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1 Acta Physiologiae Plantarum

1 2010

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Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar)

100%

Agrawal A. , Sanayaima R. , Tandon R. , Tyagi R. K.

nr 4

To decrease the cost of in vitro conservation of banana cv. Karpura Chakkarakeli (AAB; Mysore sub-group) without any adverse effects on cultures, expensive components of medium such as sucrose and gelling agents, i.e. phytagel or agar (90% of the total cost of the medium), were replaced with inexpensive alternates such as market sugar and isabgol, respectively (Experiment 1). In general, no significant effects of isabgol and market sugar were observed on shoot (1.0–1.3 shoots/shoot explant) and root (1.5–2.0 roots/shoot explant) regeneration. Up to 12 months, 100% of cultures survived on isabgol-media, which was significantly higher than that on agar-media (79–83%) and on phytagel-media (51–57%). Isabgol-media with or without other constituents of medium were also tested for survival of banana cultures (Experiment 2); significant differences were observed for survival of cultures (20–100%). Slow growth of the cultures on isabgol-media was attributed to low availability of free water and consequently slower rate of transport of nutrients from isabgol matrix to the plantlets than that of other media tested, as evidenced by significantly lower relative matric potentials (0.801 and 0.804) of isabgol-media. In vitro conservation-derived plants grown in the field exhibited no significant morphological variations. The total cost of medium used for in vitro conservation of banana was decreased by 59% by using isabgol as an alternate gelling agent to agar and phytagel.