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EN
Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods.Three Huai and three Landrace pigs each were individually housed at the Fujian Shanghang Huai pig breeding farm under similar feeding conditions. Pigs were humanely slaughtered at saleable weight and samples of kidney, liver and skeletal muscle were collected for expression analyses.HMGR mRNA expressions in liver, muscle and kidney of Huai pigs both occurred higher than expression found in the Landrace. Positive staining of HMGR protein was revealed in the three tissues considered. The HMGR protein expression level was not significant statistically in liver,muscle and kidney across two breeds, but the amount of Huai pig HMGR protein expressed in three tissues was higher than that of the Landrace. The level of HMGR protein expression in liver was higher than in the muscle and renal tissues (P<0.05). The results demonstrate that HMGR expression in pigs may depend on tissue and species.
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EN
Somatic hybridization by protoplast fusion from cell suspension cultures and leaf parent has been a well-established technique holding great potential for citrus variety improvement. In this study, somatic hybrid plants were regenerated from the following two fusion combinations: ‘Murcott’ tangor (Citrus reticulata Blanco × C. sinensis (L.) Osbeck) + Hirado Buntan Pink pummelo (HBP) (C. grandis (L.) Osbeck) and ‘Bingtang’ orange (C. sinensis (L.) Osbeck) + Calamondin (C. microcarpa Bunge). Somatic hybrids were selected at an early stage based on their higher capacity for embryogenesis comparing to non-hybrid cells. Flow cytometry analysis showed that all plants from pre-selected lines of the two combinations were tetraploid. SSR analysis confirmed their hybrid nature, with nuclear DNA from both fusion parents, and absence of parental specific bands was also detected. Cytoplasmic compositions of the recovered plants were further revealed by CAPS and cpSSR analysis. The allotetraploid somatic hybrids from the ‘Murcott’ tangor + HBP combination will be applied to develop triploid seedless cultivars by interploid crossing with diploid seedy citrus cultivars, and those from ‘Bingtang’ orange + Calamondin could be valuable for Asiatic citrus canker-tolerant and ornamental citrus breeding.
EN
Industrial Radiography is widely used in non-destructive testing (NDT ) in the world. Workers in the sector exposure if incidents happen. The Information System on Occupational Exposure in Medicine, Industry and Research: Industrial Radiography so called ISE MIR-IR was developed by the IAEA as a web-based tool for a regular data collection and analysis of occupational exposure. The system was launched with full function in 2017. It was designed based on the data from an extensive research and results of the worldwide surveys. It assists NDT companies to benchmark their own companies and individual performance against others in the database. NDT companies all around the world are encouraged to participate in the database to enable it to become a worldwide tool for implementing optimization of occupational radiation protection.
PL
Radiografia przemysłowa jest szeroko stosowana w badaniach nieniszczących (NDT ) na świecie. Pracownicy tego sektora w przypadku wystąpienia incydentów mogą być narażeni na niebezpieczeństwo. System Informacji o narażeniu zawodowym w medycynie, przemyśle i badaniach: Radiografia przemysłowa (ang. The Information System on Occupational Exposure in Medicine, Industry and Research: Industrial Radiography) nazwany ISE MIR-IR został opracowany przez IAEA jako internetowe narzędzie do regularnego gromadzenia danych i analizy narażenia zawodowego. System został uruchomiony z pełną funkcjonalnością w 2017 roku. Zaprojektowano go w oparciu o dane pochodzące z obszernych badań i wyników światowych sondaży. Pomaga firmom z branży NDT w porównywaniu własnych i indywidualnych wyników z innymi wynikami w bazie danych. Firmy NDT na całym świecie są zachęcane do uczestnictwa w tworzeniu bazy danych, aby umożliwić jej stanie się światowym narzędziem do wdrażania optymalizacji ochrony przed promieniowaniem zawodowym.
EN
A tiny and low-cost ground-based Fabry–Perot interferometer (FPI) was designed using a filter behind etalon and Galilean telescope system for the thermospheric wind observation with OI 630.0 nm nightglow emissions (~250 km). Based on the instrument, experiments were carried out at Langfang (39.40° N, 116.65° E) site for a rough comparison and Kelan (38.71° N, 111.58° E) site for a detailed validation. Wind results of Langfang experiment are well consistent with measurements of two other FPIs deployed at Xinglong (40.40° N, 117.59° E) and Kelan which are retrieved by the American National Center for Atmospheric Research (A-NCAR). In Kelan experiment, the averaged wind deviation between our FPI and A-NCAR FPI is 11.8 m/s. The averaged deviation of wind measurement error between them is 2.9 m/s. The comparisons suggest good agreement. Then, the analysis of influencing factors was made. The center determination offset has an exponential relation with wind deviation, while the radius calculation offset is linear with wind deviation.
EN
The paper evaluates effectiveness of fire drills for emergency and responding to PSC inspections on board. A brief background about the PSC inspection on fire drills on board is introduced in the beginning. Then the significance of effectiveness evaluation on fire drills is presented. Next, legal ground is discussed on International Conventions, including regulation of related regional group, national maritime laws and rules and Concentrated Inspection Campaign (CIC). Furthermore, PSC New Inspection Regime (NIR) for Paris MOU and Tokyo MOU are also discussed, and many deficiencies related to fire safety measures found in the PSC inspection are statistically analyzed. More importantly, the paper introduces System Engineering Theory, presents the principle and method of effectiveness evaluation, focuses on the preparation, performance and rehabilitation of fire drill and develops the Criterion of Effectiveness Evaluation. Finally, some suggestions are raised to carry out effectiveness evaluation for emergency and responding to the PSC inspection.
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EN
A Simple modelling method to identify the kinematic errors of machine tool table is proposed. According to the structure of the machine tool, based on rigid body kinematics, the motion coordinates of major components of the machine tool table was reasonable set up, and the error propagation of the machine tool rotary table was described. The mathematical expression of this model is simple, and the physical meaning is understandable. It is a theoretical foundationon for further error recognition and error compensation.
PL
W artykule przedstawiono metodę rozpoznania błędów kinematycznych stołu do obrabiarki. Wyznaczono funkcje ruchu najważniejszych elementów oraz opisano sposób dalszej propagacji powstałego uchybu. Na tej podstawie możliwe było rozpoznanie błędu i jego kompensacja.
10
Content available remote Optimal supervisory control of regular languages
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EN
This paper presents an algorithm for optimal control of regular languages, realized as deterministic finite state automata (DFSA), with (possible) penalty on event disabling. A signed real measure quantifies the behavior of controlled sublanguages based on a state transition cost matrix and a characteristic vector as reported in an earlier publication. The performance index for the proposed optimal policy is obtained by combining the measure of the supervised plant language with the cost of disabled controllable event(s). Synthesis of this optimal control policy requires at most n iterations, where n is the number of states of the DFSA model generated from the unsupervised regular language. The computational complexity of the optimal control synthesis is polynomial in n. The control algorithms are illustrated with an application example of a twin-engine surveillance aircraft.
EN
QTL mapping for plant-height traits has not been hitherto reported in high-oil maize. A high-oil maize inbred ‘GY220’ was crossed with two dent maize inbreds (‘8984’ and ‘ 8622’) to generate two connected F₂:₃ populations. Four plant-height traits were evaluated in 284 and 265 F₂:₃ families. Single-trait QTL mapping and multiple-trait joint QTL mapping was used to detect QTLs for the traits and the genetic relationship between plant height (PH) and two other plant-height traits. A total of 28 QTLs and 12 pairs of digenic interactions among detected QTLs for four traits were detected in the two F F₂:₃ families. Only one marker was shared between the two populations. Joint analysis of PH with ear height (EH) and PH with top height (TH) detected 32 additional QTLs. Our results showed that QTL detection for PH was dependent on the genetic background of dent corn inbreds. Multiple-trait joint QTL analysis could increase the number of detected QTLs.
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Content available remote Bioprocessing of Bamboo Materials
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EN
Bamboo culms were processed using microorganisms, and fibre bundles were obtained. Bacteria and fungi with xylanase activity were isolated from the bamboo retting system. Chemical composition analysis of the fibre bundles obtained showed that the components are mainly cellulose, hemicelluloses and lignin. An increase in cellulose and hemicelluloses content was detected along with a decrease in lignin content after bio-processing. Environmental Scanning Electronic Microcopy of the fibre bundles (retted) showed great fibre sensibility towards moisture, which could significantly influence mechanical properties. Our results suggested that the bio-processing presented herein contributes to the possible development of a new means of bamboo bio-processing that can be regarded as a primary process to separate fibre bundles from non-cellulosic tissue in the culm.
PL
Łodygi bambusowe przetwarzano przy użyciu mikroorganizmów, uzyskując wiązki włókien. Bakterie i grzyby o aktywności ksylanazy zostały wyizolowane w systemie roszenia. Analiza chemiczna otrzymanych wiązek włókien wykazała, że głównymi składnikami włókien są: celuloza, hemicelulozy i ligniny. Po biologicznej obróbce stwierdzono wzrost zawartości celulozy i hemiceluloz, a spadek zawartości lignin. Opracowany sposób obróbki łodyg bambusowych wykazał, że jest skuteczny i może być rekomendowany do powszechnego stosowania.
EN
The mineralogical and chemical characteristics of heavy metals in tailings and soils is an imperative for potential ecological risk assessment of metals to environment and heavy metals pollution prevention and control. The lead-zinc tailing and contaminated soil in and near the tailing pond were sampeled from a mine tailing pond in Hunan province (China), in which the chemical composition, phase composition and thermal behavior of tailing and soil were investigated. Furthermore, the petrography of lead-zinc tailing and chemical fractionations of Pb and Zn in the contaminated soil were studied in details. The mineral phases of lead-zinc tailing were galena, pyrite, chalcopyrite, sphalerite, quartz and fluorite, as distinguished by the reflected light microscopy and further proofed by the scanning electron microscope-energy dispersive spectrometer under the back scattered electron mode. Chemical fractionations were carried out by the European Community Bureau of Reference (BCR) sequential extraction procedure for Pb and Zn in the soil and the mild acido-soluble (F1), reducible (F2), oxidizable (F3), and residual (F4) fractions were 5.90, 75.24, 4.90 and 13.96% for Pb, and 47.74, 34.06, 9.59 and 8.61% for Zn, respectively. Subsequently, the individual contamination factor (ICF) of Pb and Zn were calculated as 6.16 and 10.61, respectively. The DTPA-available content of Pb and Zn in the contaminated soil were 39.9 and 170.7 mg·kg-1, respectively. The study provided a base for selecting remediation strategies in the studied area.
EN
In this study, the modification mechanism and growth process of Al3(Sc, Zr) particles in as-cast Al-Si-Mg-Cu based alloy with addition of Sc and Zr were systematically investigated. It was found that 0.57 wt-%Sc addition caused a significant refinement in the average grain size of the investigated alloy, which brought about a remarkable transformation in as-cast microstructure, from thick dendritic shape to fine equiaxed structure. A large amount of primary Al3(Sc, Zr) particles with the dimension of around 5-6 μm were also observed within the equiaxed grain. Due to the identical orientation and similar crystal structure between primary Al3(Sc, Zr) particles and α-Al matrix, the primary particles always served as heterogeneous nucleus for the α-Al matrix. In addition, these cusped cubic primary Al3(Sc, Zr) particles showed triangle, star, rhomboid morphologies are generated from sectioning the particle in (111), (100) and (110) planes, respectively. Particularly, the typical eutectic structure which contained odd number-layer (Al3(Sc, Zr)+α-Al+ ... +Al3(Sc, Zr)) was observed within the investigated particles.
EN
We studied the removal of K-2BP from aqueous solution by cetyltrimethylammonium chloride-modified bentonite with polyacrylamide flocculant (CTAC-bentonite/PAM). The preliminary results disclose that the CTAC modification method can potentially enhance the removal efficency of bentonite and, by combination with PAM, the adsorbed bentonite can settle within a short sedimentation time. In addition, alkaline condition is favorable for the adsorption; however, temperature influence is weak. Considering the high efficiency and low cost, CTAC-bentonite/PAM could broadly extend in the treatments of dye wastewater.
EN
We aim to investigate the changes of The y-aminobutyric acid (GABA) signals in the adjacent intact dorsal root ganglion (DRG) and the contribution of these changes to the development and maintenance of neuropathic pain (NPP). After establishment of neuropathic pain model with the lumbar 5 spinal nerve ligation (L5 SNL), the GABA-evoked currents were recorded in the acutely dissociated L4 DRG neurons using whole-cell patch clamp. Moreover, Muscimol or Bicuculline were respectively topically injected to the L4 DRG at the time of nerve injury and post-operative 5 days (POD5). The pain thresholds of hind paw were recorded. We found that the incidence and amplitude of GABA currents significantly decreased in the large and medium L4 DRG neurons after SNL (P<0.05). Furthermore, in the L5 SNL rats, 20 ug Muscimol injected immediately after injury caused a long-lasting attenuation of mechanical allodynia (P<0.05), whereas the thermal hyperalgesia was not alleviated (P>0.05). 0.15 ug Bicuculline further decreased the pain thresholds (P<0.05) and reversed the pain-alleviating effects of Muscimol. However, at POD5, 20 ug Muscimol only exhibited transient alleviating effects (P<0.05). In conclusion, the decrease of inhibition signal mediated by GABA-A receptor in the adjacent intact DRG neurons may be crucial in the development of hyperalgesia in NPP.
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