Wyniki wyszukiwania - Biblioteka Nauki

1

Zróżnicowanie genotypowe i analiza stabilności plonowania i parametrów technologicznych nasion fasoli karłowej (Phaseolus vulgaris L.)

100%

Boros L. , Wawer A.

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tom 555

PL

Oceniono efekty genotypowe oraz analizowano stabilność plonowania i parametry technologiczne nasion krajowych odmian fasoli karłowej uprawianej przez sześć sezonów wegetacyjnych w Radzikowie. Stwierdzono istotność różnic genotypowych dla sezonów wegetacyjnych oraz istotność interakcji genotypowo-sezonowych dla wszystkich badanych cech. Analiza komponentów wariancji wykazała, że dla masy 100 nasion oraz zawartości łuski, zawartości popiołu i czasu gotowania - komponent genotypowy znacząco przewyższał komponent genotypowo-sezonowy. Absorpcja wody, elektroprzewodnictwo roztworów to parametry o wysokim udziale obydwu komponentów wariancji. Najwyższy udział komponentów wariancji, innych niż genotypowy, stwierdzono dla plonu nasion z poletka oraz zawartości białka. Analiza stabilności według procedury Francis & Kannenberg pozwoliła na pogrupowanie i wyodrębnienie genotypów łączących pożądany potencjał plonowania z korzystnymi, przynajmniej dwiema, cechami kulinarnej jakości oraz niską zmiennością w latach. Wytypowane odmiany mogą być wykorzystane w pracach hodowlanych w celu poprawy określonych parametrów w procesie hodowlanym.

EN

The study was conducted to estimate genotypic effects and analysis of seed yield stability and technological parameters of the seeds of 21 dry hean cultivars of Polish origin grown for six years at one location. Significant genotype and seasons differences were observed for all parameters. Also all traits showed significant genotype x growing seasons interactions. Variance component estimates showed that genotypic components for 100 seed mass, testa content, ash content and cooking time was substantially larger than that for season and genotype x season components, indicating that genotypie effects predominated over environmental effects for these parameters. The highest values of non-genetic sources of variance were found for seed yield per plot and protein content. Stability analyses with Francis & Kanneberg genotype grouping techniąue allowed to identify genotypes that posses combinations of high yield potential, with at least two favorable parameters of culinary quality with a low year to year variation. The great variability of evaluated attributes suggests that these selected cultivars may be useful as parents in hybridization programs to produce dry bean with improved traits.

2

Zróżnicowanie odmianowe parametrów fizyko-chemicznych i czasu gotowania nasion grochu siewnego (Pisum sativum L.)

100%

Boros L. , Wawer A.

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nr 260-261

3

Ocena podatności odmian grochu siewnego na porażenie grzybem Mycosphaerella pinodes w testach w warunkach kontrolowanych i polowych z kontrolowaną infekcją

100%

Boros L. , Wawer A.

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tom 522

PL

W pracy dokonano oceny genotypowego zróżnicowania poziomu odporności odmian i linii kolekcyjnych grochu siewnego na porażenie grzybem Mycosphaerella pinodes oraz wskazano potencjalne źrodła podwyższonej odporności dla praktycznej hodowli. Badaniami objęto 28 genotypów grochu (16 odmian z Krajowego Rejestru, 4 linii IHAR, 3 odmiany zagraniczne i 5 linii z Pullman o znanej odporności) w celu oceny zakresu zmienności nasilenia zgorzeli siewek oraz podatności/odporności liści i łodyg siewek w warunkach kontrolowanych na porażenie grzybem M. pinodes. Formy te poddano również ocenie w doświadczeniu polowym z kontrolowaną infekcją. Stwierdzono istotność genotypowego zróżnicowania podatności na porażenie M. pinodes w warunkach kontrolowanych i polowych z kontrolowaną infekcją. Wyodrębniono grupę genotypów grochu o niższej podatności na porażenie M. pinodes. Przeanalizowano zależności występujące pomiędzy wynikami ocen w różnych testach. Uwzględnienie wyników oceny podatności będzie pomocne hodowcom w wyborze komponentów do krzyżowań.

EN

The objective of this study was to evaluate genotypic differences in resistance to M. pinodes infections among cultivars and lines of field pea in order to find out potential sources of higher resistance for practical breeding. The assessment of susceptibility to M. pinodes was evaluated on 28 field pea genotypes (16 cultivars from Polish National List, 4 own lines, 3 foreign cultivars - among them cv. Radley and 5 accessions from USDA collection, previously identified as resistant) in two tests under controlled conditions and in the field with controlled infection. Tested genotypes differed significantly in their susceptibility to M. pinodes under these tests. Genotypes of low susceptibility were identified. Analysis of association among the results of different tests was done. Obtained results will be of assistance for breeders in selection of the components to crossing programme.

4

Garden pea varietal susceptibility to Mycosphaerella pinodes and its effect on yield components of single plants

100%

Boros L. , Wawer A.

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tom 70

5

Warta - nowa odmiana fasoli karlowej; wartosc rolnicza oraz parametry technologiczne nasion

80%

Boros L. , Szyrmer J. , Wawer A.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

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2001

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tom 76.1

25-33

6

Response of pea (Pisum sativum L.) cultivars and lines to seed infection by Ascochyta blight fungi

80%

Marcinkowska J. , Boros L. , Wawer A.

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2009

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tom 59

7

Poziom i stabilność plonowania odmian soi o różnej wczesności w zróżnicowanych warunkach agro-klimatycznych

80%

Boros L. , Wawer A. , Borucka K.

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2019

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nr 285

8

Komputerowa symulacja dynamikirozprzestrzeniania się wirusów

80%

Wawer A. , Nielek R. , Kotowski R.

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tom Vol. 5, Nr 1

115-122

PL

W pracy zaprezentowano model rozprzestrzeniania się wirusów łączący w sobie opis systemów wieloagentowych, analizę sieci społecznych i teorie zarażania się. Zbadano możliwość zastosowania prezentowanego podejścia do modelowania epidemii chorób.Typowa sieć społeczna została uzupełniona o socjologiczne mechanizmy komunikacji, systemową teorię komunikacji i o opis przepływu zasobów. Podjęto próbę uwzględnienia realistycznego opisu modelowania czasu społecznego. Sieć społeczna jest aktualizowana w cyklach rekurencyjnych na podstawie nadchodzących komunikatów: tworzą się nowe połączenia, a istniejące połączenia są mody?kowane. Inne znane modele, np. Huang et al. [2004], których autorzy wprowadzają „model małego świata wykorzystującego automaty komórkowe w celu odzwierciedlenia sieci codziennych kontaktów społecznych”, stosują statyczne struktury sieciowe. Symulacje komputerowe zostały wykonane w języku Java z wykorzystaniem Repast Toolkit. Przeprowadzono obliczenia dla kilku mechanizmów zarażania i z wieloma modelami komunikacji.

EN

A dynamic model combining multiagent systems, social network analysis andcontagion theories is presented. The possibilities of its application in epidemiological modelling are investigated. In the model, typical social network based on a directed graph was extended with sociological mechanisms of communicative behavior, systemtheory of communication and resource ?ow. The proposed approach follows realistic social time modelling. The network is updated in recurrent cycles. Network properties are modi?ed by ongoing communication, new links are established and existing ones are updated. In opposition to our approach, other known methods to simulate spreading of SARS virus, like that of Huang et al. [2004], where authors introduce the "smallworld model that makes use of cellular automata with the mirror identities of dailycontact social networks" make use of static network structure. In order to simulate virus spreading the computer software in Java based on Repast Toolkit was developed and used with several contagion mechanisms and multiple communication models.

9

The effect of alpha-synuclein on initiation of inflammatory reaction in the murine brain

71%

Joniec-Maciejak I. , Wawer A. , Sznejder-Pachołek A. , Wojnar E. , Mirowska-Guzel D.

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nr Suppl.1

EN

INTRODUCTION: Parkinson’s disease (PD), one of the most common neurological disorder, is characterized by the loss of dopaminergic neurons in substantia nigra and striatum (ST). The typical reaction of central nervous system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. The data suggests that increased level of α‑synuclein (ASN), a small protein which is the major component of Lewy bodies, can induce microglia activation. Activated microglial cells release proinflammatory and potentially cytotoxic substances like cytokines. Till now, little is known about in vivo effects of exogenous ASN monomers on initiation of neuroinflammation and neurodegeneration. AIM(S): The aim of the present study was to examine the effect of increased ASN monomers concentration on microglia response and expression of pro‑ and anti‑inflammatory cytokines (interleukin 1α (IL‑1α), IL‑10, IL‑12) in the ST. METHOD(S): Male and female C57Bl/10 Tar mice 9 month-old were used in this study. Human recombinant ASN was bilaterally administered into ST (single treatment – 4 μg / structure, 8 μg per brain) and mice were decapitated after 4 or 12 weeks post injection. The changes in the level of inflammatory factors in ST were evaluated using Real-Time PCR and enzyme-linked immunosorbent assay (ELISA). RESULTS: We observed increased level of a microglia marker – ionized calcium-binding adapter molecule 1 (IBA1) protein after ASN injection into ST. We noticed also some differences in the level of one of the most important pro inflammatory cytokines – IL‑1α. CONCLUSIONS: Our study showed that monomers of ASN are strongly involved in the inflammatory reaction in the murine CNS. Further studies are required to reveal the detailed mechanism of the influence of ASN on neuroinflammation in course of Parkinson’s disease. FINANCIAL SUPPORT: This study was supported by Grant No 1M9/PM 2/16 (Medical University of Warsaw). Research subject was implemented with CePT infrastructure financed by the European Union – The Europan Regional Development Fund within the operational programme “Innovative economy for 2007–2013.

10

Jak to się robi na Wyspach

60%

Wawer A.

Koń Polski

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2009

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tom 44

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nr 07

11

The effect of human interleukin-10 on the nitric oxide synthases expression in MPTP- based model of Parkinson's disease

51%

Schwenkgrub J. , Joniec-Maciejak I. , Ciesielska A. , Sznejder A. , Wawer A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.

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nr S

EN

Parkinson’s disease (PD) is a progressive degenerative disorder, which etiology and pathogenesis remains unknown. Post mortem analysis of PD brain and studies on neurotoxic animal models of PD have provided evidence to support the involvement of oxidative stress and neuroinflammatory processes in the pathogenesis of PD. The high level of nitric oxide (NO) is produced by iNOS during the neuroinflammatory process caused by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) treatment. Under pathological condition NO can easily react with superoxide to form peroxynitrite (ONOOˉ), which is a strong oxidant. In the present study was examined the influence of the increased concentration of IL-10 (an anti-inflammatory cytokine) on the NOS expression in mouse model of PD induced by MPTP. One year-old male C57Bl mice were used in this study. An adeno-associated viral vector expressing the gene for human interleukin-10 (hIL-10) was used to transduce striatal cells 4 weeks prior to MPTP intoxication. Mice were sacrificed at the different time intervals: 1, 7 and 21 days after MPTP injection. Immunohistochemical and western blot analyses provide evidence for the protective properties of AAV2-hIL-10 in the MPTP-induced model of PD. There were reduction in the dopaminergic neuron quantity in SNpc and tyrosine hydroxylase protein in the striatum after MPTP injections, whereas in the group additionally treated with AAV2-hIL10 neuroprotection was observed. Treatment with AAV2-hIL-10 suppressed the MPTP-induced increase in iNOS and 3-nitrotyrosine (3-NT) expression in the midbrain.

12

The influence of IL-10 on the inflammatory reaction changes in the mice after 1-methyl-4-phenyl-1,2,3,6-tertahydropyridine (MPTP) treatment

51%

Sznejder A. , Joniec-Maciejak I. , Ciesielska A. , Schwenkgrub J. , Wawer A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.

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nr S

EN

Parkinson’s disease (PD) is one of most frequent neurological disorder characterized by the loss of dopaminergic neurons in substantia nigra and striatum. The typical reaction of central neural system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. Microglia activation stimulates astrocytes response, playing important role in neuroimmune reaction. Microglia cells secrete two types of mediators of the inflammatory process: anti- and pro- inflammatory. In the first stage of Parkinson’s disease, pro-inflammatory cytokines have important meaning. We investigated the effect of an adenoassociated viral vector (AAV2) containing the complementary DNA (cDNA) for human interleukine 10 (hIL-10). The aim of the present study was to examine the evaluation of inflammatory reaction changes following increased concentration of hIL-10 in the murine model of PD induced by MPTP. Male C57BL mice 12 month-old were used in this study. AAV2 vector was bilateraly administered into striatum at 7, 21, 28 days prior to MPTP intoxication. We observed changes in the morphology of microglia cells, infiltration of lymphocytes T (population of CD3+, CD4+ and CD8+) and some differences in the level of one of the most important pro inflammatory cytokines – IL-1α. Our study showed that IL-10 is strongly involved in the inflammatory reaction in the murine model of Parkinson’s disease induced by MPTP. After MPTP intoxication we observed the increase of activated microglia cells, infiltration of lymphocytes T and higher level of IL-1α mRNA. AAV2-hIL-10-treated mice displayed a significant decrease in the activated microglia cells, elevated expression of IL-10 receptors observed on glia cells, strong infiltration of lymphocytes T (mainly CD4+ and CD3+, less CD8+) and minor expression of IL-1α mRNA. Further research must be conducted to provide more evidence of protective role of IL-10 in Parkinson disease.

13

The influence of AAV-mediated gene transfer of human interleukin 10 on the neurodegenerative process in the murine model of parkinson's disease

51%

Wawer A. , Joniec-Maciejak I. , Schwenkgrub J. , Sznejder A. , Ciesielska A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.

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nr S

EN

Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. It is characterized by a progressive loss of dopaminergic neurons, which occurs mainly in the substantia nigra (SN), resulting in the loss of nerve terminals, accompanied by a decreased concentration of dopamine (DA) and its metabolites in the striatum. Concurrently with neurodegeneration, a chronic inflammation occurs in the affected regions of the brain. Mechanisms and etiology of the neurodegeneration are still unknown. One potential strategy for therapy of PD is to reduce the neurodegeneration by inhibiting the inflammatory reaction. Nowadays, there are high hopes for the gene therapy based treatment. This involves using a noninfectious virus administered directly into the brain. In this study a transfer of AAV vector containing the complementary DNA for human interleukin 10 (IL-10) into the nigrostriatal pathway was used. IL-10 is one of the major antiinflammatory cytokines. The aim of the present study was to examine the expression of human IL-10, administered into striatum by viral vector AAV2-hIL-10 and investigate the influence of this cytokine on neurodegenerative process in the murine model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). One year old male C57Bl mice were used in this study. The expression of human IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). To evaluate the influence of the human IL-10 on neurodegeneration concentrations of striatal DA, 3,4-dihydroxyphenyl acetic acid and homovanillic acid were measured by high performance liquid chromatography (HPLC). The findings demonstrate human IL-10 secretion in the mouse brain after striatal infusion of AAV2-hIL-10. This study suggests that human IL-10 delivered by an AAV2 vector preserves nigrostriatal function after MPTP intoxication (lower decrease in DA concentration). IL-10 can play a vital role in inhibition of inflammatory reaction or surviving cells stimulation.