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1

Trimethilin-induced plastic neuronal changes in rat hippocampus are accompanied be astrocytic trophic activity

100%

Koczyk D. , Skup M. , Zaremba M. , Oderfeld-Nowak B.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 1

237-241

EN

Partial deafferentation of the hippocampus due to trymethyltin (TMT) intoxication has been reported to induce plastic rearrangements of neuronal elements but the factors that direct these responses are unknown. To assess the possible involvement of nerve growth factor (NGF) in the phenomenon we evaluated the presumable changes in the expression pattern of NGF immunoreactivity (NGF-IR) in rat hippocampus 21 days after administration of TMT (8 mg/kg, i.p.) when reactive changes are fully developed. Immunolabelling for TrkA known to mediate biological effects of NGF and for GFAP to identify astroglial cells as a one of presumed source of postinjury produced factors was carried out on adjacent sections to establish the relation between expression of these proteins. In control hippocampus NGF-IR and TrkA-IR were localized in neurons and/or nueropil. After exposure to TMT remarkable non-neuronal expression of both proteins was observed. The distribution pattern of NGF, TrkA and GFAP overlapped suggesting that reactive asrtocytes may not only produce NGF but also may become responsive to this neurotrophin. Zones of extensive NGF and TrkA astroglial expression corresponded to areas of axonal-dendritic rearrangements reported earlier. That data suggest that astroglia-derived trophic activity may be involved in neuronal plastic events associated with treatment with TMT.

2

Difluoromethylornithine counteracts lesion-induced astrogliosis in rat hippocampus: enhancement of inhibitory effect by combined treatment with GM1 ganglioside

100%

Jaglinski W. , Skup M. , Zaremba M. , Oderfeld-Nowak B.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 2

549-553

EN

The effect of difluoromethylornithine (DFMO), an irrevesible inhibitor of ornithine decarboxylase (ODC), the rate limiting enzyme of polyamine biosynthesis, and its combined action with GM1 ganglioside, was studied on the GFAP content in a model of remote astrogliosis evoked in the hippocampus by latral fimbria transection.DFMO markedly suppressed hipocampal gliosis as measured by GFAP immunoblotting seven days postsurgery.Combined treatment with DFMO and GM1 ganglioside produced a stronger suppressive effect than DFMO.The results support the hypothesis of a causal link between lesion induced events:polyamine biosynthesis and astroglial reaction.Potentation of the inhibitory effect of DFMO by GM1 ganglioside suggest that the latter does not act through the mechanism involving ODC suppression.

3

Axonal accumulation of p75NTR and TrkA in the septum following lesion of septo-hippocampal pathways

75%

Skup M. , Bacia A. , Koczyk D. , Jeglinski W. , Zaremba M. , Oderfeld-Nowak B.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 2

515-525

EN

Septal cholinergic neurones depend on trophic support by nerve growth factor (NGF) which can rescue them from injury-induced degeneration.Since NGF exerts its effects via p75NTR and TrkA receptors coexpressed in vast majority of these neurones and down-regulated without NGF treatment after injury.In this study we aimed to examine how does the lesion to the cholonergic tracts affect distribution of both type of receptor proteins in damaged fibres.Early changes (two and seven days) were examined immunocytochemically within the septum and supracallosal stria after unilateral lesion to the supracollosal area and cingulum transecting some septal cholinergic efferents.

4

Interleukin 6, tumor necrosis factor alpha and their soluble receptors in the blood serum of patients with denture stomatitis and fungal infection

75%

Pietruski J. K. , Pietruska M. D. , Jablonska E. , Sacha P. , Zaremba M. , Stokowska W.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

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nr 2

101-105

EN

Determinations of the blood serum levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and their soluble receptors (sIL-6R, sTNFR) in denture stomatitis patients (DS) were performed. Serum levels of interleukins and their soluble receptors were measured using the ELISA method. In all examined patients mycological diagnostics were conducted using API 20C AUX stripe tests and an automatic ATB machine. Results were compared with those of healthy denture weares (D), and controls (C). In DS patients, yeasts were isolated in 90. 9%, in D in 66. 7% of cases. The most often isolated species in both groups was Candida albicans. Mean concentrations of IL-6 and TNF-alpha were statistically significantly higher in D and D groups compared to controls. Mean concentrations of sIL-6R were similar in all groups; however, concentrations of sTNFR in both DS and D groups were significantly lower compared to controls. There were no correlations found between values of IL-6 and TNF-alpha nor between examined interleukins and their soluble receptors.