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Wpływ mikrobiomu jelitowego na układ immunologiczny

100%

Galecka M. , Pazgrat-Patan M. , Basinska A. M. , Bartnicka A.

nr 2

Qualitative and quantitative characteristics of selected bacterial groups in children with inflammatory bowel diseases

100%

Roszak D. , Galecka M. , Cichy W. , Szachta P.

Polish Journal of Microbiology

2016

tom 65

nr 2

The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selected microorganisms depends on the level of clinical activity of disease and is correlated with faecal concentration of inflammatory markers. Differences in microflora disturbance, observed in patients with Crohn’s disease and ulcerative colitis, may suggest different causes of development of both pathologies.

Mikrobiom jelita grubego a przewlekłe zapalenia zatok

100%

Galecka M. , Michalik M. , Samet A.

nr 2

Methods of analysis of gut microorganism–actual state of knowledge

88%

Ignys I. , Szachta P. , Galecka M. , Schmidt M. , Pazgrat-Patan M.

Annals of Agricultural and Environmental Medicine

2014

tom 21

nr 4

Faecalibacterium prausnitzii and Crohn's disease - is there any connection?

75%

Galecka M. , Szachta P. , Bartnicka A. , Lykowska-Szuber L. , Eder P. , Schwiertz A.

tom 62

nr 1

The aim of the study was evaluation of the correlation between the level of clinical activity of Crohn’s disease (CD) and the number of Faecalibacterium prausnitzii, total number of bacteria and the concentration of selected short chain fatty acids (SCFA) in stool. 34 individuals diagnosed with Crohn’s disease participated in this study in 2011. The disease activity was determined according to the Crohn Disease Activity Index (CDAI). The number of Faecalibacterium prausnitzii and total number of bacteria were monitored by RT-PCR. The concentrations of SCFA were determined by gas chromatography. In CD patients, Faecalibacterium prausnitzii number and percentage of the total number of bacteria were greatly reduced. In patients with CD the percentage of acetate was elevated (70%), while the percentages of propionate and butyrate were significantly reduced (14.9% and 7.99%, respectively).

Beta-glucuronidase and beta-glucosidase activity in stool specimens of children with inflammatory bowel disease

75%

Mroczynska M. , Galecka M. , Szachta P. , Kamoda D. , Libudzisz Z. , Roszak D.

tom 62

nr 3

The aim of the study was to analyze the differences in the activity of β-glucuronidase and β-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation between the level of β - glucosidase activity in stool and patient’s age in the group of healthy controls, but not in the IBD group. β-glucosidase activity in IBD and healthy subjects stool specimens did not differ significantly. The activity of β-glucuronidase in children with IBD was two times lower than in the healthy group and was correlated with age in children with IBD, but not in the group of healthy ones.