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A biomechanical comparison between tissue stiffness meter and shore type 00 durometer using fresh human fetal membrane cadavers

100%

Oflaz H.

tom Vol. 36, no. 1

138--144

A manual palpation is traditionally used on soft tissue stiffness evaluation in clinical practices. However, the palpation is a subjective technique, so quantitative tissue stiffness measurement would be a more reliable method on diagnosing disorders instead of a palpation in medicine. The purpose of this study was to emphasize a new medical device that was capable of measuring soft tissue stiffness. An in vitro investigation with a soft tissue stiffness meter (STSM) was presented and it is compared with a shore type 00 durometer in this study. Soft materials were needed for in vitro experiments to show feasibility of the STSM, so fetal membranes were decided to use on experiments. Five fetal membranes undergoing normal birth (NB) (35 samples, 105 measurements) and four fetal membranes undergoing pre-term birth (PRB) (20 samples, 60 measurements) were collected immediately after delivery. Samples were examined on custom designed tissue holder. Resu*lts of the STSM were in correlation with results of the durometer for NB and PRB (r2 = 0.995 and r2 = 0.996 respectively). Moreover, a tissue stiffness difference between NB and PRB was statistically significant by using STSM ( p ≤ 0.001), whereas it was not statistically significant by using durometer ( p = 0.360). In conclusion, newly produced device, STSM is more sensitive than durometer even for very small stiffness differences as between NB and PRB fetal membranes.

The effect of zinc oxide doping on mechanical and biological properties of 3D printed calcium sulfate based scaffolds

51%

Aldemir Dikici B. , Dikici S. , Karaman O. , Oflaz H.

tom Vol. 37, no. 4

733--741

Fabrication of defect-matching scaffolds is the most critical step in bone tissue engineering. Three-dimensional (3D) printing is a promising technique for custom design scaffold fabrication due to the high controllability and design independency. The objective of this study is to investigate the effect of zinc oxide (ZnO) doping on mechanical and biological characteristics of 3D printed (3DP) calcium sulfate hemihydrate (CSHH) scaffolds. Crystalline phases, wettability, compressive strength and Young's modulus, human bone marrow derived mesenchymal stem cells (hMSCs) attachment, proliferation and morphology were investigated. XRD results showed that CSHH powder transformed into gypsum after the printing process due to the water content of binder. Contact angle measurements indicated that ZnO doped CSHH scaffolds have hydrophilic character, which stimulates cell attachment. The mechanical and cell culture studies demonstrated that increasing the ZnO doping concentration both mechanical strength and cell proliferation on CSHH scaffolds were enhanced.