Wyniki wyszukiwania - Biblioteka Nauki

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Ocena stanu wiedzy młodych ludzi na temat HIV/AIDS

100%

Janiszewska E. , Pluta D. , Dobosz T.

Alergia Astma Immunologia - przegląd kliniczny

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2019

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tom 24

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nr 1

24-29

EN

Introduction. Acquired Immunodeficiency Syndrome (AIDS) caused by human immunodeficiency virus (HIV) is one of the most serious infectious diseases on a global scale. Since the outbreak of the epidemic there was 78 million people infected with HIV, 35 million of whom have died. The AIDS control in Poland began in 1988. Aim. Assessment the knowledge level of young Polish people about AIDS. Materials and methods. The survey was conducted among 346 people utilizing the social media channels. They received 11 questions including the basic definitions, ways of infection and diagnosis of AIDS. Results. The results of our study confirm that people have insufficient knowledge of this serious disease. Less than 40% of all respondents could point the most common paths of infection and the time of first symptoms after exposure. Nearly 50% knew that AIDS and HIV are not equal terms. Conclusions. There is a need to raise awareness about HIV and AIDS. Young people should learn much more about mechanisms, symptoms and particularly prevention of this still untreatable disorder.

PL

Wprowadzenie. Zespół nabytego niedoboru odporności, AIDS (ang. Acquired Immunodeficiency Syndrome), wywoływany przez ludzkiego wirusa niedoboru odporności (HIV, ang. human immunodeficiency virus) to jedna z najpoważniejszych chorób w skali świata. Szacuje się, że od wybuchu epidemii zakażeniu wirusem uległo 78 milionów ludzi, a około 35 milionów chorych zmarło. W Polsce epidemiologia i problematyka AIDS oraz HIV została podjęta w 1988 roku. Cel pracy. Ocena stanu wiedzy młodych ludzi na temat HIV i AIDS. Materiały i metody. Przeprowadzono ankietę internetową wśród 346 osób. Respondenci otrzymali 12 pytań na temat podstawowych pojęć, dróg zakażenia oraz diagnostyki AIDS. Wyniki. Wyniki ankiety potwierdzają, że wiedza młodych ludzi na temat HIV i AIDS jest niewystarczająca. Niecałe 40% ankietowanych potrafiło wskazać poprawnie główne drogi infekcji oraz prawidłowy przedział czasu pomiędzy ekspozycją a pojawieniem się pierwszych niespecyficznych objawów. Blisko połowa badanych miała świadomość, że pojęcia AIDS i HIV nie są tożsame. Wnioski. Istnieje potrzeba szerzenia wiedzy na temat HIV i AIDS, szczególnie wśród młodych ludzi. Tylko świadomość mechanizmu choroby, dróg zakażenia, a przede wszystkim możliwej profilaktyki, pozwoli na zminimalizowanie ryzyka zachorowania na chorobę, na którą obecnie wciąż nie wynaleziono remedium.

2

Plany ścieżki muzealnej Uniwersytetu Medycznego we Wrocławiu

100%

Pluta D. , Król M. , Dobosz T.

Opuscula Musealia

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2019

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tom 26

183-190

EN

People have always been interested in discovering their past and seeking answers to their existential questions. Therefore institutions like museums, which collect exhibits related to the history and development of humans, have been known since antiquity. However, museology today has changed and is no longer confined to establishments related to popular fields like painting, sculpture and archaeology. Nowadays, there are also museums dedicated to medical sciences, which present the history of humanity in the sphere of its biological development. The Medical University in Wrocław can boast of several such establishments, although so far they have been treated as separate institutions. For this reason, the idea was created to combine them all into a single museum route, and to make it available to a broader visitor base. Thanks to this, people can view items from old and modern medicine practice located in many different sites, allowing them to deepen their knowledge and expand their interests. To make it easier for visitors to navigate through such a diverse complex, a special museum path has been created, along with a description of each location.

3

Mokre preparaty muzealne – konserwacja i receptury

100%

Pluta D. , Siuta J. , Czerbak M. J. , Dobosz T.

Opuscula Musealia

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2019

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tom 26

191-198

EN

Due to the uniqueness of wet museum exhibits, there is often a shortage of adequately trained people to carry out conservation work in museum units. Unfortunately, in many cases, the museum exhibits require immediate work. There is usually a visible loss of preservative fluid, or no fluid at all if it has evaporated. Moreover, chipped lids and damaged jars frequently occur. Some exhibit labels are damaged or torn off. Some items have been exhibited incorrectly, but when they are transferred to a new vessel or the fluid is replaced, they gain added value. Although there is a great need for conservation work, many museologists fail to carry it out. A significant problem is the absence of unified conservation procedures or guidelines which could be applied for these types of cases. This paper includes conservation formulas and recipes used at the Molecular Techniques Unit. The authors of this paper hope that it will be helpful to all those who deal with preserving wet museum exhibits.

4

Comparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores

88%

Nawrot U. , Wlodarczyk K. , Wrobel M. , Wasik A. , Dobosz T.

Acta Biochimica Polonica

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2010

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tom 57

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nr 4

567-571

EN

The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using Aspergillus-specific primers and SYBR Green I dye or TaqMan probes targeting 28S rRNA gene. All methods allowed the detection of Aspergillus at the lowest tested density of water suspensions of spores (101 cells/ml). The highest DNA yield was obtained using the ZR Fungal/Bacterial DNA kit, YeastStar Genomic DNA kit, and QIAamp DNA Mini kit with mechanical cell disruption. The ZR Fungal/Bacterial DNA and YeastStar kits showed the highest sensitivity in examination of blood samples spiked with Aspergillus (100 % for the detection of 102 spores and 75 % for 101 spores). Recently, the enzymatic method ceased to be recommended for examination of blood samples for Aspergillus, thus ZR Fungal/Bacterial DNA kit and QIAamp DNA Mini kit with mechanical cell disruption could be used for extraction of Aspergillus DNA from clinical samples.

5

Analysis of museum labels description

75%

Karpiewska A. , Iwan D. , Szymroszczyk P. , Lenard E. , Halama M. , Pluta D. , Dobosz T.

Opuscula Musealia

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2019

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tom 26

143-156

EN

The aim of the study was to analyse the content of museum labels from various periods in terms of their usefulness in creating new labels for exhibits included in museum collections. Nearly 5,000 museum labels from the years 1811 to 2017 were reviewed, for exhibits at the Museum of Zoology of the Polish Academy of Sciences in Warsaw, Herbarium of the University of Wroclaw, and the Museum of Forensic Medicine at the Medical University of Wroclaw. On the basis of the collected information, an attempt was made to develop a ‘universal label’template, including a range of necessary information from the point of view of managing and maintaining the accessibility of the relevant collection.

6

VDR gene single nucleotide polymorphisms and their association with risk of oral cavity carcinoma

51%

Małodobra-Mazur M. , Paduch A. , Lebioda A. , Konopacka M. , Rogoliński J. , Szymczyk C. , Wierzgoń J. , Maciejewski A. , Chmielik E. , Jonkisz A. , Półtorak S. , Dobosz T.

Acta Biochimica Polonica

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2012

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tom 59

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nr 4

627-630

EN

Vitamin D3 (1,25(OH)2D3 (1,25-dihydroxyvitamin D3)) is a hormone playing a crucial role in numerous biological processes in the human body, including induction and control of cell proliferation and differentiation. Numerous data relate the vitamin D3 level with various types of cancer. It has been suggested that SNPs in the vitamin D3 receptor (VDR) gene might influence both the risk of cancer occurrence and cancer progression. The aim of this study was to search for genetic correlations between individual SNPs in the VDR gene and the risk of oral cavity carcinoma. Two SNPs were selected based on the literature and our previous results. Seventy-three patients with squamous cell carcinoma of the head and neck and one hundred control subjects were investigated. Two SNPs in the VDR gene were genotyped in minisequencing reactions followed by capillary electrophoresis. Hardy-Weinberg equilibrium (HWE), the χ2 test and logistic regression were used for statistical analysis. The SNP rs2238135 in the VDR gene displayed statistical differences in frequency between the tested groups (p=0,0007). Furthermore, the G/C genotype of the rs2238135 in the VDR gene was characterized by a 3.16 fold increased risk of oral cavity carcinoma. The obtained results provide evidence for a genetic association between rs2238135 in the VDR gene and the occurrence and risk of oral cavity cancer.