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A molecular approach to the study of avian DNA in bat faeces

100%

Pastor-Bevia D. , Ibanez C. , Garcia-Mudarra J. L. , Juste J.

2014

tom 16

nr 2

The molecular identification of prey in faeces is an efficient non-invasive technique to study diet which requires both a satisfactory method of DNA extraction and the design of specific primers to selectively amplify prey's DNA. In this study we evaluated and compared the efficiency of two total DNA extraction methods and five primer pairs for the molecular identification of birds from scats, in particular from the giant noctule bat (Nyctalus lasiopterus). A modified DNA stool Mini Kit of Qiagen was tested against a modified silica method with a guanidinium thiocianate (GuSCN) applied after freezing and pulverizing the samples. We also checked two published vertebrate- and bird-generalist primer pairs and three bird-specific primer pairs designed by us (two pairs targeting the cytochrome b and one the cytochrome oxidase subunit I genes) that amplified shorter DNA fragments. The results show that pulverizing the scat remains before extraction was a very important step, presumably facilitating access to the well preserved DNA located inside the rachis of the feathers. The combination of our bird-specific designed primers showed a higher amplification rate than the generalist primers and allowed successful bird identification from the feathers excreted by the giant noctule bat in all the scat samples analyzed, independent of the preservation method used (dried and frozen). These methodological improvements will allow not only the study of the avian diet composition of the enigmatic giant noctule, but the extension of this methodology to other bird predators such as raptors.

Taxonomy of little bent-winged bats (Miniopterus, Miniopteridae) from the African islands of Sao Tome, Grand Comoro and Madagascar, based on mtDNA

88%

Juste J. , Ferrandez A. , Fa J. E. , Masefield W. , Ibanez C.

nr 1

Due to a morphological uniformity typically shown by bent-winged bats, the taxonomic recognition of species and subspecies within the sole genus Miniopterus has been much questioned and revised. The situation and definition of the African species M. minor is particularly confused. This species is known from scattered and discontinuous records on both mainland coasts, Madagascar, São Tomé and Grand Comoro islands. The island forms have been included either within M. minor or considered as endemic species. To clarify their taxonomy, we compare mitochondrial DNA sequences of all the island forms with other related African Miniopterus. The genetic distances found in this study support a taxonomic recognition of the island forms at species level and the phylogenetic reconstructions based on these data suggest that the M. minor, as considered traditionally, is not a monophyletic group. The morphological similarities between the Miniopterus from São Tomé (West Africa) and Grand Comoro (East Africa) may reflect convergent evolution rather than a common ancestry.