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tom Nr 4
44--46
PL
Od wielu lat obserwujemy popularyzację rozwiązań związanych z komputerową analizą obrazu (ang. computer vision, CV) w rozmaitych gałęziach techniki. Smartfony i aparaty fotograficzne rozpoznają twarze na zdjęciach, a rozmaite koncerny pracują nad samochodami, które prowadzi się bez udziału człowieka. Tymczasem od wielu lat dostępne są coraz doskonalsze systemy wizyjne dla przemysłu, które pozwalają na optymalizację zadań na liniach produkcyjnych.
PL
W pracy przedstawiono praktyczną realizację demonstratora radaru FMCW (ang. Frequency-Modulated Continuous-Wave) z wykorzystaniem urządzenia NI USRP (ang. Universal Software Radio Peripheral) X310. Szczegółowo opisana implementacja opiera się na architekturze SDR (ang. Software-Defined Radio). Podczas serii eksperymentów dowiedziono możliwości wykrycia obiektów w postaci samochodów poruszających się po drogach przy użyciu opracowanego radaru zbudowanego z urządzenia USRP bez dodatkowych zewnętrznych analogowych układów przemiany częstotliwości. W pracy przestawiono zarówno implementację wstępnego przetwarzania sygnałów w czasie rzeczywistym w układzie FPGA jak i obliczenia wykonywane w oparciu o uprzednio zarejestrowane surowe dane radarowe. Na podstawie wyników pomiarów zidentyfikowano ograniczenia prezentowanego rozwiązania oraz przedyskutowano przydatność urządzenia USRP w prototypowaniu radarów FMCW.
EN
The paper describes practical implementation of FMCW (Frequency-Modulated Continuous-Wave) radar demonstrator using NI USRP (Universal Software Radio Peripheral) device. Described in detail implementation is based on SDR (Software-Defined Radio) architecture. During a series of experiments the possibility of detection of objects such as moving cars using implemented USRP-based radar with no external frequency-conversion devices has been proven. The paper presents detailed description of both real-time processing in FPGA device and offline signal processing of recorded data in the MATLAB environment. Basing on measurements results the limitations of the presented solution have been identified and the applicability of USRP device in prototyping of FMCW radars has been discussed.
EN
The aim of the research was to compare selected biochemical parameters of rabbits’ blood (crossbreeds) depending on the kind of diet. Twelve rabbits at the age of 2 months and at a body weight of 1.5 kg were chosen for this experiment. The animals were divided into two research groups depending on the kind of diet they were fed. The first group consisted of rabbits fed on an all-mash diet while the second group was fed according to a typical ecological model. After 7 months of the experiment blood was collected for analysis from each animal. In the blood serum samples, the activities of the following enzymes were determined: aspartate aminotransferase (AST), alanine aminotransferase (ALT), as were the concentration of glucose, urea, total protein, protein fractions and the lipid proflile (total cholesterol, HDL and LDL fractions) as well as the concentration of triglycerides. This research showed statistically significant diet-dependent differences among all biochemical parameters of blood apart from HDL fractions.
EN
The aim of this work was to evaluate the fertilizing capacity of bulls on the basis of the assessment of fresh and thawed semen, following the swim-up procedure and immunological assay with IgA and IgG antisperm antibodies. In addition, the suitability of immunological reaction with IgG and IgA antisperm antibodies for seminological assessment of bull semen was examined. Tests were conducted with semen originating from Holstein-Friesian bulls. Ejaculates were collected twice from 15 two-year-old males, yielding 30 samples of semen. After the calculation of sperm concentration and motility, the samples were diluted with Biociphos-Plus® and frozen in straws. After thawing, sperm concentration and motility were calculated again (an average of 101.4 million sperm/ml with a motility of around 21%), and the spermatozoa were subjected to swim-up. At the same time, frozen-thawed bull semen was tested for the presence of antisperm antibodies, using SpermMar Tests for IgG and IgA. According to the records of the insemination centre, 53 515 insemination treatments had been conducted with semen of selected bulls. In a population of 30 324 cows, the calculated insemination index amounted to 1.76. Half of the cows which were qualified for insemination had already been fertilized with the first treatment (ca 52.8%). After the swim-up, there was an average of 4.5 million hyperactivated spermatozoa with a motility of around 41%. Furthermore, spermatozoa associated with IgG antisperm antibodies were found in 20 ejaculates, i.e. in around 66.67% of all semen samples obtained. The spermatozoa reacted positively with IgA antibodies in as many as 23 samples, which constituted 76.67% of all ejaculates obtained. Correlations between the routinely analyzed semen parameters and the selected parameters (immunological, swim-up) which were demonstrated in this work, did not appear to be sufficiently convincing. Therefore, it seems necessary to repeat this study with a population of clinically healthy, fertile bulls, as well as with juvenile bulls newly introduced to the insemination centre or with bulls with reduced fertility.
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nr 12
s.839-842,tab.,bibliogr.
EN
The aim of the study was to compare the values of selected parameters of post-slaughter carcass evaluation and rabbit meat quality indices (protein, fat and water content) depending on the type of diet used. The experiment involved 12 rabbit - crossbreeds at the age of 2 months and at a body weight of 1.5 kg. The animals were divided into two groups with regard to the type of diet administered. Group I consisted of rabbits fed on an all-mash diet, whereas group II was fed according to the typical ecological model. After 7 months of the experiment all the animals were slaughtered and afterwards the slaughter analysis was performed taking into consideration the following: pre-slaughter body weight, hot carcass weight, weight of the stomach (with or without digesta). After the cooling of the carcasses, muscle samples of the saddle and the thigh were taken for subsequent analyses. Meat quality was estimated on the basis of the determination of protein, fat and water content. It was found that the diet significantly influenced fat and water content in rabbit meat. The muscle samples taken from the ecologically fed rabbits (group II) had a higher water level (saddle - 73.58%; thigh - 74.87%) and a lower fat content (saddle - 1.31%; thigh - 1.51%) compared to the samples taken from the rabbits fed on granulated feed. No significant differences in protein content between the two experimental groups were found. The slaughter yield in both groups did not exceed 50%. The higher but statistically unconfirmed slaughter yield was obtained from rabbits fed ecologically.
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tom 64
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nr 11
1340-1343
EN
The aim of the studies was to assess the protein composition of the polar fox’s (Alopex lagopus L.) semen plasma with the use of the electrophoretic method (SDS-PAGE) and to find out whether there are statistically important differences in the protein profiles between the beginning and the end of the reproductive period and between the selected proteins and the semen traits. The examined material was the semen plasma obtained with the use of the manual method from nine nine-year-old polar foxes. Both in the plasma obtained at the beginning and at the end of the season ten protein fractions (10.9÷107.5 kDa) were distinguished. On the basis of the densitometric analysis of electrophoretic images, a quantitative decrease of multimolecular forms at the end of the reproductive period was observed. This may indicate either a decreasing synthesis of proteins in the reproductive system or partial proteolysis in progress. The analysis of correlations, in turn, indicated significant relationships (p < 0.05) between the masses of protein fractions (107.5 and 58.9 kDa) and the volume of the semen collected at the beginning of the season (r = -0.76 and r = -0.72 respectively): between proteins 74.8 and 34.9 kDa and the volume of the semen at the end of the season (r = 0.92 and r = 0.79 respectively), and also between proteins 58.9; 34.9; 27.0 and the percentage of the sperm with major flaws in the semen collected at the beginning of the reproductive season (r = 0.69; r = 0.89 and r= -0.76 respectively). Even though significant correlations between the plasma proteins and the traits of the semen obtained from young (one-year-old) individuals were observed, it is necessary to continue the research with reference to older animals (two-year-old foxes).
EN
A highly sensitive and specific real-time PCR assay was used for detection and quantitation of equine herpesvirus type 1 (EHV-1) in the different internal organs of aborted fetuses. Tissue samples from 23 aborted fetuses submitted to the Department of Virology of the National Veterinary Research Institute in Pulawy between 2012 and 2013 were used for testing. Total DNA was extracted using a phenol-chloroform-isoamyl alcohol standard protocol. A real-time PCR with forward and reverse primers encompassing a highly conserved region encoding viral glycoprotein B was adapted for diagnosis of EHV-1 infection. The detection limit of the assay was shown to be 6.0x100 of viral DNA copies and the obtained standard curve exhibited a linear range from 100 to 107 molecules. Sixteen out of twenty three aborted fetuses (69.5%) were positive for EHV-1 in real- time PCR. The highest EHV-1 DNA load was obtained for liver (mean Ct value: 15.7) and lung (18.2) samples, while the lowest was in the thymus (29.6) and placenta (28.4).
EN
We analysed 7 territories of the nightjar located in the Puławy Forest District (eastern Poland). Density of the breeding population on the studied area was 0.88 territory/km2. The smallest territory covered 4.5 ha, while the biggest one – 18.7 ha (average 13 ha). Birds were found in a loose birch and pine forest, in the plantation and nearby the working railway. Significant differences in open area contribution and in share of undergrowth and brushwood were found between analysed territories and random patches. Greater biodiversity was found in places chosen by birds.
EN
The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh - measurement A; equilibrated - measurement B; frozen/thawed - measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm3, and ejaculate volume averaged 1.6 cm3. Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×103 U/l) and lowest activity of acrosin inhibitors (4.55×103 U/l). After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.
PL
Raport podsumowuje wyniki zebrane w Kartotece Rzadkich Ptaków dotyczące gatunków lęgowych w Polsce w roku 2019. Scharakteryzowano populacje lęgowe 32 rzadkich taksonów (30 gatunków i 2 podgatunków), w tym ich liczebność, rozmieszczenie i trendy. Zróżnicowane okresy do oceny trendów podano w przeglądzie gatunków. Wysoką lub rekordową liczebność odnotowano dla: łabędzia krzykliwego Cygnus cygnus (190 par), gęsiówki egipskiej Alopochen aegyptiaca (27 p.), hełmiatki Netta rufina (52 p.), podgorzałki Aythya nyroca (131 p.), mandarynki Aix galericulata (96 p.), dubelta Gallinago media (345 samców), ślepowrona Nycticorax nycticorax (1 293 p.), czapli białej Ardea alba (378 p.), puszczyka mszarnego Strix nebulosa (10 p.), sokoła wędrownego Falco peregrinus (49 p.) i wodniczki Acrocephalus paludicola (4 500 s.). Trend spadkowy dotyczył m.in.: mewy czarnogłowej Ichthyaetus melanocephalus (42 p.), rybołowa Pandion haliaaetus (27 p.) i kraski Coracias garrulus (13 p.). Populacje ostrygojada Haematopus ostralegus (26 p.), kulika wielkiego Numenius arquata (167 p.), orlika grubodziobego Clanga clanga (17 p.; w tym 8 czystych par) i orła przedniego Aquila chrysaetos (33 p.) utrzymywały się na stabilnym poziomie. Populacja rybitwy czubatej Thalasseus sandvicensis (130 p.) poniosła całkowite straty lęgowe. Poza główną ostoją (Bagna Biebrzańskie) odnotowano w kraju 35 p. pliszki cytrynowej Motacilla citreola. Następujące taksony nie przekroczyły liczby 5 p.: bernikla kanadyjska Branta canadensis (1 p.), szczudłak Himantopus himantopus (1 p.), gadożer Circaetus gallicus (3 p.), uszatka błotna Asio flammeus (5 p.), „srokosz stepowy” Lanius excubitor homeyeri (1 p.), czarnowron Corvus corone (3 p. mieszane z wrona siwą C. cornix), pomurnik Tichodroma muraria (min. 1 p.) i „pliszka tundrowa” M. flava thunbergi (4 p.). Nie wykryto lęgów szablodzioba Recurvirostra avosetta, aleksandretty obrożnej Psittacula krameri, wójcika Phylloscopus trochiloides i droździka Turdus iliacus, gniazdujących w poprzednich latach.
EN
The report of the Rare Birds Panel presents abundance estimates for 32 rare breeding taxa (30 species and 2 subspecies) in Poland for 2019. The high or maximum numbers were recorded for: the Whooper Swan Cygnus cygnus (190 pairs), Egyptian Goose Alopochen aegyptiaca (27 p.), Red-crested Pochard Netta rufina (52 p.), Ferruginous Duck Aythya nyroca (131 p.), Mandarin Duck Aix galericulata (96 p.), Great Snipe Gallinago media (345 males), Black-crowned Night Heron Nycticorax nycticorax (1293 p.), Great Egret Ardea alba (378 p.), Great Grey Owl Strix nebulosa (10 p.), Peregrine Falcon Falco peregrinus (49 p.) and Aquatic Warbler Acrocephalus paludicola (4500 m.). The declining trend concerned the Mediterranean Gull Ichthyaetus melanocephalus (42 p.), Western Osprey Pandion haliaaetus (27 p.) and European Roller Coracias garrulus (13 p.). The population of the Eurasian Oystercatcher Haematopus ostralegus (26 p.), Eurasian Curlew Numenius arquata (167 p.), Greater Spotted Eagle Clanga clanga (17 p.; including 8 pure pairs) and Golden Eagle Aquila chrysaetos (33 p.) remained stable. The population of the Sandwich Tern Thalasseus sandvicensis (130 p.) failed to produce any young. Outside the Biebrza Marshes 35 p. of the Citrine Wagtail Motacilla citreola were found. No more than 5 pairs were recorded for the Canada Goose Branta canadensis (1 p.), Black-winged Stilt Himantopus himantopus (1 p.), Short-toed Snake Eagle Circaetus gallicus (3 p.), Short-eared Owl Asio flammeus (5 p.), Homeyer’s Grey Great Shrike Lanius excubitor homeyeri (1 p.), Carrion Crow Corvus corone (3 p. mixed with Hooded Crow C. cornix), Wallcreeper Tichodroma muraria (min. 1 p.) and Grey-headed Wagtail M. flava thunbergi (4 p.). There were no breeding records of the Pied Avocet Recurvirostra avosetta, Rose-ringed Parakeet Psittacula krameri, Greenish Warbler Phylloscopus trochiloides and Redwing Turdus iliacus.
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