Wyniki wyszukiwania - Biblioteka Nauki

1

Techniki biologii molekularnej w analityce produktow przemyslu miesnego

100%

Szweda P. , Kur J.

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nr 08-09

54

2

Molecular typing of Staphylococcus aureus isolated from cows with mastitis in the east of Poland on the basis of polymorphism of genes coding protein A and coagulase

80%

Jakubczak A. , Szweda P. , Lukaszewska K. , Kur J.

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nr 4

EN

The aim of this study was to test the diversity of a population of 82 strains of S. aureus isolated from cows with mastitis in the east of Poland. The isolates were typed by analysis of the number of repeats of 24 bp sequences in the X region of protein A (spa) gene and restriction fragment length polymorphism (RFLP) of the coagulase (coa) gene. Twelve different spa types were distinguished. Amplification of region X gave, in 79 cases, one stripe. In a scope of 100-364 bp 10 different products (genotypes) of amplification reaction were defined. For one strain two stripes were obtained and two strains did not contain the spa gene. The most prevalent strains had 10, 11 and 12 repeats of 24 bp sequences, which represented respectively 18%, 30% and 13% of all strains tested. The presence of any strain containing 4 or 9 sequence was not observed. In the case of analysis of the polymorphism of the coagulase gene, 13 different genotypes were identified. The most frequently appearing genotype is genotype C, in which case an amplification product is digested into three DNA fragments: 410, 320 and 160 bp. To this genotype belong 43 strains, which constitute 52% of the examined population. A significant improvement in discriminatory power was observed when results from both genes were analyzed simultaneously. In an analyzed group of 82 strains, 24 genotypes were isolated.

3

Detection and differentiation of E. coli serotypes carrying EAE gene

80%

Kotlowski R. , Ziomek M. , Hernik A. , Szweda P.

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nr 3A

826

4

Lizostafyna - zrodla otrzymywania i zastosowanie

80%

Szweda P. , Kotlowski R. , Kur J.

Biotechnologia

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2005

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nr 4

28-45

5

Lack of correlation between X region spa polymorphism and virulence of methicillin resistant and methicillin sensitive Staphylococcus aureus strains

80%

Kurlenda J. , Grinholc M. , Szweda P.

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nr 1

135-138

EN

Staphylococcus aureus is an etiological factor of severe infections in both hospital and ambulatory environments. As methicillin resistant Staphylococcus aureus strains spread quickly across healthcare centers resulting in life-threatening infections with increased mortality, they are considered more virulent than MSSA strains. Protein A, encoded by the spa gene, is one of the virulence factors involved in the staphylococcal pathogenesis. It has been suggested that the number of 24-bp tandem repeat units along the X region of the spa gene correlates with the virulence level of the strains. The current work analyzed the relationships between the virulence of MRSA and MSSA strains with region X polymorphism. No obvious correlation was observed.

6

Biofilm production and presence of ica and bap genes in Staphylococcus aureus strains isolated from cows with mastitis in the Eastern Poland

71%

Szweda P. , Schielmann M. , Milewski S. , Frankowska A. , Jakubczak A.

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tom 61

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nr 1

EN

The aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo Red plate assay was found to be irreproducible and very subjective. More objective results were obtained using the spectrophotometric, microtiter plate assay. Most of the isolates, namely 76/132 (57.6 %) were classified as biofilm producers depending on the value of absorbance in the microtiter plate test. All of the isolates tested were found to possess both icaA and icaD genes, while the bap gene was absent in all strains.

7

Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

61%

Wolska K. , Szweda P. , Lada K. , Rytel E. , Gucwa K. , Kot B. , Piechota M.

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8

In vitro biodegradation of bacterial nanocellulose under conditions simulating human plasma in the presence of selected pathogenic microorganisms

61%

Dederko P. , Malinowska-Pańczyk E. , Staroszczyk H. , Sinkiewicz I. , Szweda P. , Siondalski P.

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tom T. 63, nr 5

372--380

EN

The biodegradability of bacterial nanocellulose (BNC) was assessed based on a change in its properties under conditions simulating human plasma in the presence or absence of the pathogens Staphylococcus aureus, Candida albicans and Aspergillus fumigatus. It was shown that the dry mass of BNC did not change during 6 months of incubation, except for samples stored in the presence of moulds, where the dry mass reduced by 40%. The wet mass of all BNC samples increased after 2 months of incubation. Under these conditions, the population number of microorganisms grew about 2 log cycles during the first month and maintained this level for 6 months of storage. After 1 month of storage in sterile fluids, and in the presence of bacteria or fungi, the tensile strength of BNC decreased by 60% or 70%, respectively.

PL

Oceniano podatność na biodegradację nanocelulozy bakteryjnej (BNC) na podstawie zmiany jej właściwości w warunkach symulujących osocze ludzkie w obecności lub nieobecności patogenów Staphylococcus aureus, Candida albicans i Aspergillus fumigatus. Wykazano, że sucha masa BNC nie zmieniła się w ciągu 6 miesięcy inkubacji. Wyjątek stanowiły próbki przechowywane w obecności pleśni – ich sucha masa zmniejszyła się o 40%. Mokra masa wszystkich próbek BNC zwiększyła się już po 2 miesiącach inkubacji. W tych warunkach liczba drobnoustrojów wzrosła o ok. 2 rzędy wielkości w pierwszym miesiącu i utrzymywała się na tym poziomie przez 6 miesięcy inkubacji. Po 1 miesiącu przechowywania, zarówno w jałowych płynach, jak i w obecności bakterii lub grzybów, wytrzymałość mechaniczna BNC zmniejszyła się, odpowiednio, o 60% i 70%.