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1

Occurrence of antibodies against Chlamydophila abortus in sheep and goats in the Slovak Republic

100%

Cislakova L. , Halanova M. , Kovacova D. , Stefancikova A.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 2

EN

Chlamydophila abortus is one of the most important causative agents of enzootic abortion and other chlamydial infections of sheep and goats. The presence of specifi c serum antibodies to Chlamydophila abortus was studied in sheep and goats breeding in the Slovak Republic by the complement fi xation test. 22,040 sheep and goats were examined during 5 years. Specifi c anti-Ch. abortus antibodies were found in 2,360 out of 20,878 sheep sera examined (11.7%), and in 85 out of 1,162 examined goats (7.7%). The occurrence of antichlamydial antibodies indicates the importance of performing screening examinations in commercial breeding with the aim of reducing the spread of this disease between animals, and also interrupting the spread and transmission from animals to human.

2

Badania serologiczne w kierunku boreliozy i erlichiozy swin i krow na Lubelszczyznie

76%

Winiarczyk S. , Adaszek L. , Stefancikova A. , Pet'ko B. , Cislakova L. , Puchalski A.

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tom 63

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nr 05

561-565

EN

The aim of this paper was the detection of the specific antibodies anti-Borrelia and anti-Ehrlichia in the sera of 28 sick pigs and 29 sick cows from the area of the Lublin voivodeship. ELISA tests, and Western blot were conducted. In ELISA tests, 17.86% sera of swine were positive for spirochetes and 7.14% for Ehrlichia. In a bovine group 44.83% of sera specimens were positive for Borrelia, and 20.69% for Ehrlicha. All sera that were positive in ELISA tests for spirochetes were examined additionally in Western blot method for the presence of antibodies against Borrelia afzelii (13 bovine sera, and 5 swine sera). In 8 out of 13 bovine sera (61.54%) and in one out of 5 (20%) swine sera that were positive in the ELISA test, specific antibodies against Borrelia were detected. In all cases IgG immunoglobins reacted strongly with the antigens: 31, 39, 41, 66 kDa of B. afzelii. The results revealed a low specificity of the ELISA test for the detection of borreliosis and confirmed an endemic occurrence of Borrelia and Ehrlicha in the area of the Lublin voivodeship

3

Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

76%

Stefancikova A. , Derdakova M. , Stepanova G. , Pet'ko B. , Szestakova E. , Skardova I. , Cislakova L.

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nr 2

211-216

EN

Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B31 and Ir112, B 31 and K 24 differed statistically significantly ( test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K 24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir 105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04% The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close strains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.

4

Anti-Borrelia antibodies in rodents: important hosts in ecology of Lyme disease

63%

Stefancikova A. , Bhide M. , Pet'ko B. , Stanko M. , Mosansky L. , Fricova J. , Derdakova M. , Travnicek M.

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tom 11

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nr 2

EN

Data presented in this study focuses on the presence of anti-Borrelia antibodies in small mammals from Eastern Slovakia during 2000-2003. The total seropositivity observed was 18.78% in rodents. Amongst all species, the total seroprevalence in Apodemus flavicolis was the highest (20.87%), followed by Apodemus agrarius (19.58%) and Clethrionomys glareolus (11.11%). However, the prevalence in Apodemus flavicolis during the year 2000-2001 was higher (26.72%), which reduced to 10.60% in 2002-2003. To compare the year range of seroprevalence in other small mammals was not feasible due to the small sample number. Area-wise distribution of anti-Borrelia antibodies was even (18.75% to 20%) in this study, except in the Boťany province (0%). This confirms the equal distribution of Borrelia spirochetes in the other 3 localities. Prevalence of anti-Borrelia antibodies during summer was significantly higher than during autumn and early spring. The overall study also reviews the importance of small mammals in Lyme disease ecology.

5

Seroprevalence of anti-Borrelia burgdorferi antibodies in sheep and goats from mountainous areas of Slovakia

63%

Travnicek M. , Stefancikova A. , Nadzamova D. , Stanko M. , Cislakova L. , Pet'ko B. , Mardzinova S. , Bhide M. R.

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tom 09

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nr 2

6

Epidemiology of Lyme borreliosis in Slovakia

51%

Pet'ko B. , Tresova G. , Stefancikova A. , Peterkova J. , Prokopcakova H. , Cislakova L. , Stanko M. , Fricova J. , Skardova I. , Sesztakova E. , Nadzamova D.

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tom 44

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nr 3