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1
Content available Optimizing manufacturing conditions of polymer microspheres as cell carriers for modular tissue engineering
100%
Mielan B. , Pamuła E.
Engineering of Biomaterials
|
2020
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tom Vol. 23, no. 156
2--9
EN
Microspheres (MS) made of biostable polymer, namely polystyrene, have been used as substrates for cell culture enabling rapid cell expansion in dynamic conditions. However, due to non-resorbability, polystyrene (PS) MS when repopulated with cells cannot be directly used in tissue engineering. Our concept was to produce MS from resorbable polymer – poly(L-lactide- -co-glycolide) (PLGA) as a support for adherent cells, e.g. osteoblasts. We hypothesize that such MS can be applied to the injured site to act as cell carriers or as modules for modular tissue engineering (MTE). In this article, we present the results of optimizing the PLGA MS manufacturing conditions via oil-in-water emulsification. Due to such a technique, MS with the required size, size distribution and properties suitable for cell culturing can be obtained. Three parameters of the oil-in-water emulsification were examined: the stirring speed of a water phase during MS manufacturing, the surfactant concentration, i.e. poly(vinyl alcohol) (PVA) in a water phase and concentration of PLGA in dichloromethane (DCM) as an oil phase. The results proved that the 7.5% PLGA concentration in DCM solution as an oil phase, the 0.5-2% concentration of PVA solution as a water phase and the stirring speed of water phase of 1000 rpm provided MS with the 160 μm mean diameter, which is suitable for cell culture. Moreover, the developed sieving and cleaning procedures were efficient to collect MS with the mean diameter of 280 μm, the more coherent size distribution and the ability to sink in the cell culture medium. The presence on the bottom of cell culture wells is crucial for MTE.
2
Content available Hydrogel scaffolds for medical applications obtained by indirect 3D-printing method
100%
Pietryga K. , Pamuła E.
Engineering of Biomaterials
|
2019
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tom Vol. 22, no. 153 spec. iss.
93
3
Content available Poly(L-lactide-co-glycolide) microparticles emulsified by mixing and in a microfluidic device for potential bottom-up bone tissue engineering
80%
Marecik S. , Krok-Borkowicz M. , Pamuła E.
Engineering of Biomaterials
|
2023
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tom vol. 26, no. 169
18--22
EN
The aim of this study was to obtain degradable poly(Llactide-co-glycolide) (PLGA) microparticles (MPs) with a controlled size for bottom-up bone tissue engineering. The particles were produced using the classical single water/oil emulsification method by mixing with a magnetic stirrer and by using a novel approach based on the application of a microfluidic device. This study involved a thorough investigation of different concentrations of PLGA and poly(vinyl alcohol) (PVA) during microparticle fabrication. The oil phase was PLGA dissolved in dichloromethane or ethyl acetate at 1%, 2% and 4% w/v concentrations. The water phase was an aqueous solution of PVA at concentrations of 0.5%, 1%, 2%, 2.5%, 4% and 5% w/v. The size and size distribution of the MPs were evaluated with an optical microscope. Obtained MPs were incubated in contact with osteoblast-like MG-63 cells and after days 1 and 3, the cell viability was evaluated using the reduction of resazurin and the fluorescence live/dead staining. The results showed that for each concentration of PVA, the size of the MPs increased with an increase in the concentration of PLGA in the oil phase. The MPs obtained with the use of the microfluidic device were characterized by a smaller size and lower polydispersity compared to those obtained with emulsification by mixing. Both methods resulted in the generation of MPs cytocompatible with MG-63 cells, what paves the way to consider them as scaffolds for bottom-up tissue engineering.
4
Content available Optimization of the calcium carbonate particles manufacturing process by the precipitation method and biological evaluation with MG-63 cells
80%
Pudełko-Prażuch I. , Wojtanek K. , Pamuła E.
Engineering of Biomaterials
|
2023
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tom vol. 26, no. 170
20--27
EN
As a natural mineral, calcium carbonate (CaCO3) is widely investigated for various medical applications. It is a biocompatible material characterized by high degradation rate and great osteoconductivity. Many researchers evaluate CaCO3 in the form of particles as a candidate for use in drug delivery systems. In this study we present an optimization of the process of producing CaCO3 particles by the precipitation method with the use of combinations of different time of ultrasound treatment and surfactant concentrations used. Depending on the synthesis conditions, various sizes of particles were fabricated. The particles were loaded with sodium alendronate (Aln, 5% or 10% by weight) with a relatively high encapsulation efficiency between 40 and 50%, depending on the amount of Aln added and the drug loading of approximately 9% for both cases. MG-63 osteoblast-like cells were contacted with 10% wt./vol extracts of fabricated particles to assess their cytotoxicity. None of the extracts investigated was found to be cytotoxic. Furthermore, an in vitro study in direct contact of MG-63 cells with particles suspended in culture medium was performed. The results showed that the fabricated particles are cytocompatible with osteoblast-like MG-63 cells. However, the higher the concentration of the particle suspension and the greater the amount of alendronate present in the solution, the lower the metabolic activity of the cells was measured. The presented method of CaCO3 particles manufacturing is simple, cost-effective, and allows one to fabricate particles of the required size and shape that are cytocompatible with MG-63 cells in defined concentrations of particle suspensions.
5
Content available Bioactive ceramic coating formed on Ti bone wedge
80%
Kazek-Kęsik A. , Krok-Borkowicz M. , Pamuła E. , Simka W.
Engineering of Biomaterials
|
2020
|
tom Vol. 23, no. 158 spec. iss.
50
6
Content available Manufacturing and characterization of gellan gum – zinc oxide composites as potential biomaterials for wound treatment
80%
Macyk A. , Kusibab A. , Pamuła E.
Engineering of Biomaterials
|
2023
|
tom vol. 26, no. 168
2--8
EN
This study aimed to produce gellan gum-based hydrogels with the addition of zinc oxide as a potential dressing material. Hydrogels with ZnO concentrations of 0.01%, 0.02% and 0.04% were prepared, micrometric and nanometric ZnO particles were used, and a CaCl2 crosslinker was added to one part of the samples. All samples (14 types) produced by the freeze drying method were characterized with high swelling properties (>2000%), what is important to ensure the absorption of exudates from wounds. Samples with ZnO particles cross-linked with CaCl2 lost less mass after incubation in aqueous media and were characterized by better dimensional stability than those without crosslinking. The pH of the extracts of the samples containing ZnO particles was more neutral (pH 7.0-7.6) than that of the control gellan gum samples (pH of 5.5-6.1). The zinc release from cross-linked samples was twice as high for those containing nanometric particles than for micrometric particles (1.94 ± 0.04 mg/l and 0.93 ± 0.02, respectively). Relatively large amounts of released zinc species in the case of samples containing ZnO nanoparticles are promising in the context of the antibacterial properties and treatment of infected wounds. A lower amount of zinc released from samples with ZnO microparticles could be sufficient to prevent the development of the infection. Furthermore, both materials show satisfactory cytocompatibility with L929 fibroblasts, as shown by Alamar blue and live/dead viability tests, making them prospective candidates for wound healing
7
Content available Mesenchymal stem cells proliferation and osteogenic differentiation on polymeric scaffolds and microspheres for bone tissue engineering
80%
Walczak K. , Krok-Borkowicz M. , Pamuła E.
Engineering of Biomaterials
|
2023
|
tom vol. 26, no. 170
14--19
EN
In this study, we aimed to compare how the microstructure and architecture of polymer supports influence adhesion, growth and differentiation of human mesenchymal stem cells (hMSC) in the context of bone tissue engineering. We manufactured poly(L-lactide-co-glycolide) (PLGA) three-dimensional supports in the form of microspheres by emulsification and porous scaffolds by solvent casting/ porogen leaching. HMSC were seeded on both materials and on control tissue culture polystyrene (TCPS, bottom of the wells) and cultured in basal or osteogenic medium for 1, 3, 7 and 14 days. HMSC proliferation and osteogenic differentiation were studied using lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) assays, respectively. Furthermore, cell morphology and viability were analyzed after live/dead fluorescence staining. The results show that the optimized emulsification conditions allowed the production of PLGA microspheres with a median size of 95 µm. The PLGA scaffolds had a porosity of 82.1% ± 4.2% and a pore size of 360 µm ± 74 µm. HMSC cultured on control TCPS in osteogenic medium were more spread and polygonal than those in basal medium. They were characterized with a lower proliferation rate, as shown by the LDH results, but higher ALP activity. This suggests that hMSC osteogenic differentiation was achieved. The same tendency was observed for cells cultured on microspheres and scaffolds. Cell proliferation was more efficient on both materials and control in growth medium as compared to differentiation medium. The amount of ALP, i.e. a marker of osteogenic differentiation, was elevated, as expected, in differentiation medium. However, on day 14 cells cultured on the scaffolds in basal medium exhibited the same osteogenic potential as those cultured in differentiation medium. In general, both microspheres and scaffolds promoted hMSC adhesion, proliferation, and osteogenic differentiation and may be used for bone tissue engineering.
8
Content available Poly(sebacic anhydride) microparticles loaded with curcumin for pulmonary purposes
71%
Kwiecień K. , Reczyńska-Kolman K. , Niewolik D. , Jaszcz K. , Pamuła E.
|
|
tom Vol. 24, no. 162
7--12
EN
Microparticles (MPs) made of fast biodegrading biomaterials, loaded with drugs, are considered a superior treatment method for pulmonary infections. One of the promising biomaterials for obtaining such a drug delivery system (DDS) is poly(sebacic anhydride) (PSA) due to its favourable degradation kinetics and mechanism. In this paper, we present a study of manufacturing MPs from PSA loaded with curcumin (CU) for pulmonary purposes. MPs were manufactured by oil-in-water emulsification; their morphology and size distribution were evaluated using optical microscopy, while the encapsulation efficiency and drug loading were obtained by the fluorometric assay. The cytotoxicity of the MPs, both the empty ones and loaded with CU, was analysed by in vitro tests with BEAS-2B human lung epithelial cells. To this end, metabolic activity by AlamarBlue assay and fluorescent staining (DAPI/ eosin) of the cells were performed. The MPs produced were round, regular in shape with diameters in the range of 1-5 µm and of yellow colour originating from CU. The CU encapsulation efficiency ranged from 42% to 55% and decreased with a higher CU ratio. The drug loading ranged from 4% to 11% and increased at a higher CU ratio. Both empty and CU-loaded MPs did not show a cytotoxic effect at concentrations up to 10 µg/ml.
9
Content available Degradation studies of poly(diol citrates) for vascular tissue engineering purposes
71%
Kwiecień K. , Flis A. , Koper F. , Kasprzyk W. , Pamuła E.
Engineering of Biomaterials
|
2020
|
tom Vol. 23, no. 158 spec. iss.
65
10
Content available Development of polyester coatings on poly(ethylene terephtalate) prostheses for vascular tissue engineering
71%
Flis A. , Calik L. , Koper F. , Kasprzyk W. , Pamuła E.
Engineering of Biomaterials
|
2021
|
tom Vol. 24, no. 163
82
11
Content available The encapsulation of antibacterial drugs in polymer nanoparticles and their use in drug delivery systems on ZrO2 scaffold with bioactive coating
71%
Pudełko I. , Desante G. , Pamuła E. , Schickle K. , Krok-Borkowicz M.
Engineering of Biomaterials
|
2021
|
tom Vol. 24, no. 161
21--27
EN
Bone infections are a challenging problem as they may cause a permanent patient disability and even death. Additionally, their relapse rate is relatively high. The implantation of a local drug delivery system can be an effective way to fight bone infections. In this study, we present the process of surface bioactivation and immobilization of nanoparticles loaded with drugs. Our aim was to improve osseointegration of the ZrO2 surface by coating it with a bioactive layer containing poly(L-lactide-co-glycolide)(PLGA) nanoparticles (NPs) loaded with antibacterial drugs (gentamicin and bacitracin) using a biomimetic precipitation method. The ZrO2 substrates were prepared via pressing and sintering. The CaP-coating was obtained by immersing the substrates in ten-times concentrated simulated body fluid (10×SBF). NPs were prepared by the double emulsion method and the drug loading in NPs was assessed. Thus obtained NPs were applied on bioactivated ceramic substrates by the drop-casting method or by introducing them in the 10×SBF solution during the bioactivation process. The NPs were visualized using scanning electron microscopy (SEM). The NPs size and the Zeta potential were measured using dynamic light scattering (DLS) method. The microstructure of the coating and the efficiency of the NPs incorporation were tested by SEM. In this study, we proved the presented process to be an effective way to obtain biomaterials that could be used as drug delivery systems to treat bone infections in the future.
12
Content available Lipid microparticles as quercetin carriers for the treatment of burn wounds: manufacturing, properties, and in vitro studies on macrophages polarization
71%
Stępień J. , Kusibab A. , Sousa A. B. , Barbosa J. N. , Pamuła E.
Engineering of Biomaterials
|
2023
|
tom vol. 26, no. 171
2--11
EN
Burn wounds are a unique type of injury that can affect the entire body and cause irreversible damage. They are characterized by significant morbidity and mortality due to the pathophysiology of the healing process manifested by unremitting inflammation, leading to a critical need to search for new treatments. This study focuses on the development of drug delivery systems in the form of lipid microparticles loaded with quercetin, as an agent to combat acute inflammation in burn wounds. We aimed to explore the effect of quercetin in modulating macrophage polarization from proinflammatory (M1) to anti-inflammatory (M2) phenotype. The absence of a cytotoxic effect of the produced particles on macrophages, as well as the lack of negative effects on their morphology was proven. The study confirmed the ability of quercetin and quercetin-loaded lipid microparticles to modulate macrophage polarization in an anti-inflammatory direction, based on the analysis of their surface markers expression performed with the use of flow cytometry. With the use of quercetin, the expression of M2 specific marker increased. Furthermore, better results were obtained for encapsulated quercetin, confirming the necessity of encapsulation to increase the therapeutic potential.
13
Content available Synthesis and characterization of novel amphiphilic poly(2- oxazoline)-based copolymers for surface modification of biomaterials
61%
Tryba A. M. , Kroneková Z. , Mičušík M. , Paluszkiewicz C. , Pamuła E. , Kronek J.
Engineering of Biomaterials
|
2019
|
tom Vol. 22, no. 153 spec. iss.
87
14
Content available Cytocompatibility tests of Poly(diol citrate) polymers in contact with adipose tissue derived stem cells for vascular tissue engineering purposes
61%
Flis A. , Travnickova M. , Koper F. , Bacakova L. , Kasprzyk W. , Pamuła E.
Engineering of Biomaterials
|
2019
|
tom Vol. 22, no. 153 spec. iss.
76
15
Content available Modification of titanium implant surface for animals
61%
Kazek-Kęsik A. , Sadkowska A. , Reczyńska-Kolman K. , Kalemba-Rec I. , Śmiga-Matuszowicz M. , Pamuła E. , Simka W.
Engineering of Biomaterials
|
2021
|
tom Vol. 24, no. 163
16
16
Content available Spectroscopy analyses of structure-property correlations in citrate-based elastomeric biomaterials
51%
Koper F. , Świergosz T. , Żaba A. , Flis A. , Trávníčková M. , Bačáková L. , Pamuła E. , Bogdał D. , Kasprzyk W.
Engineering of Biomaterials
|
2021
|
tom Vol. 24, no. 163
94
17
Content available Inhalable drug delivery system based on poly(sebacic acid) and azithromycin for the treatment of bacterial infections
51%
Reczyńska-Kolman K. , Kwiecień K. , Knap K. , Niewolnik D. , Kazek-Kęsik A. , Płonka J. , Jaszcz K. , Pamuła E.
Engineering of Biomaterials
|
2021
|
tom Vol. 24, no. 163
86
18
Content available Phenolic plant extract enrichment of enzymatically mineralized hydrogels
36%
Douglas T. E. L. , Lopez-Heredia M. A. , Pułczyńska A. , Łapa A. , Pietryga K. , Schaubroeck D. , Santos S. A. O. , Pais A. , Brackman G. , Schamphelaere K. d. , Samal S. K. , Keppler J. K. , Bauer J. L. , Chai F. , Blanchemain N. , Coenye T. , Pamuła E. , Skirtach A. G.
Engineering of Biomaterials
|
2019
|
tom Vol. 22, no. 149
2--9
EN
Hydrogel mineralization with calcium phosphate (CaP) and antibacterial activity are desirable for applications in bone regeneration. Mineralization with CaP can be induced using the enzyme alkaline phosphatase (ALP), responsible for CaP formation in bone tissue. Incorporation of polyphenols, plant-derived bactericidal molecules, was hypothesized to provide antibacterial activity and enhance ALP-induced mineralization. Three phenolic rich plant extracts from: (i) green tea, rich in epigallocatechin gallate (EGCG) (herafter referred to as EGCG-rich extract); (ii) pine bark and (iii) rosemary were added to gellan gum (GG) hydrogels and subsequently mineralized using ALP. The phenolic composition of the three extracts used were analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MSn). EGCG-rich extract showed the highest content of phenolic compounds and promoted the highest CaP formation as corroborated by dry mass percentage meassurements and ICP-OES de-termination of mass of elemental Ca and P. All three extracts alone exhibited antibacterial activity in the following order EGCG-rich > PI > RO, respectively. However, extract-loaded and mineralized GG hydro-gels did not exhibit appreciable antibacterial activity by diffusion test. In conclusion, only the EGCG-rich extract promotes ALP-mediated mineralization.
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