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EN
The effect of vitamin C on the activity of alpha-amylase of plasma and tissuess was examined on 25 rabbits. The activity of alpha-amylase was determined by the method of Caraway and vitamin C by the method of Roe-Kuethner. It was found that sorbic acid increases the activity of alpha-amylase in panreas by 12.5%, in parothidic glands by 5.2%, in liver by 92.3%, in duodenum by 18.7% and in jejunum by 11.1%. The initial decrease of plasma activity of alpha-amylase from 4866 u/l to 4142 u/l coincided with an increase of the content of vitamin C from 14.2 µmol/l to 287.0 µmol/1. After 24 h since the application of vitamin C when plasma level of vitamin C lowered to an initial value the acivity of alpha-amylase increased to 8156 u/l. The application of vitamin C in substantia or food containing high concentration of this vitamin may give some unexpected results. Alterations in alpha-amylase activity by vitamin C may play a valid role when the activity of this enzyme is determined for diagnostic purposes.
RU
У 48 цыплят исследовали поглощение из кишек витамина С в присутствии витамина B₁ и фолиевой кислоты методом in vitro Уильсона и Виземана. Показали, что процесс поглощения аскорбовой кислоты протекает с одинаковой интенсивностью в ближайшей и дальнейшей части тощей кишки. Способность к поглощению витамина С см² площади слепой кишки больше на 22% по сравнению с см² площади тощей кишки. Витамин B₁ существенно уменьшает поглощение аскорбовой кислоты из кишек цыплят, фолиевая же кислота увеличивает этот процесс. Витамин В₁, а также фолиевая кислота, вводимые в корме и промышленных смесях, в веществе как лекарство для цыплят, а также обильно образуемые в пищеварительном тракте цыплят — детерминируют поглощение витамина С из кишек, что следует принимать во внимание в случаях увеличенного запроса организма на аскорбовую кислоту.
EN
The process of absorption of vit. C in the presence of vit. B₁ and folic acid was examined in 48 chickens using Wilson and Wiseman’s method. It was found that the process of ascorbic acid absorption of the same intensity took place at the nearer and futher part of the jejunum. The ability of vit. C absorption by the surface of 1 cm² of the coecum was higher at 22% compared with the same area of the jejunum. Vit. B₁ decreased the process of ascorbic acid absorption from the chickens intestines; in contrast folic acid augmented this process. Vit. B₁ and folic acid given with fodder and industrial mixtures as a drug for poultry, and produced abundantly in the alimentary tract of chickens influence vit. C absorption from intestines; it should be taken into acount in cases of increased needs of organisms for ascorbic acids.
RU
У 45 цыплят-бройлеров исследовали поглощение аскорбовой кислоты (витамина С) методом перфу- зированной петли в опытах in vivo. Показали, что поглощение аскорбовой кислоты из тощей и слепой кишек растет с ее концентрацией в просвете кишки. В низших концентрациях она поглощается по принципу активного транспорта, в высших же по принципу пассивной диффузии. Витамин груп­пы В — хлорид холина уменьшает поглощение ас­корбовой кислоты из кишек цыплят. Поэтому при скармливании цыплятам смесей, богатых хлоридом холина, в условиях, когда эдогенная продукция витамина С недостаточна, дополнительный ввод ас­корбовой кислоты может оказаться очень полезным.
EN
Absorption of ascorbic acid (Vitamin C) was in­vestigated in 45 broiler chickens in vivo by the method of perfused intestinal loop. In chickens absorption of ascorbic acid from jejunum and coecum increased along with an increase of its concentration in the intestine. At lower concentrations ascorbic acid is absorbed actively (mechanism of an active transport is operating), at higher concentrations it is absorbed passively (mechanism of a passive diffusion). Vitamin of group В — choline chloride decreases absorption of ascorbic acid from the intestines of chickens. The­refore when chickens are fed mixtures rich of choline chloride and when endogenic production of vitamin C does not cover its intake, addition of ascorbic acid to a diet is valuable.
EN
The observations were done on 45 broilers at the age of 2—4 months. Using the method of perfused intestinal loop vivo, the absorption of vitamin C from jejunum and coecum at the presence of volatile fatty acids: acetic, propionic and butyric and separately butyric was determined. Ascorbic acid was determined by the method of Roe-Kuethner. It was found that the three basal volatile fatty acids and butyric acid alone decreased absorption of vitamin C and that this decrease depended upon the concentration of volatile fatty acids. Increased concentration of the acids lowered the rate of vitamin C absorption. Volatile fatty acids at a concentration of 50 mM/L lowered absorption of vitamic C in jejunum up to 80,8% (from 2.6 to 2.1 mg/L/cm²/60 min.) and in coecum up to 87.8% (from 4.1 to 3.6 mg/L/cm²/60 min.). Higher concentrations of volatile fatty acids as 130 mM/L lowered absorption of vitamic C more intensively up to 65.4% in jejunum (from 2.6 to 1.7 mg/L/cm²/60 min.) and in coecum up to 68.3% (from 4.1 to 2.8 mg/L/cm²/60 min.). The most active appeared to be propionic acid then butyric and acetic acid. Food of a high content of carbohydrates produces volatile fatty acids in the alimentary tract of poultry decreasing absorption of vitamin C. Therefore enrichment of food with vitamin C or application of vitamin C in poultry is necessary.
EN
The influence of vit. B-compositum of the synthesis of ascorbic acid in chickens was determined. The chickens were divided into three groups: a) group 1 containing 10 animals served as the control, b) group II (10 chickens) was given vit. B-compositum intramuscularly once a day, c) group III (10 chickens) received vit. В orally for two weeks as well. The level of ascorbic acid was determined post mortem using Roe-Kuenther’s method; plasma, the wall of stomach, jejunum, caecum and liver were prepared acc. to Zannoni. An increase of vit. C was 55% in the wall of the stomach, 49% in the caecum, 30% in the jejunum and 25% in the liver. The administration of vit. В per os did not influence ascorbic acid synthesis by the wall of the alimentary tract and liver. One may presume that the addition of vit. В to poultry fodder does not increase vit. C synthesis.
RU
У 11 приплодов поросят из одной селекции, пребывающих в одном свинарнике, разделенных нагруппы, получающую Геггойех или нет, определяли гематологические величины в 1 месяц жизни, т.е. 1 до момента отъема. Показали, что существуют значительные разницы в гематологических величинах :Я между отдельными приплодами в обеих подопыт- 1 пых группах помимо одинаковых условий выращивания. Эти разницы касаются, прежде всего, 1 эритроцитов и лейкоцитов. Наблюдаются также , приплоды поросят, не реагирующих ростом гемо- 1 глобина на препарат железа.
EN
Haematological indices were determined in eleven Jitters coming from a single rearing and maintained in the same shed. One group was given Ferrodex and another served as a control. Significant differences were noted in reference to haematological values depending on the group of piglets. However seme litters diid not react to the ferrous drug either and no increase of haemoglobin was observed.
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