly in the animals that did not receive the pellets. A similar decline was also observed in the populations of yeasts and protozoa in winter. To sum up the results of the study, the use of the specially prepared high-calorie fodder in winter was shown to influence the rumen ecosystem of red deer. The most significant factor improving the condition of deer receiving supplementary fodder during this period is the stabilization of bacterial flora in the rumen, which directly contributes to the efficiency of digestion.