Wyniki wyszukiwania - Biblioteka Nauki

1

Co wiemy, a czego nie wiemy o koncentracie bialka ziemniaczanego

100%

Pastuszewska B. , Tusnio A. , Taciak M.

Pasze Przemysłowe

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2007

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tom 16

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nr 04

2-5

2

Koncentrat białka ziemniaczanego w żywieniu drobiu rzeźnego

100%

Smulikowska S. , Tusnio A. , Pastuszewska B.

Polskie Drobiarstwo

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2010

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tom 18

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nr 11

3

Koncentrat białka ziemniaczanego w żywieniu zwierząt monogastrycznych

100%

Pastuszewska B. , Taciak M. , Tusnio A.

Postępy Nauk Rolniczych

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2007

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tom 59

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nr 5

4

Gut morphology of young pigs fed diets differing in standardized ileal digestible threonine and wheat gluten used as a source of non-essential amino acids

100%

Swiech E. , Barszcz M. , Tusnio A. , Taciak M.

Journal of Animal and Feed Sciences

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2016

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tom 25

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nr 3

EN

The aim of the study was to determine whether non-essential amino acids (NEAA) in form of wheat gluten (WG) have a threonine (Thr)- sparing effect on gut structure in young pigs. A two-factorial experiment was conducted on 12 groups of 6 pigs. Pigs of 12.5 kg initial body weight (BW) were fed for about 20 days diets differing in the Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible Thr · kg–1) and WG level (20.4, 40.4 and 60.4 g of WG protein · kg–1 of respective WG20, WG40 and WG60 diets). After slaughter at about 22.4 kg of BW, samples of duodenum, middle jejunum, ileum and proximal colon were taken for histological analysis. Morphology of all segments was not affected neither by Thr level nor by Thr and WG levels interaction, whereas effect of WG level differed among segments. In the duodenum, villi length and tunica mucosa thickness were decreased by WG60. In the duodenum and proximal colon, crypt depth was greater in WG20 than WG60 and/or WG40 groups, whereas in the ileum was greater in WG60 than WG20 group, and in the middle jejunum it was not affected. Villi length : crypt depth ratio was affected by WG only in the ileum, being greater in WG20 group. Myenteron was thicker in the ileum and proximal colon in WG60 than WG20 and WG40 group, respectively. The results do not evidence a sparing effect of NEAA provided by WG on Thr utilization for maintaining the proper gut structure in young pigs.

5

Koncentrat białka ziemniaczanego - cenne, ale nie do końca poznane źródło białka roślinnego Część 2. Koncentrat białka ziemniaczanego w doświadczeniach na zwierzętach

100%

Pastuszewska B. , Tusnio A. , Taciak M.

Trzoda Chlewna

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2010

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tom 48

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nr 08

6

Koncentrat białka ziemniaczanego - cenne, ale nie do końca poznane źródło białka roślinnego. Część I

100%

Pastuszewska B. , Tusnio A. , Taciak M.

Trzoda Chlewna

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2010

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tom 48

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nr 07

7

Potato dietary fibre - preliminary characterization of the properties and nutritional effects - a review

100%

Pastuszewska B. , Antushevich H. , Tusnio A. , Taciak M.

Polish Journal of Food and Nutrition Sciences

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2009

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tom 59

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nr 3

EN

A potential source of potato dietary fibre (PDF) is potato pulp, a waste product of the starch industry. The refined potato pulp (PDF preparation) contains 50-60% of DF, 10-20% of starch and 6% of protein (on dry matter basis). PDF consists of about 55% of insoluble (mainly cellulose) and 45% of soluble (mainly pectins) fraction; has low viscosity and relatively high water-holding capacity but lower fecal bulking properties than cellulose. It is intensively fermented in the large intestine, yielding a high amount of acetate and butyrate. Feeding PDF does not affect greatly morphology and morphometry of the intestinal tract. It delays the rate of passage of digesta (as compared with cellulose), reduces fat and protein digestibility in the whole digestive tract and does not affect ileal protein digestibility (as compared with cellulose and pectin). It seems to improve mineral absorption. Few experimental results do not allow to conclude whether PDF decreases blood concentration of triglycerides, total cholesterol or LDL fraction. Data on the effects of PDF on glucose and insulin levels are also scarce and insufficient.

8

The structure of small intestinal tissue in pigs as influenced by indigestible polysaccharides

100%

Tusnio A. , Swiech E. , Taciak M. , Buraczewska L.

Journal of Animal and Feed Sciences. Supplement

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2006

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tom 15

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nr 1

9

Response of young pigs to feeding potato protein and potato fibre – nutritional, physiological and biochemical parameters

100%

Tusnio A. , Pastuszewska B. , Swiech E. , Taciak M.

Journal of Animal and Feed Sciences

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2011

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tom 20

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nr 3

10

The response of rats to solanidine glycoalkaloids and trypsin inhibitor present in potato protein concentrates, and to glycoalkaloids provided by potato sprouts

88%

Tusnio A. , Pastuszewska B. , Taciak M. , Barszcz M. , Skomial J.

Journal of Animal and Feed Sciences

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2013

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tom 22

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nr 2

EN

The effects of potato protein concentrates (PPC) differing in dietary solanidine glycoalkaloids (SGA) and trypsin inhibitor activity (TI), and of potato sprouts with differing SGA levels, were studied in rats. In Experiment 1, semi-purified isoprotein diets containing casein or one of six PPC contributing from 116 to 439 mg SGA · kg–1 and from 0.13 to 0.45 mg · g–1 of TI activity, were used. Weight gain was significantly greater on the diet containing PPC with low SGA and low TI than on PPC with high SGA and moderate TI contents, and also higher than on casein. Weight gain was also slightly depressed on diets with the highest TI activity. Substitution of PPC for casein resulted in lowering the pH of caecal digesta and increasing short-chain fatty acids concentration. Feeding diets with the greatest SGA content induced depression of protein and organic matter digestibility while diets with the greatest TI activity decreased protein digestibility but did not affect pancreas weight. In Experiment 2, a commercial rat diet, supplemented with increasing amounts of potato sprout meal providing SGA in the range from 0 to 300 mg · kg–1, was used. Neither growth performance, organ weights, caecal parameters, nor enzyme activities were affected by the diet. It is postulated that the less evident response of rats to SGA in the natural ingredient than in the semi-purified diet was due to the attenuating effect of more intensive bacterial fermentation. Potato trypsin inhibitor should be considered as an antinutritional factor in PPC; interactive effects of SGA and TI are presumable.

11

The effects of pectin and rye on amino acid ileal digestibility, threonine metabolism, nitrogen retention, and morphology of the small intestine in young pigs

88%

Swiech E. , Tusnio A. , Taciak M. , Boryczka M. , Buraczewska L.

Journal of Animal and Feed Sciences

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2012

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tom 21

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nr 1

EN

The effects of supplementing cereal-based diets with 0, 40, or 80 g of pectin (P) or 270 g of rye (R) per kg on ileal digestibility of protein and amino acids (AA), and digesta viscosity in pigs of 20 kg body weight (BW), and on growth performance, nitrogen balance, small intestinal morphology, and threonine metabolism parameters in pigs of 15 kg BW were assessed. Digesta viscosity was increased more by 80 g than by 40 g P or by R. Ileal digestibility of AA and nitrogen retention were negatively affected by P, whereas growth performance was decreased by R. The effect of the supplements on intestinal morphology was variable, except for the increase in myenteron thickness by P and crypt depth by R. The number of goblet cells containing acidic mucins was decreased by 40 g P in crypts in the mid-jejunum and by 40 g P and R in villi in the ileum. Fasting and postprandial plasma levels of free threonine and of threonine dehydrogenase activity in the liver and pancreas were not affected. In conclusion, feeding P or R negatively affects ileal AA digestibility and provokes irregular changes of small intestinal morphology. These effects cannot be attributed to the increase of digesta viscosity as the main factor.

12

The effects of type of protein and fibre fermented in vitro with different pig inocula on short-chain fatty acids and amines concentrations

75%

Taciak M. , Barszcz M. , Tusnio A. , Bachanek I. , Pastuszewska B. , Skomial J.

Journal of Animal and Feed Sciences

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2015

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tom 24

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nr 3

EN

In an in vitro experiment with pig inoculum, the main and interactive effects of type of protein and fibre on the concentrations of short-chain fatty acids (SCFA) and amines as the end products of fermentation and proteolysis were determined. The effects of inoculation with colonic digesta (C) sampled post mortem or with freshly voided faeces (F) as the sources of microflora, and of 24- vs 48-h periods of incubation, were also estimated. Potato protein and casein were fermented each with cellulose, pectin, or raw potato (resistant) starch. Each set of fermentation was repeated six times. The total SCFA concentration was greater after C than F fermentation, regardless of the substrates. It was the greatest after fermentation of both proteins with potato starch and greater after fermentation of all carbohydrates with casein than with potato protein. The largest concentrations of isobutyric and isovaleric acids were found after fermentation of casein with cellulose, but only with C (P = 0.040 and P = 0.030 for interaction, respectively). Also, a butyrogenic effect of potato starch was found only after C fermentation and was greater after 48 than 24 h. The total amines concentration after C and F fermentation of potato protein with all fibres was the same, while after C fermentation of casein it was affected by the type of fibre (P = 0.001 and P = 0.000 after 24 and 48 h, respectively). It was very high with cellulose, lower with starch and the lowest with pectin. It may be concluded that in vitro bacterial proteolysis is greatly affected by the interaction of type of protein and fibre. Forty-eight-hour fermentation with colonic digesta seems to be more discriminative than 24-h fermentation and colonic digesta is a more discriminative pig inoculum than faeces.

13

Effect of the composition and autoclave sterilization of diets for laboratory animals on pellet hardness and growth performance of mice

75%

Barszcz M. , Tusnio A. , Taciak M. , Paradziej-Lukowicz J. , Molenda M. , Morawski A.

Annals of Animal Science

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2014

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tom 14

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nr 2

14

Potato sprouts as a potential source of solanidine glycoalkaloids for nutritional studies

75%

Tusnio A. , Taciak M. , Trawczynski C. , Barszcz M. , Skomial J. , Pastuszewska B.

Journal of Animal and Feed Sciences

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2013

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tom 22

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nr 4

EN

The high price of pure forms of solanidine glycoalkaloids (GA) impedes their use in studies on the effects and safety levels of GA for farm animals. An attempt was made to take advantage of the high concentration of GA in potato sprouts and to evaluate them as a potential source of these compounds for nutritional experiments. Over a period of two years, two batches of sprouts were collected from potatoes prepared for planting, dried, and analysed for α-solanine (S), α-chaconine (CH), and nutrient contents, as well as trypsin inhibitor activity (TIA). The concentrations of S were 10.52 mg and 8.88 mg · g–1 and of CH, 22.67 and 18.70 mg · g–1 of dried sprouts in batch 1 and batch 2, respectively. The proportions of chaconine and solanine in both batches were uniform and amounted to 68% and 32% of the sum. The chemical composition of two batches was similar except for crude fibre content, which was 3.01% and 9.46% in batch 1 and batch 2, respectively. TIA was low and uniform (0.41 mg · g–1)

15

Thermal sterilization affects the content of selected compounds in diets for laboratory animals

63%

Tusnio A. , Taciak M. , Barszcz M. , Paradziej-Łukowicz J. , Oledzka I. , Wiczkowski W. , Szumska M. , Pastuszewska B. , Skomial J.

Journal of Animal and Feed Sciences

|

2014

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tom 23

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nr 4

EN

Autoclaving is the current method of choice for sterilization of diets for specified-pathogen-free (SPF) laboratory animals, but this process may negatively affect the nutrient content (e.g., protein, vitamin) of the diets. Two natural-ingredient diets supplemented with either soyabean meal containing phytoestrogens (S) or with casein (C), and a commercial autoclavable (SN) diet, were autoclaved at 121°C for 20 min (T1) or at 134°C for 10 min (T2). Chemical composition, selected water- and fat-soluble vitamins, and acrylamide were analysed in all non-autoclaved (T0) and autoclaved diets, whereas in S diets, oestrogenic isoflavones were also determined. Autoclaving affected most protein bound to NDF macronutrient contents only to a small degree, except those of NDF and N-NDF. The contents of NDF and N-NDF were increased by T2 and T1; the change was the largest in the SN and the smallest in the C diet. Losses of particular vitamins differed among the diets and treatments. Among the water-soluble vitamins, thiamine, riboflavin and pyridoxine were the least affected, whereas calcium pantothenate was lowered by T1 and T2. Among fat-soluble vitamins, vitamin E was the most stable, while vitamins A and D, the least stable, but even the highest vitamin losses did not exceed 50%. The acrylamide concentration increased more in diets autoclaved at T2 than at T1 and in the S and C diets than in SN. Autoclaving the S diet increased the daidzin and genistin contents and slightly reduced the total isoflavone content. It is concluded that the effects of autoclaving different diets are not uniform, but longer autoclaving at a lower temperature (T1) is less detrimental than shorter treatment at a higher temperature (T2), mainly because of the smaller increase in the NDF and N-NDF contents and acrylamide concentration.

16

Differentially expressed proteins in the blood serum of piglets in response to a diet supplemented with inulin

63%

Herosimczyk A. , Lepczynski A. , Ozgo M. , Skomial J. , Dratwa-Chalupnik A. , Tusnio A. , Taciak M. , Barszcz M.

Polish Journal of Veterinary Sciences

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2015

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tom 18

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nr 3

EN

In the present study we introduced a two-dimensional electrophoresis and matrix-assisted laser desorption/ionisation time of flight mass spectrometry-based proteomic workflow to identify proteins that show altered expression as a result of the addition of 2% of water extract of inulin-type fructans to the diet of growing piglets. This analysis allowed us to detect an average of 240 spots per gel with a mass range from 10 to 250 kDa and a pH ranging from 3 to 10. Twenty protein spots were found to show statistically significant differences in their expression. Of these, 7 protein spots were up-regulated, whereas 13 showed down-regulation in response to the experimental diet. In total, 13 spots were identified, representing 8 distinct gene products. The experimental diet caused a significant change in proteins directly or indirectly involved in hemostasis and the innate immune response. Increased levels of fibrinogen along with decreased plasminogen expression may indicate that a fructan-rich diet favours the deposits of fibrin and promotes blood clotting. We also found increased expression of vitronectin and the alpha subunit of the complement component C8 which may protect the host organism against excessive cytolitic activity of the activated complement. The piglets from the experimental group had slightly increased values of IgG and IgA, whereas the IgM level tended to be decreased. The fructan-rich diet did not have any influence on plasma total cholesterol, HDL and LDL cholesterol and triglyceride levels.

17

Proteome changes in ileal mucosa of young pigs resulting from different levels of native chicory inulin in the diet

63%

Herosimczyk A. , Lepczynski A. , Ozgo M. , Barszcz M. , Marynowska M. , Tusnio A. , Taciak M. , Markulen A. , Skomial J.

Journal of Animal and Feed Sciences

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2018

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tom 27

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nr 3

EN

The analysis of mucosa proteomes was performed using a twodimensional electrophoresis combined with mass spectrometry to determine the effect of dietary level of inulin on protein expression patterns in the ileum. The experiment was carried out on 24 castrated male piglets, allocated to three groups, fed from the day 10 of life an unsupplemented diet (C) or diet supplemented with 1% (T1) or 3% (T2) of native chicory inulin. Samples of ileal tissue and blood were collected after 40 days of feeding. Comparative proteomic analysis of the T1 group showed 10 protein spots with a decreased expression, whereas the T2 diet caused overexpression of 24 spots in comparison to the C diet. Inulin levels differed in their effects on the expression of ileal proteins involved in intracellular molecular mechanisms controlling cell division and growth. The T1 diet down-regulated, whereas the T2 diet induced substantial up-regulation of proteins engaged in transcriptional and translational activities, folding and posttranslational modifications, which may indicate stimulation of epithelial cell proliferation. Inulin did not affect plasma levels of phosphorus, magnesium, calcium and iron in piglets but improved plasma prooxidant-antioxidant balance in animals fed the T2 diet. The results of this study might be considered as preliminary since further confirmation using more sophisticated quantitative proteomic tools is required to better understand and answer the unresolved issues concerning the mechanism underlying the inulin effect on the ileum in growing pigs. Nevertheless, a general insight into how inulin molecules or their fermentation end-products may induce changes in protein expression patterns in the ileum was presented.