Wyniki wyszukiwania - Biblioteka Nauki

1

Development of a rapid immunochromatographic assay for detection of antibodies against porcine epidemic diarrhea virus

100%

Li R. , Ma J. , Tian X. , Yu Y. , Qiao S. , Wang Z.

|

nr 1

2

Effects of hole-perpendicularity error on joint stiffness of single-lap double-bolt composite joints

100%

Liu X. , Yang Y. , Gao H. , Bao Y. , Li R. , Chen L.

|

tom Vol. 65, iss. 2

289--305

EN

To investigate the influence of hole-perpendicularity error on stiffness of single-lap doublebolt composite joints, a finite element model was first created and validated by using the analogical mass-spring based model proposed by McCarthy et al. The model was then modified by introducing hole-perpendicularity error, with which the influences of hole-perpendicularity error, which is represented by hole-titling angle, hole-tilting direction, and bolt torque on the joint stiffness are studied. It is found that the hole-tilting direction causes the joint stiffness to either increase or decrease, which depends on the relation between the hole-tilting direction and the loading orientation. In addition, the hole-tilting angle strengthens the influence of hole-tilting direction and the bolt torque plays the most important role among the three factors in affecting the joint stiffness.

3

An Online Monitoring System of Corona Loss of High Voltage Transmission Lines Based on Metalized Membrane Method

84%

Luo Y. , Liu H. , Wang M. , Xin X. , Zhang Y. , Li R. , Wang G. , Wu J. , Bai X.

|

2013

|

tom R. 89, nr 10

217-220

EN

An online monitoring system of corona loss of high voltage transmission lines based on metalized membrane method is developed in this paper. The system includes 3 components: the upper computer, the conductor voltage acquisition module and the corona leakage current acquisition module. The corona leakage current acquisition module is set on the high voltage line. The transmission line is covered by a certain length of thin metalized membrane . The sampling resistance is put paralleling between metal layer and bundle conductor. The conductor voltage acquisition module is set in transformer substation. The two modules are synchronized by the rising edge of Pulse Per Second (PPS) emitted by Global Position System (GPS), and the data is sent to the public port of mobile telephone network by General Packet Radio Service (GPRS). The remote upper computer gets the data and calculates corona loss of each bundle conductor by visiting public port. The experiment results show that the system can monitor the corona loss accurately. The whole study is helpful in the further research of UHV transmission line’s corona in different environment and weather.

PL

Zaprezentowano metodę monitorowania strat powodowanych ulotem (wyładowaniem koronowym) w liniach transmisyjnych wysokiego napięcia. Do tego celu wykorzystano metalizowaną membranę. Układ składa się z trzech modułów: komputera, systemu akwizycji napia) ęcia i systemu akwizycji prądu ulotu. Linia transmisyjna jest pokryta na pewnej długości cienką metalizowaną membraną. Do lokalizacji ulotu zastosowano moduł GPS a do transmisji danych moduł GPRS.

4

Lycium barbarum polysaccharide protects HSF cells against ultraviolet-induced damage through the activation of Nrf2

84%

Liang B. , Peng L. , Li R. , Li H. , Mo Z. , Dai X. , Jiang N. , Liu Q. , Li Z. , Zhu H.

|

tom 23

EN

Background: Lycium barbarum polysaccharide (LBP) is considered an antioxidant agent. NF-E2-related factor-2 (Nrf2) is an important regulator for protection against UV damage. In this study, we verified the performance of LBP and the correlation between LBP and Nrf2. Methods: HSF cells were treated with LBP to determine dose and time dependencies. An antioxidant response element (ARE) reporter was designed to monitor the activity of the Nrf2 antioxidant pathway. Results: For HSF cells, the optimal LBP treatment was 300 μg/ml for 3 h. The AREreporter assay showed that LBP could increase the robustness of p-Nrf2. Treatments with genistein and LY294002 reduced of nuclear p-Nrf2 after 24 h. LBP increased the level of nuclear Nrf2, which functions by both phosphorylation and nuclear translocation. Silencing Nrf2 led to increased reactive oxygen species (ROS) levels, lower cell viability, and decreased superoxide dismutase (SOD) and glutathione peroxidase (GSP-PX) levels. This induced a higher level of lipid peroxide (LPO). However, LBP could decrease the levels of ROS and LPO and enhance the levels of SOD and GSP-PX. Conclusion: LBP protects HSF cells against UV damage via the regulation of Nrf2.

5

Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz)

67%

Li R. , Ji Y. , Fan J. , Yang C. , Yao Y. , Zhou Y. , Duan R. , Liu J. , Fu S. , Hu X. , Guo J.

|

nr 11

EN

Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4°C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava’s high sensitivity to low temperature.