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1

Dde I RFLP at the 5'region of bovine kappa-casein gene

100%

Kaminski S.

Journal of Applied Genetics

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1996

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tom 37

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nr 2

173-178

EN

The bovine kappa-casein (CASK) gene is known as a potential quantitative trait locus in dairy cattle breeding.However, the molecular basis of the effect of the CASK allele B on different milk properties remains unclear.In this report, a 214 bp fragment of the 5' untranslated region of CASK gene containing 5 potential consensus sequences for different transcription factors was PCR-amplified to find RFLPs.A Dde I RFLP was identified.IN population of 112 Bos taurus (86 cows and 26 bulls of Polish Black and White crossbred Holstein-Friesian) and 7 Bisons bonasus individuals, 7 had no recognition sites for Dd I, 23 were hetrozygous and 89 were cut completly into two fragments.

2

Associations between bovine beta-lactoglobulin polymorphism within coding and regulatory sequences and milk performance traits

63%

Kaminski S. , Zabolewicz T.

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nr 2

91-99

EN

The bovine beta-lactoglobulin (LGB) gene is considered a potential quantitative trait locus in dairy cattle breeding. In Black-and-White dairy cattle the LGB gene has two predominant alleles A and B. This can result in three possible genotypes AA, AB and BB. Moreover, within the promoter of the gene several point mutations were found. A herd of one hundred and twenty-four Black-and-White cows were genotyped for two loci: locus LGB (exon IV, alleles A and B) and locus LGB-R (SSCP polymorphism within a fragment of LGB promoter: SSCP patterns R2, R3, R1, R9). In our sample 13 AA, 58 AB and 53 BB LGB cows and 66 R2, 16 R3, 40 R1 and 2 R9 LGB-R cows were identified. A statistical analysis revealed significant associations between LGB, LGB-R genotypes as well as intragenic haplotypes LGB/LGB-R and milk protein content during the first complete lactation. Cows with AA LGB genotype, R3 LGB-R SSCP pattern and AA/R3 haplotypes had the highest protein content. These results support the hypothesis that sequence variation within the promoter of the LGB gene is probably one of the factors responsible for differences in milk protein content.

3

Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

63%

Kaminski S. , Zabolewicz T.

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nr 4

471-476

EN

A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.

4

SSCP polymorphism within 5' region of bovine lactoglobulin (LGB) gene

63%

Kaminski S. , Zabolewicz T.

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1998

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tom 39

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nr 1

97-102

EN

In the paper the detection of the SSCP polymorphism within the 5' fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5' fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named R1, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.

5

Additive effects of 19 porcine SNPs on growth rate, meat content and selection index

51%

Kaminski S. , Help H. , Suchocki T. , Szyda J.

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nr 3

235-243

EN

A total of 306 boars (108 Large White and 198 Landrace) were genotyped for 52 candidate SNPs to determine which of the polymorphisms influence growth rate, meat content and selection index. The effects of SNPs were estimated by a mixed linear model including a random additive polygenic animal effect, fixed effects of SNPs including additive, and pairwise additive-by-additive epistases, year*season of birth, breed and RYR1 genotype. In order to estimate all possible pairwise SNP combinations without overparameterising the model a stochastic approach was adopted. A total of 1 350 replications of the model were generated, each containing five randomly selected SNPs. The final estimates of the fixed effects of the model equaled an average out of the replications. The hypothesis of a nonzero effect of SNP was tested by the Wald test. Among 4 257 estimates calculated, many significant (P<0.01), but mostly minor effects (below 1 phenotypic standard deviation) were recorded. The selected SNPs will be further investigated to determine which may be used in MAS.

6

New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle

51%

Brym P. , Kaminski S. , Rusc A.

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nr 4

445-452

EN

Milk protein genes expression in cows' mammary epithelial cells is regulated mostly by the action of prolactin mediated through the STAT5A transcription factor. The STAT5A gene is a potential quantitative trait locus (QTL) and genetic marker of production traits in dairy cattle. The sequence of the bovine STAT5A gene was analysed in this study to investigate if mutations in this sequence might be responsible for quantitative variations in milk yield and composition. Ten PCR fragments representing most important functional domains of STAT5A were screened for polymorphism. Using the SSCP method a new SNP (A/G) was found, located in intron 9 at position 9501 (GenBank AJ237937). The frequencies of alleles were estimated in 186 Black-and-White cows (0.52 and 0.48 for A and G, respectively) and in 138 Jersey cows (0.58 and 0.42 for A and G, respectively). For Black-and-White cows with different STAT5A genotypes no significant associations between STAT5A genotypes and milk performance traits were found. Statistically significant differences in the first and second lactations for milk yield, fat and protein content were found in Jersey cows. Cows with the GG genotype showed the highest milk yield, while cows with genotypes AA and AG showed higher protein contents when compared to cows with the GG genotype. Interestingly, cows with genotype AG showed significantly higher protein yields in comparison to cows with the AA genotype. For fat content, cows with genotype AA showed the highest level of this trait in the 1st and 2nd lactation. Further studies are necessary to evaluate an allele substitution effect in the population of sib-families of STAT5A heterozygous bulls.

7

Effectiveness of a program aimed at the elimination of BLAD-carrier bulls from Polish Holstein-Friesian cattle

45%

Czarnik U. , Grzybowski G. , Kaminski S. , Prusak B. , Zabolewicz T.

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2007

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tom 48

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nr 4

375-377

EN

The molecular basis of BLAD is the D128G mutation of the gene coding for the CD18 subunit of beta?2 integrin. This mutation is lethal, since homozygous (BL/BL) animals die before they reach sexual maturity. In the 1990s, BLAD was the most widespread genetic disease in HF cattle worldwide. The aim of the present study was to determine the frequency of BLAD carriers among 4645 young breeding bulls in Poland in 1995?2006. The frequency of carriers of the mutated allele showed a clear decreasing trend. The highest frequency (7.9%) was recorded while implementing the BLAD control program (1995?1997). Regular monitoring has enabled a great reduction of this threat to the tested population. Today only sporadic cases of BL/TL heterozygotes are reported (ca. 0.8% in 2004?2006).