Wyniki wyszukiwania - Biblioteka Nauki

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Immunohistochemiczna ocena wpływu rebaudiozydu A na neurony zawierające acetylocholinoesterazę w hipokampie i w prążkowiu szczurów

100%

Rycerz K. , Jaworska-Adamu J.

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nr 10

EN

The aim of the study was to investigate acetylcholinesterase-immunoreactive neurons in the CA1 area of the hippocampus and in the striatum (CS) of rats receiving rebaudioside A (RebA) for 15 days. RebA is a steviol glycoside used in the production of sweeteners, and it has been shown that glycosides affect memory and learning processes. RebA was administrated to adult rats for 15 days at 1 mg of glycoside/ml of water (group I) and 2 mg of glycoside/ml of water (group II). An indirect immunohistochemical peroxidase-antiperoxidase reaction was performed on frontal slides containing the hippocampus and CS with the use of a monoclonal antibody against AChE. Neurons immunoreactive for the protein were assessed morphologically and morphometrically in hippocampal area CA1 and in the CS. Microscopical observations did not reveal significant morphological changes in immunopositive neurons, which suggests that the glycoside had no neurotoxic effect of these cells. Morphometric analyses did not show changes in the density of AChE-immunoreactive neurons. On the other hand, a decrease in reaction intensity was demonstrated in hippocampal area CA1 in group I and in the CS in both groups of animals receiving RebA. The results of our preliminary studies suggest that RebA affects cholinergic neurons.

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Age-related astrocytic changes in the periaqueductal gray matter (PAG) in rats

80%

Jaworska-Adamu J. , Krawczyk A. , Rycerz K. , Krawczyk-Marc I.

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nr 10

EN

Astrocytes are glial cells prone to morphological changes associated with age. The aim of the study was to investigate the immunoreactivity of glial fibrillary acidic protein (GFAP) in astrocytes of the periaqueductal gray matter (PAG) of the midbrain in adult and old male rats to demonstrate morphological changes associated with age and to assess morphometrically the number of astrocytes and the digital immunostaining intensity of the examined protein in PAG astrocytes of both groups of animals. In the study, 10 male Wistar rats in two age groups were used. The first group consisted of five 100-day-old animals, whereas the second comprised five 3-year-old rats. After euthanasia, the midbrain, containing PAG, was collected and embedded in paraffin blocks. Immnunohistochemical peroxidase-antiperoxidase reaction was carried out on coronal tissue sections with the use of the specific primary antibody against GFAP, goat anti-mouse IgG, peroxidase-antiperoxidase complex, and diaminobenzidine chromogen. GFAP-immunopositive PAG astrocytes were observed under a light microscope and subjected to morphometric analysis to determine their number and digital immunostaining intensity for the protein examined. GFAP-immunoreactive PAG astrocytes in 100-day-old rats showed uniform distribution. Numerous processes branching into secondary ones protruded from intensely GFAP-immunostained stellate cells. In contrast, in 3-year-old rats a significantly lower number of glial cells of different morphology was observed compared to young animals. Astrocytes had fewer primary processes without secondary branches. Morphometric analysis confirmed microscopic observations. Our findings indicate that PAG astrocytes are prone to quantitative and morphological changes with age, which, in turn, can cause disorders in emotional, pain, and defensive reactions.

3

CrossGrid - tools and services for interactive grid applications

80%

Bubak M. , Malawski M. , Rycerz K. , Turała M.

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tom Vol. 8, No 4

503-512

EN

The CrossGrid Project aims to develop new Grid services and a tool environment for a representative set of interactive compute- and data-intensive applications. This paper presents the architecture of the software and a description of the functionality of the main components. The new tools and grid services are based on the Globus Toolkit and the EU DataGrid middleware. CrossGrid develops and operates a large international testbed, where the developed applications, services and tools are deployed and run.

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Interactive Grid computing: adapting high level architecture-based applications to the Grid

80%

Rycerz K. , Bubak M. , Malawski M. , Sloot P.

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tom Vol. 8, No 4

549-559

EN

In this paper we present the design of a Grid HLA Management System (GHMS) supporting execution of High Level Architecture-based interactive applications in a Grid environment, while at the same time allowing for running HLA legacy codes to preserve backward compatibility with the HLA standard. In particular, we describe the following system components: an HLA-Speaking Service for multiple federates that interface the HLA application to the system, a Monitoring Service integrated with the OCMG monitoring system and HLA-based Benchmark Services informing the Broker Service of what can be expected from the application's behavior.

5

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

80%

Rycerz K. , Krawczyk A. , Jaworska-Adamu J. , Krawczyk-Marc I.

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nr 4

6

Immunoreactivity of the calbindin D28k in the parahippocampal gyrus of chinchilla

71%

Szalak R. , Jaworska-Adamu J. , Rycerz K. , Kulik P. , Arciszewski M. B.

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tom 57

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nr 3

EN

Ten adult male chinchillas were used. The localisation of calbindin D28k (CB) was examined with the use of two types of reactions: immunocytochemical peroxidase-antiperoxidase and immunofluorescence staining with a specific monoclonal antibody against CB. Immunocytochemical examination demonstrated the presence of CB-positive neurons in the following layers of all parts the parahippocampal gyrus (PG): marginal, external cellular, middle cellular, and internal cellular, i.e. in entorhinal area, parasubiculum, and presubiculum. Immunofluorescence staining revealed the presence of CB in both Hu C/D- immunoreactive (IR) neurons and nervous fibers of the PG. CB-IR neuronal cell bodies were moderately numerous (ca. 10% of Hu C/D-IR neurons) and clearly distinguished from the background. Each layer of the brain area consisted of two types of neurons: pyramidal and multiform. Among the second type of neurons, four kinds of morphologically different neuronal subclasses were observed: multipolar, bipolar, round, and Cajal-Retzius cells. It is concluded that the expression of CB in the PG of the chinchilla is species specific and limited to several subclasses of neurons.

7

Immunoreactivity for calretininin interneurons of the hippocampal CA1 field and dentate gyrus in adult rats after administration of habanero peppers (Capsicum chinense Jacq.)

61%

Jaworska-Adamu J. , Krawczyk A. , Rycerz K. , Gołyński M. , Wawrzyniak A. , Lutnicki K. , Balicki I.

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8

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

61%

Rycerz K. , Krawczyk A. , Jaworska-Adamu J. , Szalak R. , Tomaszewska E. , Dobrowolski P.

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nr 06

EN

The aim of the study was to investigate the morphology, density and immunostaining intensity of calbindin (CB)-positive neurons of dentate gyrus (DG) in new-born (P0) and 21-day-old (P21) male Acomys cahirinus mice from dams receiving β-hydroxy-β-methylbutyrate (HMB) during pregnancy. Different substances administrated to pregnant dams may affect the calcium ion homeostasis which is crucial for the proper brain development of their offspring. DG with hilus (H) plays an important role in memory and learning processes. Calcium levels in DG are regulated by buffering proteins like calbindin D28k (CB). Experimental dams were orally treated with HMB at a dose of 0.2 g/kg b.w. Half of new-born animals were euthanised after birth and the rest after the 21st day of life. The brains were dissected and embedded in paraffin blocks using a routine histological technique. In order to demonstrate CB protein expression an immunohistochemical peroxidase-antiperoxidase reaction was conducted. The results of the study did not reveal important morphological alterations. There were no statistically significant changes in the density of the studied cells either in P0 and P21 animals. However, the authors have demonstrated a statistically significant increase of the average CB-immunostaining intensity in nuclei and cytoplasm in both age groups. It may be a result of a compensation effect to alterations that occurred under the influence of HMB. On the basis of the conducted research, it may be assumed that HMB activity in DG may provide long-term consequences.

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Colocalization pattern of cocaineand amphetamine-regulated transcript peptide and parvalbumin immunoreactivity in the hippocampus proper of the chinchilla

61%

Szalak R. , Matysek M. , Kukula-Koch W. , Rycerz K. , Zacharko-Siembida A. , Arciszewski M. B.

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tom 23

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nr 1

10

Colocalization pattern of cocaine- and amphetamine-regulated transcript peptide and parvalbumin immunoreactivity in the hippocampus proper of the chinchilla

57%

Szalak R. , Matysek M. , Kukula-Koch W. , Rycerz K. , Zacharko-Siembida A. , Arciszewski M. B. , Szalak R. , Matysek M. , Kukula-Koch W. , Rycerz K. , Zacharko-Siembida A. , Arciszewski M. B.

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