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Human tuberculosis in colonial Victorian and present-day Britain

100%

Cooper R. G.

nr 1

Expression profile of MYF5 and MYF6 genes in skeletal muscles of young growing gilts of five breeds at different ages, based on the most stable reference genes

32%

Pierzchala M. , Pareek C. S. , Lisowski P. , Urbanski P. , Goluch D. , Czarnik U. , Kamyczek M. , Rozycki M. , Cooper R. G. , Kuryl J.

nr 3

The expression profile was evaluated of MYF5 and MYF6 genes in skeletal muscles of young growing Polish Large White (PLW), Polish Landrace (PL), Pietrain (PIE), Duroc (DUR) and Pulawska (PUL) gilts at different ages. Normalization of MYF5 and MYF6 was performed on reliable porcine reference genes (PRGs), where expression stabilities of nine of them (ACTB, B2M, GAPDH, SDHA, HPRTI, RPL13A, YWHAZ, TBP, TOP2B) were evaluated by RT-qPCR method and NormFinder software. Results revealed HPRTI, TBP and TOP2B as highly stable and PRGs. The age-dependent and breed-specific skeletal muscle expression comparisons revealed highly significant (P<0.01) differences in MYF6 expression levels of all skeletal muscles among investigated breeds. MYF6 gene expression in PIE and DUR were higher compared to PLW, PL and PUL gilts. Contrarily, paired-wise comparison of MYF5 gene expression showed only significant difference between DUR and PUL for semimembranosus, and PL and PLW, DUR and PL, PIE and PL, DUR and PUL and PIE and PUL for gluteus medius muscle. There was no significant relationship identified between gilt ages and the level of expression of MYF5 and MYF6 genes. However, their highest expression was identified in longissimus dorsi followed by gluteus medius and semimembranosus muscles. It is concluded that normalization of gene expression has to be done on more than one PRG to reduce the errors in transcription level estimates. Moreover, significantly different breed-specific expression of porcine MYF5 and MYF6 allowed the authors to prioritize these genes as potential candidate genes for trait-associated study.

Cholesterol content and fatty acid composition of two fat depots from slaughter ostriches [Struthio camelus] aged 14 months

32%

Horbanczuk J. O. , Malecki I. , Cooper R. G. , Jozwik A. , Klewiec J. , Krzyzewski J. , Khalifa H. , Chylinski W. , Wojcik A. , Kawka M.

nr 2

The cholesterol content differed (P<0.05) between breast (49.5 mg/100 g) and back (74.3 mg/100 g) fat. Differences (P<0.05) in individual fatty acids were found especially for arachidonic acid (20:4).High contents of 18:2, 18:3 and 20:4 from both depots suggest, that ostrich fat could be a source of essential fatty acids in human and animal diets. The influence of various factors and especially feeding regimen on quantity and quality of ostrich fat should further be investigated.

Pod względem poziomu cholesterolu tłuszcz z okolicy mostka różnił się od tłuszczu pochodzącego z grzbietu (odpowiednio 49,5 i 74,5 mg/100 g, P<0,05). W zawartości kwasu arachidonowego (20:4) wystąpiła różnica (P<0,01) na korzyść tłuszczu grzbietowego. Wysoka zawartość 18:2, 18:3 i 20:4 w tłuszczu z obu okolic wskazuje, że tłuszcz podskórny strusi może być źródłem niezbędnych kwasów tłuszczowych w diecie ludzi i zwierząt. Potrzebne są dalsze badania wpływu czynników środowiskowych, a zwłaszcza żywienia na ilość i jakość tłuszczu pozyskiwanego od strusi rzeźnych.