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3 Polish Journal of Microbiology

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Extracellular xylanase production by Fusarium species in solid state fermentation

100%

Arabi M. I. E. , Bakri Y. , Jawhar M.

tom 60

nr 3

Fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. In the present study, xylanase production by 21 Fusarium sp. isolates (8 Fusarium culmorum, 4 Fusarium solani, 6 Fusarium verticillioides and 3 Fusarium equiseti) was evaluated under solid state fermentation (SSF). The fungal isolate Fusarium solani SYRN7 was the best xylanase producer among the tested isolates. The effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton seed cake) and incubation period on xylanase production by F.solani were optimized. High xylanase production (1465.8 U/g) was observed in wheat bran after 96 h of incubation. Optimum pH and temperature for xylanase activity were found to be 5 and 50°C, respectively.

RAPD techniques is a useful tool to distinguish Penicillium species

100%

Bakri Y. , Arabi M. I. E. , Jawhar M.

tom 56

nr 4

Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among 13 soil Pénicillium strains originating from widely dispersed areas. Twenty one of the 34 synthetic random primers were found to identify polymorphism in amplification products. The results show a high level of diversity of RAPD markers among the strains. All the strains could be identified by their characteristic amplification profile, using selected random primers. This suggests that RAPD analysis is a useful and reliable assay for characterizing the species of Pénicillium genus.

Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production

100%

Bakri Y. , Jawhar M. , Arabi M. I. E.

Polish Journal of Microbiology

2008

tom 57

nr 1

The restriction of PCR-amplifïed internal transcribed spacers (ITS) of ribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was observed the resolution between clustering of isolates and their xylanase production level.