Wyniki wyszukiwania - Biblioteka Nauki

1

Woffia arrhiza - najmniejsza roslina naczyniowa o najwiekszych mozliwosciach adaptacji i zastosowania

100%

Czerpak R. , Piotrowska A.

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nr 2-3

241-250

2

Cytokininy, ich aktywnosc biochemiczna w procesach podzialow, starzenia sie i apoptozy komorek ludzkich i zwierzecych

100%

Jablonska-Trypuc A. , Czerpak R.

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nr 1

135-154

3

Stimulatory effect of auxins and cytokinins on carotenes, with differential effects on xanthophylls in the green alga Chlorella pyrenoidosa Chick.

100%

Czerpak R. , Bajguz A.

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tom 66

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nr 1

41-46

4

Cellular response of light/dark-grown green alga Chlorella vulgaris Beijerinck (Chlorophyceae) to exogenous adenine- and phenylurea-type ctokinins

100%

Piotrowska A. , Czerpak R.

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tom 31

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nr 3

EN

Treatment exposed to light Chlorella vulgaris Beijerinck (Chlorophyceae) with adenine- (BA, Kin, Z) and phenylurea-type (DPU) cytokinins effects positively on alga viability by 1.5- to twofold increase in cell number, chlorophylls, carotenoids, monosaccharides and glycolate content as well as NADH-dependent hydroxypyruvate reducing enzyme activity (NADH-HPR) extensively involved in carbon metabolism. Cytokinins enhance nitrogen assimilation by stimulation of NADH-dependent glutamate dehydrogenase (NADH-GDH) aminating activity finally leading to higher protein level and its secretion as well as polypeptide accumulation in the range of molecular weight 12–195 kDa. In the dark, cytokinins mimic the regulatory effect of light upon algal cell division, metabolite content and stimulate carbon recycling for Calvin cycle reactions by enhancing of light-dependent NADH-HPR activity. The delaying of protein degradation and stimulation of their secretion to environment, triggering polypeptide accumulation and twofold higher NADH-GDH activity catalysing amino acids biosynthesis are observed in the dark-grown microalgae in response to cytokinins. Chlorella vulgaris exhibits sensitivity on cytokinins in the following order of their stimulating properties: DPU > Z > Kin > BA in both light and dark conditions. Understanding of cytokinin role in lower plants under different light conditions could be a step toward the elucidation of the evolution of hormone regulation and their action at molecular level.

5

The effect of beta-estradiol and corticosteroids on chlorophylls and carotenoids content in Wolffia arrhiza [L.] Wimm. [Lemnaceae] growing in municipal Bialystok tap water

100%

Czerpak R. , Szamrej I. K.

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nr 6

EN

It is well known that many steroid compounds, mainly from large stock-raising farms, more frequently end up in rural or communal sewage systems. It is also known that the duckweed family (Lemnaceae), especially Wolffia arrhiza, is more and more commonly used in the biotechnology of purifying the above-mentioned sewage systems due to its heterotrophic and detoxication ability, as well as its ease of adoption to unfavorable environmental conditions. Therefore, our research analyzes the influence of beta-estradiol and chemically and functionally diversified corticosteroids: cortisone, cortisole (glucocorticoids), "-deoxycorticosterone (miner- alocorticoids) and prednisolone (chemical derivative of hydrocortisone) on chlorophylls and carotenoids content in photoautotrophic Wolffia arrhiza (Lemnaceae), growing in municipal Białystok tap water (rich in minerals but poor in organic components). From the applied steroid hormones in optimal concentration of 10-6M p-es- tradiol caused the strongest stimulatory effect on photosynthetic pigments, a little less strong - cortisone, slight stimulative - cortisole, and weak "-deoxycorticosterone. Prednisolone showed a weak inhibitory influence on all types of chlorophylls and carotenoids in comparison with the control culture without exogenous hormones. Applied steroid hormones had a weak stimulative influence over chlorophylls a and b in Wolffia; the strongest was p-estradiol between the 5th and the 10th day of cultivation, in the range of 116.5-121.3% in comparison to the control value (100%). The researched steroids had a much stronger influence on carotenoid content, especially p-carotene, alloxanthin (oxygen - poor xanthophylls) and violaxanthin (oxygen - rich xanthophylls). Under the influence of beta-estradiol the amount of p-carotene rose by the maximum 160.6%, alloxanthin by 187.9% and violaxanthin by 154.3% in comparison to the control. Our research results demonstrated that p-estradiol and - from applied corticosteroids - cortisone and hydrocorticosterone, had more stimulatory influence on carotenoid content in Wolffia arrhiza, but less stimulatory effect on unicellular Chlorella vulgaris.

6

The effect of sodium amidotrizoate on the growth and content of various metabolites in the green alga Chlorella vulgaris Beijerinck

80%

Pietryczuk A. , Czerpak R. , Popławska B. , Wolski T.

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tom Vol. 39, No.1

61-73

EN

The aim of this work is to detect how 3,5-diacetamido-2,4,6-triiodosodium benzoate (sodium amidotrizoate), a synthetic derivative of the anti-auxin triiodonezoic acid (TIBA), influences the growth and content of primary metabolites in the green alga Chlorella vulgaris Beijerinck. It strongly suppressed cell number by 6–20%, chlorophyll a by 6–14%, chlorophyll b by 20–24%, total carotenoids by 7–13% and monosaccharides by 7–8% at the concentration of 10^-4 M, in comparison to the control. On the other hand, sodium amidotrizoate applied at lower concentrations (10^-7 – 10^-6 M) acted as a weak stimulator of analyzed biochemical compounds in algal cells. No statistically significant effect was detected with the application of sodium amidotrizoate at a concentration of 10^-8 M. It seems that sodium amidotrizoate plays an important role in algal growth and development and probably possesses anti-auxin activity, like TIBA.

7

Metabolic activity of salicylic acid in Wolffia Arrhiza [L.] Wimm. growing in different trophical conditions

80%

Czerpak R. , Piotrowska A. , Krotke A.

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nr 4

445-452

8

Qualitative analysis of carotenoids in particular parts of body of males and females of Euphausia superba Dana (Crustacea)

80%

Czerpak R. , Jackowska H. , Mical A.

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nr 2-3

9

Investigation on carotenoids in lichens. V. Lichens from Antarctica

80%

Czeczuga B. , Gutkowski R. , Czerpak R.

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nr 3

10

Jasmonic acid affects changes in the growth and some components content in alga Chlorella vulgaris

80%

Czerpak R. , Piotrowska A. , Szulecka K.

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nr 3

EN

The present study was undertaken to test the influence of exogenous applied jasmonic acid upon the growth and changes in some metabo-ites levels in the cells of green alga Chlorella vulgaris Beijerinck (Chlorophyceae). It was found, that JA in algal cells acted in a concentration-dependent manner. Treatment with JA at high concentrations range of 10⁻⁵-10⁻⁴ M, resulted in the decrease in cell number and reduction of major photosynthetic pigments, monosaccharides, soluble cellular and extracellular proteins levels as well as decrease in pH of the medium. In contrast to 10⁻⁵ and 10⁻⁴ M JA, this phytohormone applied at 10⁻⁸-10⁻⁶ M induced the increase in cell number, photosynthetic pigments and monosaccharides contents, significant accumulation and extracellular secretion of soluble proteins over control and neutralization of the medium. Quantitative changes in polypeptide pattern of total cellular proteins after treatment with the optimal concentration of 10⁻⁷ M JA on the 7th day of cultivation as analyzed by SDS-PAGE, was also observed. JA induced synthesis de novo of15 specific polypeptides with molecular weight 334-16 kDa which weren’t detected in the control. The data suggest that JA plays a important role in algal growth and development.

11

Występowanie i biosynteza karotenoidów u bakterii

80%

Czerpak R. , Czeczuga B.

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nr 2

12

Activity of salicylic acid on the growth and biochemism of Chlorella vulgaris Beijerinck

70%

Czerpak R. , Bajguz A. , Gromek M. , Kozlowska G. , Nowak I.

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tom 24

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nr 1