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EN
The aim of the study was to determine the influence of Yersinia enterocolitica (Y. enterocolitica) experimental infection on the carrying and shedding states of the microorganism by pregnant sows and on the bacteria occurrence in tissues of the infected animals and aborted or stillborn piglets. Twelve pregnant sows were divided into 4 groups and infected per os on 33 – group I (n=3), 54 – group II (n=3) and 89 – group III (n=3) day of pregnancy with the Y. enterocolitica strain isolated from the palatine tonsil of aborted swine fetus. The control group (n=3) remained uninfected. Rectal, oral and vaginal swabs from sows, placentas and the specimens of tissues from stillborn piglets were collected for bacteriological examination. Eight weeks after delivery, the sows were slaughtered and samples of internal organs were subjected to bacteriological examination. Pregnancy in all groups of sows took a normal course, and no cases of abortion were observed. Y. enterocolitica was isolated from oral, rectal and vaginal swabs of all infected sows. The number of stillborn piglets in the litters was highest in group III, where two macerated fetuses with putrefactive lesions were found. The bacteria were isolated from tissues of stillborn piglets in groups I and III, and only from placenta in group II. In rectal swabs of piglets in all groups, Y. enterocolitica was not isolated. The results of experimental infection of pregnant sows with Y. enterocolitica revealed that in animals infected in the last part of pregnancy, the microorganisms were isolated most frequently from vaginal, rectal and oral swabs of sows as well as from internal organ tissues of stillborn piglets.
EN
The purpose of the studies was to determine the proliferation activity of T and B lymphocytes as well as the metabolic and potential killing activity of blood phagocytes in pigs after the immunomodulation with the Bioimmuno preparation and/or immunisation with the 'Respisure One' vaccine against mycoplasmal pneumonia of swine. The studies were performed on piglets at the age of 4 weeks, divided into four groups of seven animals each. The biopreparations were administered according to the following pattern: Bioimmuno (1 kg/50 kg of feedstuff) for 48 h before vaccination with Respisure One (2 ml/animal i.m.) on the 28th d of life (group I), Bioimmuno (1 kg/50 kg of feedstuff) on days 26 and 27 of life (48 h before vaccination with Respisure One of groups I and III) (group II), Respisure One on the 28th d of life (group III) and PBS (2 ml/animal i.m.) simultaneously with vaccination of groups I and III (group C - control). The proliferation activity of T and B lymphocytes (MTT test) and the metabolic and potential killing activity of blood phagocytes (RBA and PKA tests) were determined in whole blood. Statistically significantly higher (P<0.05) proliferation activity of Con A-stimulated T lymphocytes as well as LPS-stimulated B lymphocytes were found in the stimulated and immunised group I and the immunised group III in comparison with the stimulated group II and group C. Higher metabolic and potential killing activities of the phagocytes were also detected in all experimental groups compared with the control one. The studies have demonstrated that the Bioimmuno immunomodulator and/or the Respisure One vaccine stimulate the proliferative response of T and B lymphocytes, as well as they increase the metabolic and potential killing activity of blood phagocytes, contributing to the improvement in the immune system functioning and to the protection of the swine organism against the infections of the respiratory system.
EN
The purpose of the study was to determine the influence of the Bioimmuno administration in feed and/or immunisation with the 'Respisure' One vaccine against mycoplasmal pneumonia of swine on the development of non-specific immune response expressed by the level of acute phase proteins (APP). The study was performed on 28 piglets at the age of 4 weeks, divided into four equal groups. The biopreparations were administered according to the following pattern: group I - Bioimmuno (1 kg/50 kg of feedstuff) for 48 h and then vaccination with Respisure One (2 ml/animal i.m.), group II - Bioimmuno ( 1 kg/50 kg of feedstuff) for 48 h, group III - Respisure One (2 ml/animal i.m.), and group C (control) - PBS (2 ml/animal i.m.). Serum concentration of APP: C- reactive protein, haptoglobin, and ceruloplasmin were determined on days 0, 3, 7, 14, and 21 after immunomodulation and/or immunisation. The statistically significantly lower (P<0.05) levels of all determined APP in the experimental groups in comparison with the control group were demonstrated. This proves the protective value of the biopreparations applied in specific patterns, through their beneficial influence on the decrease in consequences of the respiratory system infections in pigs.
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