Wyniki wyszukiwania - Biblioteka Nauki

1

Probnik 'Dermomedium' do oceny liczby drobnoustrojow z powierzchni uzytkowych i skory

100%

Muszynski Z. , Dlugaszewska J.

Medycyna Doświadczalna i Mikrobiologia

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1994

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tom 46

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nr 1-2

47-50

2

Septo-Clean nowy srodek dezynfekcyjny i antyseptyczny

100%

Muszynski Z. , Dlugaszewska J.

Medycyna Doświadczalna i Mikrobiologia

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1994

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tom 46

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nr 1-2

91-94

3

Konserwacja przeciwdrobnoustrojowa niektorych kosmetykow

100%

Muszynski Z. , Dlugaszewska J.

Medycyna Doświadczalna i Mikrobiologia

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1994

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tom 46

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nr 1-2

87-90

4

Wrazliwosc na antybiotyki bakterii izolowanych z moczu chorych leczonych w oddziale dzieciecym

80%

Justynska E. , Powarzynska A. , Dlugaszewska J.

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nr 3

231-236

PL

Przeprowadzono analizę wyników badań moczu dzieci hospitalizowanych w oddziale pediatrycznym w latach 2006 - 2008. Najczęściej izolowano pałeczki E. coli, znacznie rzadziej E.faecalis, P. aeruginosa, P. mirabilis. Wyosobnione drobnoustroje wykazywały zróżnicowaną wrażliwość na badane antybiotyki. Niepokojącym jest występowanie pałeczek Gram-ujemnych wytwarzających ESBL oraz enterokoków HLAR i VRE.

EN

The study was an analysis of the frequency of urine bacterial isolation in hospitalized children as well as an evaluation of their susceptibility to antibiotics used in urinary tract infections (UTI). The analysis focused on microbiological urine tests carried out between January 2006 and December 2008. Altogether, 311 strains were obtained, of which E. coli (50,8%) and E.faecalis (13,5%) were the most frequently isolates. The highest percentage of Enterobacteriaceae were sensitive to ceftazidime (92%); to a lesser degree to amoxicillin/clavulanic acid (85%), to trimethoprim/sulfamethoxazole (84%), to nitrofurantoin (82%), to cefuroxime (81%), to cefalotin (66%) whereas only 24% were sensitive to ampicillin. ESBLs were produced by 8% of all Enterobacteriaceae strains. P. aeruginosa strains were totally sensitive to ceftazidime; over 90% - to piperacillin and aminoglycosides, and 77% to carbenicillin. Staphylococci manifested 100% sensitivity to nitrofurantoin. Only 20% of S. aureus were sensitive to trimethoprim/sulfamethoxazole and to trimethoprim; in the case of S. epidermidis: 83% and 67% respectively. No resistant strains were found among S. agalactiae and E.faecalis. E.faecium strains, in turn, were resistant to ampicillin and often to nitrofurantoin (64%), to vancomycin (VanB; 45%) and to high aminoglycoside concentrations (HLAR; 45%).

5

Assessment of drug sceptibility of Pseudomonas aeruginosa strains isolated from the pharmaceutical production environment

80%

Ratajczak M. , Kaminska D. , Dlugaszewska J. , Gajecka M.

Postępy Mikrobiologii. Suplement

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2017

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tom 56

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nr 3

6

Influence of some microorganisms on viability of human peritoneal mesothellal cells

70%

Dlugaszewska J. , Ratajczak M. , Slupska I. , Kaminska D. , Gajecka M.

Postępy Mikrobiologii. Suplement

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2017

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tom 56

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nr 3

7

Micropropagation of Eryngium campestre L. via shoot culture provides valuable uniform plant material with enhanced content of phenolic acids and antimicrobial activity

61%

Kikowska M. , Thiem B. , Sliwinska E. , Rewers M. , Kowalczyk M. , Stochmal A. , Dlugaszewska J.

Acta Biologica Cracoviensia. Series Botanica

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2016

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tom 58

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nr 1

EN

An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro- and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg lˉ¹ benzyladenine (BA) and 0.1 mg lˉ¹ indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.

8

In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species

61%

Kikowska M. , Dlugaszewska J. , Kubicka M. M. , Kedziora I. , Budzianowski J. , Thiem B.

Herba Polonica

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2016

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tom 62

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nr 2

EN

Introduction: Due to increasing resistance against antibiotics and antifungal agents, crude plant extracts, fractions, and isolated pure compounds became a new interest as antimicrobial agents. Objectives: The antimicrobial activity of methanolic extracts and fractions of Eryngium planum L., E. campestre L., and E. maritimum L. was evaluated against selected bacteria, yeast and mould, and compared in tested Eryngium species and in their organs. Methods: The antimicrobial activity was studied with use of broth microdilution method. The antibacterial (Staphylococcus aureus, Pseudomonas aeruginosa) and antifungal (Candida albicans, Aspergillus niger) activity of selected extracts and fractions compared with the reference substance was expressed by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal/Fungicidal Concentration (MBC/MFC).The extract and fraction compounds were identified on the basis of TLC examination. Results: The saponin-phenolic acid fractions of E. maritimum and E. planum and a saponin fraction of E. planum showed the highest activity against S. aureus (MIC = 1–2.5 mg·ml-1). The growth of C. albicans was inhibited by methanolic extract of E. planum cell suspension culture (MIC = 7.8 mg·ml-1). Conclusion: The antimicrobial activity depends on the Eryngium species, tested biomass, and microorganism.

PL

Wstęp: Z powodu rosnącej oporności na antybiotyki i środki przeciwgrzybicze rośnie zainteresowanie ekstraktami roślinnymi, frakcjami i wyizolowanymi czystymi związkami jako środkami przeciwdrobnoustrojowymi. Cel: Badano aktywność przeciwdrobnoustrojową ekstraktów metanolowych i frakcji Eryngium planum L., E. campestre L. i E. maritimum L. w stosunku do wybranych bakterii, drożdżaka i grzyba pleśniowego oraz porównywano badane gatunki Eryngium i ich organy. Metody: Aktywność przeciwdrobnoustrojową badano z zastosowaniem metody seryjnych rozcieńczeń w bulionie. Aktywność przeciwbakteryjna (Staphylococcus aureus, Pseudomonas aeruginosa) i przeciwgrzybicza (Candida albicans, Aspergillus niger) wybranych ekstraktów i frakcji, w porównaniu z substancją referencyjną, wyrażona została za pomocą minimalnego stężenia hamującego (MIC) i minimalnego stężenia bakterio- lub grzybobójczego (MBC/MFC). Wyniki: Frakcje saponinowo-fenolokwasowe E. maritimum i E. planum oraz frakcja saponinowa E. planum wykazały najwyższą aktywność wobec S. aureus (MIC = 1–2,5 mg∙ml -1). Wzrost C. albicans był hamowany przez metanolowy ekstrakt zawiesiny komórkowej E. planum (MIC = 7,8 mg∙ml -1). Wnioski: Aktywność przeciwdrobnoustrojowa zależy od gatunku Eryngium, testowanej biomasy i mikroorganizmu.

9

Evaluation of antiamoebic and antimicrobial activities in vitro of Chaenomeles japonica (Thunb.) Lindl. ex Spach extracts

51%

Klikowska M. , Derda M. , Thiem B. , Wlodarczyk A. , Dlugaszewska J. , Stochmal A. , Zuchowski J. , Hadas E.

Acta Biologica Cracoviensia. Series Botanica

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2019

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tom 61

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nr 2