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1 Acta Physiologiae Plantarum

1 2013

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Protein changes and proteolytic degradation in red and white clover plants subjected to waterlogging

100%

Stoychev V. , Simova-Stoilova L. , Vaseva I. , Kostadinova A. , Nenkova R. , Feller U. , Demirevska K.

nr 06

Red (Trifolium pratense L., cv. ‘‘Start’’) and white clover varieties (Trifolium repens L., cv. ‘‘Debut’’ and cv. ‘‘Haifa’’) were waterlogged for 14 days and subsequently recovered for the period of 21 days. Physiological and biochemical responses of the clover varieties were distinctive, which suggested different sensitivity toward flooding. The comparative study of morphological and biochemical parameters such as stem length, leaflet area, dry weight, protein content, protein pattern and proteolytic degradation revealed prominent changes under waterlogging conditions. Protease activity in the stressed plants increased significantly, especially in red clover cv. ‘‘Start’’, which exhibited eightfold higher azocaseinolytic activity compared to the control. Changes in the protein profiles were detected by SDS-PAGE electrophoresis. The specific response of some proteins (Rubisco, Rubisco-binding protein, Rubisco activase, ClpA and ClpP protease subunits) toward the applied stress was assessed by immunoblotting. The results characterized the red clover cultivar ‘‘Start’’ as the most sensitive toward waterlogging, expressing reduced levels of Rubisco large and small subunits, high content of ClpP protease subunits and increased activity of protease isoforms.