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Repair of articular cartilage full thickness defects with cultured chondrocytes placed on polysulphonic membrane - experimental studies in rabbits

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Płończak M. , Czubak J. , Hoser G. , Chwojnowski A. , Kawiak J. , Dudziński K. , Czumińska K.

Biocybernetics and Biomedical Engineering

2008

tom Vol. 28, no. 2

87-93

Autologous osteochondral transplantation is one of the methods. that can be used to create hyaline or hyaline - like repair in the defect area. The purpose of the present study was to repair the full - thickness articular cartilage defects in nine rabbits' knee joints with autologous cultured chondrocytes. An articular cartilage defect was created on the patellar groove of the femur. The defect was filled with the chondrocytes cultured in vitro and placed into the knee on a polysulphonic membrane. 8 weeks after the operation the reparative tissue was analyzed macroscopically and histologically. The surfaces of the reparative tissue were smooth, and the defects were filled with mature hyaline cartilage in five cases. In two cases the reparative hyaline cartilage was immature and there was worse integration of the grafted tissue into the adjacent normal cartilage. The surface of the grafted area was irregular, the reparative tissue was desintegrated and incompletely differentiated. The results suggest that transplantation of the autologous chondrocytes cultured in vitro and placed into the knee on the polysulphonic membrane is effective in repairing of the articular cartilage defect.

Three dimensional polyethersulphone scaffold for chondrocytes cultivation - the future supportive material for articular cartilage regeneration

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Dudziński K. , Chwojnowski A. , Gutowska M. , Płończak M. , Czubak J. , Łukowska E. , Wojciechowski C.

tom Vol. 30, no. 3

65-76

The this paper method of obtaining broad-pore membranes for application as scaffolds for chondrocyte cultivation is described. These membranes were obtained from polyethersulfone. They are characterized by the presence of both macropores of relatively large diameter, as well as micropores. These membranes are intended first of all for use in cultivation of the cartilage cells (chondrocytes). The membranes are obtained by the phase inversion method followed by dissolving cellulose present in the membrane. Cellulose is a macropore precursor. Cellulose is dissolved by means of a copper hydroxide ammonia complex. The membranes obtained are not cyto-toxic. The culture of chondrocytes derived from White New Zeeland breed rabbits developed very well on these membranes. The cell cultures were studied by observation under an optical microscope and scanning electron microscope. The protein mass increase on the membrane was determined by flame analysis. The results of these experiments did not show any negative effects of the membranes on the cell culture. Just the opposite, the cartilage cells development on the membranes proceeded very well. The results obtained show that the membrane developed is a very good scaffold for cell cultivation.