Wyniki wyszukiwania - Biblioteka Nauki

1

Czynnik wzrostu nerwow - jego ekspresja i udzial w funkcjonowaniu narzadow rodnych samic

100%

Jana B. , Kozlowska A.

|

nr 04B

520-524

EN

The nerve growth factor (NGF) is a polypeptide belonging to the family of trophic factors, influencing not only the population of neurons of both the central and peripheral nervous system, but also cells of the immunological and endocrine systems. The expression of NGF and/or its two types of membrane receptors (NTRK1 and TFRSF1B) was found in cells of ovaries, uterus, oviduct, fetal membranes and placenta. NGF is an important element of the regulation of the ovary functioning in sexually immature and mature females. NGF contributes to the development of the innervation of ovarian structures, stimulates proliferation of steroidogenic cells and synthesis of steroids, as well as participates in the maturation of oocytes and in ovulatory process. Moreover, NGF and neurotransmitters released by adrenergic fibres can play an important role in the formation and/or the course of polycystic ovary syndrome. During the estrous cycle, besides pregnancy NGF stimulates proliferation of uterine cells which lead to the increase of the mass of the uterus. Decreased in the middle and late period of the pregnancy, the expression of this factor in the uterus contributes to neurodegenerative changes whose effect is the reduction of contractions of the uterus. In turn the augmentation of NGF production after parturition leads to the restoration of innervation in this organ. The implication of NGF and its receptors in the functioning of the oviduct, fetal membranes and placenta has not yet been recognized.

2

The cannulation of the caudal caval vein through the femoral vein in the pig for endocrine research

100%

Kucharski J. , Jana B.

|

nr 2

3

Wplyw zearalenonu na funkcje rozrodcze zwierzat

100%

Jana B. , Skwarski R.

|

tom 54

|

nr 10

667-670

4

Wplyw cytokin na hormonalna regulacje procesow rozrodu

100%

Jana B. , Stepien A.

|

tom 23

|

nr 4

513-528

5

The influence of the ozone and vitamin C on steroidogenic activity of rat testes

80%

Jedlinska-Krakowska M. , Jana B. , Kozlowska A. , Jakubowski K.

|

tom 22

|

nr 3

545-555

EN

The study was performed on sexually mature male rats divided randomly into six groups: I – control rats; II and III – ozonized rats and receiving im injections of vitamin C at doses, respectively, 20 and 40 mg/rat, and IV and V – rats did not expose to ozone, treated with mentioned above doses of vitamin C, VI – animals only ozonized, without vitamin injections. On the ground obtained results can ascertain that oxidation stress caused by ozone disturbs process of steroidogenesis in testes leading to a decrease in the content of enzymes – P450scc and 3β-HSD, and consequently the concentrations of testosterone (T) and estradiol-17β (E₂) in gonadal tissue (in spite the elevated content P450arom) Vitamin C distinctly increases the 3β-HSD content in testes restoring the physiological T and E₂ concentrations.

PL

Doświadczenie przeprowadzono na dojrzałych płciowo samcach szczurów podzielonych na 6 grup: I – zwierzęta kontrolne; II i III – zwierzęta ozonowane i otrzymujące domięśniowe iniekcje witaminy C w dawkach odpowiednio 20 i 40 mg/szczura oraz IV i V – zwierzęta nieozonowane otrzymujące w/w dawki witaminy C, VI – szczury ozonowane bez iniekcji witamin. Na podstawie uzyskanych wyników można stwierdzić, że stres oksydacyjny powodowany przez ozon zaburza proces steroidogenezy w jądrach, obniżając zawartość enzymów P450scc i 3β -HSD, a w konsekwencji koncentrację testosteronu (T) i 17β -estradiolu (E₂) w tkance gonad (pomimo podwyższonej zawartości P450arom). Witamina C wyraźnie zwiększa zawartość dehydrogenazy 3β-HSD, przywracając fizjologiczny poziom T i E₂.

6

Involvement of nitric oxide in inflammation of ovaries in gilts

80%

Jana B. , Andronowska A. , Kucharski J.

|

2002

|

tom 02

|

nr 1

7

Nitric oxide mediates an inflammatory effect of Escherichia coli in the porcine uterus

80%

Jana B. , Andronowska A. , Kucharski J.

|

nr 4

EN

The purpose of this study was to determine whether inflammatory changes in uterus caused by Escherichia coli are associated with changes in nitric oxide (NO) production. Therefore, the activity of NADPH-diaphorase (NADPH-d), a histochemical marker for nitric oxide synthase (NOS), localization of inducible isoform of NOS (iNOS) and tissue content of nitrite were studied in the uterine structures after inoculation of Escherichia coli into the uterus in gilts. Twelve sexually matured gilts with controlled estrous cycle were used. The animals were laparotomized on the 2nd day of the estrous cycle and polyvinyl cannulas were inserted into the uterine horns to infuse saline or Escherichia coli. In the group I (control; n=6), 25 ml of saline was infused into each uterine horn on the 4,h day of the estrous cycle. At the same time, 25 ml of Escherichia coli (strain 025:K23/α/:Hl) suspension, containing 107 colony forming units/ml was inoculated into each uterine horn of the treated gilts (group II; n=6). The middle part of the uterine horn was collected on the 14th day of the next estrous cycle immediately after slaughter. Cryostat sections from paraformaldehyde fixed tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. Optical density was assessed to evaluate the intensity of the histochemical reaction. Nitrite content was measured spectrophotometrically. In the Escherichia coli-treated gilts, the activitý of NADPH-d in the luminal epithelium and in external parts of excretory ducts of uterine glands was higher (P < 0.001) as compared to that in the control animals. In the secretory part of the uterine glands the activity of this enzyme was similar in both groups of the gilts. In the gilts that received Escherichia coli, the histochemical reaction of NADPH-d in endometrial blood vessels was stronger than that found in the control animals. Immunoreactivity for iNOS in the luminal epithelium, in external parts of excretory ducts of uterine glands and in vascular endothelial cells was stronger in the Escherichia coli-treated gilts as compared to that observed in the control animals. Only weak or no immunoreactivity was found in the secretory part of the uterine glands in both groups of the gilts. After Escherichia coli inoculation, nitrite content in the uterine tissues was higher (P < 0.05) than that determined in the controls. Our study has revealed that infusion of Escherichia coli into the porcine uterus induces the activity of NADPH-d, iNOS and increases the tissue content of nitrite in this organ. The data obtained indicate that NO can mediate an inflammatory effect of Escherichia coli in the uterus.

8

Immunoreactivity of iNOS in porcine uterus after infusions of Escherichia coli endotoxin

80%

Jana B. , Andronowska A. , Kucharski J.

|

2001

|

tom 39

|

nr 2

9

Cholinergic innervation of cystic porcine ovaries

80%

Kozlowska A. , Wojtkiewicz J. , Majewski M. , Jana B.

|

nr 04B

EN

The aim of the study was to examine the changes in the density of VAChT (marker of acetylcholine present)-, NPY-, VIP-, SOM-, SP- and nNOS-immunoreactive (IR) nerve terminals and co-localization of VAChT with the above-mentioned neurotransmitters after the occurrence of dexamethasone (DXM)-induced ovarian cysts in gilts. DXM administration led to an increase in the density of VAChT/SP-, VAChT/nNOS- and NPY-IR nerve terminals around the cystic walls. In DXM-treated animals an elevated number of VAChT- and SP-IR nerve endings was found close to the tertiary follicles. Moreover, in the gilts receiving DXM the density of NPY-IR nerve endings (that simultaneously co-localized VAChT) was high near the interstitial gland. An increase in the number of VAChT/SP- and VIP-IR nerve fibers around the medullar arteries (A) was observed in cystic ovaries, while the number of VAChT-IR nerve endings near the cortical A was lowered after DXM application. Furthermore, nerve fibers containing VAChT were absent around veins in the whole ovary of DXM-treated animals. After DXM injections, an increase in the number of VAChT/SP- and VAChT/nNOS-IR nerve endings in the cortical, as well as VIP- and nNOS-IR (co-existing with VAChT), nerve terminals in the medullar part of the autonomic ground plexus (GP) was present. However, the administration of DXM led to a drop in the density of SOM-positive nerve endings (also VAChT-IR) in the medullar subdivision of the GP. The present study shows that in the porcine ovaries with DXM induced cysts the pattern of cholinergic innervation, as well as the co-localization of VAChT and NPY, VIP, SOM, SP or nNOS, were changed. Data obtained also suggest that acetylcholine and the above-mentioned neurotransmitters effecting the functioning (steroidogenic activity, blood flow) of the polycystic ovaries may have a significant influence on the course of this pathological status.

10

Changes in periestrous hormonal profile in blood of gilts with different thyroid function and ovarian cysts

80%

Fitko R. , Jana B. , Kucharski J. , Sobczak J.

|

nr 1

EN

The subject of this wprk was the investigation of luteinizing hormone, progesterone, testosterone and total estrogens concentration in the peripheral plasma of 56 gilts with experimentally-induced hypo- and hyperthyroidism and with ovarian cystic degeneration. The blood samples were collected from the cannulated jugular vein at 19-23 days after last natural estrus, 5-10 times daily. The experiments resulted in significant deviations in plasma hormone profile during the periestrous period. The hypothyroid gilts showed a significant increase but hyperthyroid animals a decrease in level of LH accompanied by a significant peak of estrogens on the 22nd day of cycle. The cyst-bearing gilts exhibited a decreased level of LH, an increased concentration of progesterone and a changeable content of estrogens. It may be suggested that abnormalities in hormonal pattern in experimental gilts were caused by derangement of functional interrelationships in the pituitary-thyroid-ovarian axis which influence sex hormone synthesis and release.

11

The influence of steroids on noradrenaline-mediated contractile reactivity of the superficial nasal and facial veins in cycling gilts

71%

Grzegorzewski W. J. , Muszak J. , Wasowska B. , Jana B. , Stefanczyk-Krzymowska S.

|

nr 2

EN

The nasal venous blood may be directed through the facial vein into the systemic circulation or through the frontal vein into the venous cavernous sinus of the perihypophyseal vascular complex, where hormones and pheromones permeate from the venous blood into the arterial blood supplying the brain and hypophysis. The present study was designed to determine the effect of noradrenaline (NA) on the tension of the nasal, frontal and facial veins of cycling gilts, and influence of ovarian steroid hormones on NA-mediated contractile reactivity. Additionally, the enzyme dopamine-β-hydroxylase catalysing the conversion of dopamine to noradrenaline (DβH) was immunolocalized in these vessels. Among three studied veins, the frontal proximal vein, that fulfill a key role in the supply of the nasal venous blood into the venous cavernous sinus, reacted to NA most strongly (P<0.001) and this reaction was weaker in the periestrous period than in luteal phase (P<0.001). Inversely, the reaction to NA of the facial proximal vein, that carry blood to the peripheral circulation, was stronger in the periestrous period than in luteal phase (P<0.05). P4, E2 and T significantly lowered NA-mediated tension of the frontal proximal vein during the periestrous period (P<0.001), while in the luteal phase P4 might antagonize relaxing effect of E2 to this vessel. The result suggests that supply of the nasal venous blood into the venous cavernous sinus is greater during the periestrous period than during the luteal phase. DβH was clearly expressed in the muscular layer of the isolated superficial nasal and facial veins of gilts in both studied stages of the estrous cycle. We suggest that the reactivity of the superficial veins of the nose and face to NA combined with the previously demonstrated reactivity of these veins to steroid ovarian hormones and male steroid pheromones may regulate the access of priming pheromone androstenol (resorebed in the nasal cavity) to the brain of gilts during periestrous period via humoral local destination transfer.

12

Zastosowanie Biolactinu-2 (oczyszczona prolaktyna świni) do stymulacji laktacji u loch

71%

Dusza L. , Sobczak J. , Jana B. , Murdza A. , Bluj W.

|

nr 09

EN

Biolactin-2 preparation containing 5.0 mg of porcine prolactin (pPRL) purified from porcine pituitaries was used to stimulate lactation in sows with periparturient agalactia and in healthy primiparous and multiparous sows. Immunological activity of Biolactin-2 approached that of the PRL standard. It was found that a single intramuscular injection of Biolactin-2 effectively stimulates milk ejection in sows with mastitis-metritis-agalactia syndrom (MMA). Performance of piglets treated with a single or double dose of Biolactin-2 and as well as that of healthy ones was similar. A single administration of Biolactin-2 to healthy primiparous sows on the 1st day after farrowing significantly increased daily body gains of their litter in the first two weeks of life. This effect was not observed in multiparous sows.

13

Effect of zearalenone on steroid secretion by porcine follicular cells in mono- and coculture

71%

Jakimiuk E. , Gajecka M. , Jana B. , Obremski K. , Gajecki M.

|

nr 3

EN

The objective of the study was to evaluate the impact of zearalenone (ZEA) on the production and secretion of some steroid sex hormones by granulosa cells in monoculture and granulosa cells with theca interna cells in coculture. Follicular cells were obtained from ovarian follicles of pre-pubertal gilts. They were exposed to 0.2% DMSO and ZEA (at a concentration of 0.4, 4, 40, and 400 ng/mL), for 48 h. The concentration of progesterone, testosterone, and oestradiol-17ß in the medium was determined by radioimmunoassay. It was found that both stimulating and inhibitory effect of ZEA on basal steroidogenesis in porcine ovarian follicular cells was depended on the type of cultures and concentrations of mycotoxin.

14

Denervation of the porcine ovaries performed during the early luteal phase influenced morphology and function of the gonad

61%

Jana B. , Dzienis A. , Panczyszyn J. , Rogozinska A. , Wojtkiewicz J. , Skobowiat C. , Majewski M.

|

nr 1

15

Wplyw zearalenonu na strukture jajnikow u niedojrzalych loszek

61%

Doboszynska T. , Jarczyk A. , Rogiewicz A. , Jana B. , Andronowska A. , Postek A.

|

nr 04

430-434

16

Factors determining sensitivity of prepubertal gilts to hormonal influence of zearalenone

61%

Jakimiuk E. , Gajecka M. , Jana B. , Brzuzan P. , Zielonka L. , Skorska-Wyszynska E. , Gajecki M.

|

tom 12

|

nr 1

EN

Among large husbandry animals, swine are the most predisposed to zearalenone (ZEA) intoxication, mainly because cereal is an important component of their diet. Studies performed so far (in vivo, in vitro) suggest that ZEA and its metabolites, which may appear due to ZEA biotransformation (especially α-zearalenole; α-ZOL), can modify signaling cascades of endogenous sex steroids, through either receptor or non-receptor mechanisms. Of all age groups of swine, immature gilts are particularly predisposed to zearalenone intoxication, as manifested by the occurrence of genital tract tissue dysfunction on exposure to ZEA. The intensity of the adverse effects observed at either systemic or local level in gilts, when compared to sexually mature swine females, suggest that specific age-dependent physiological conditions may exist, which determine the high sensitivity of gilts to exogenous estrogen-like compounds, including ZEA.

17

Mikotoksyny - substancje niepozadane w srodkach zywienia zwierzat

61%

Gajecki M. , Obremski K. , Zwierzchowski W. , Jana B. , Zielonka L. , Gajecka M.

|

2002

|

tom 77

|

nr 02

78-81

18

Level of zearalenone in blood serum and lesions in ovarian follicles of sexually immature gilts in the course of zearalenone micotoxicosis

41%

Zwierzchowski W. , Przybylowicz M. , Obremski K. , Zielonka L. , Skorska-Wyszynska E. , Gajecka M. , Polak M. , Jakimiuk E. , Jana B. , Rybarczyk L. , Gajecki M.

|

nr 3