Phytochemical and in vitro antioxidant assessment of Yoyo bitters

• Autorzy: ,,,,,,,,, , Franklyn N. Iheagwam , Mariam I. Sholeye , Itoroobong A. Umoren , Babatunde O. Adetuyi , Adebanke E. Ogundipe , Adefoyeke A. Braimah , Tobi S. Adekunbi , Oluseyi E. Ogunlana , Olubanke O. Ogunlana
• Języki publikacji: EN

Abstrakty

EN

In this paper, herbal bitters are widely used due to their numerous acclaimed health benefits in many Nigerian homes; however, many have not been subjected to scientific scrutiny. The aim of this study was to determine the phytochemical composition and antioxidant capacity of a non-alcoholic polyherbal formulation, Yoyo bitters, towards validating its broad pharmacological claims. The phytochemical components of Yoyo bitters were ascertained by phytochemical screening assays and gas chromatography-mass spectrometry (GC-MS). The antioxidant activity was investigated in vitro using 2,2-diphenyl-1-picryhydrazyl (DPPH) radical, hydrogen peroxide (H2O2) scavenging activity, total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) assays. Qualitative phytochemical analysis of Yoyo bitters showed the presence of saponins, tannins, flavonoids, terpenoids, cardiac glycosides and anthocyanins. The total phenols, flavonoids, flavanols, tannins and carotenoids content were 14.741 ±0.64 mg GAE/ml, 0.152 ±0.01 mg RE/ml, 0.437 ±0.02 mg RE/ml, 0.368 ±0.04 mg TAE/ml and 0.016 ±0.00 mg CAE/ml respectively. GC-MS chromatogram revealed the presence of forty-three (43) phytochemical compounds with D-allose (41.81%), 1,6-anhydro-beta-D-glucofuranose (24.15%), 5-hydroxymethylfurfural (8.02%) and Z-6-pentadecen-1-ol acetate (3.50%) as the most abundant constituents. Yoyo bitters demonstrated effective antioxidant activity against DPPH and H2O2 with IC50 values of 0.492 mg/ml and 0.629 mg/ml respectively compared to ascorbic acid of 0.161 mg/ml and 0.130 mg/ml respectively. Total antioxidant capacity and ferric reducing antioxidant power of Yoyo bitters were 0.432 mg AAE/ml and 2.236 mg AAE/ml respectively. This study validates the antioxidant capacity of Yoyo bitters and provides chemical basis for its acclaimed pharmacological actions.

Słowa kluczowe

EN

GCMS; Yoyo herbal bitters; antioxidant assay; in vitro; phytochemical analysis

• Wydawca: Scientific Publishing House „DARWIN”
• Czasopismo: World News of Natural Sciences
• Rocznik: 2021
• Tom: 37
• Strony: 1-17

Twórcy

autor

,,,,,,,,,

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Franklyn N. Iheagwam

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Mariam I. Sholeye

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Itoroobong A. Umoren

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Babatunde O. Adetuyi

• Department of Natural Sciences, Biochemistry Unit, Precious Cornerstone University, Ibadan, Oyo State, Nigeria

autor

Adebanke E. Ogundipe

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Adefoyeke A. Braimah

• Independent Researcher, 4 Ilupeju Street, Anisere, Sango, Ota, Ogun State, Nigeria

autor

Tobi S. Adekunbi

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

autor

Oluseyi E. Ogunlana

• Department of Biological Sciences, Crawford University, Igbesa, Ogun State, Nigeria

autor

Olubanke O. Ogunlana

• Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria

Bibliografia

• [1] World Health Organisation. National policy on traditional medicine and regulation of herbal medicines: Report of WHO global survey. Geneva: WHO Press; 2005. 5-16p.
• [2] Tilburt CJ, Kaptchuk JT. Herbal medicine research and global health: an ethical analysis. Bulletin of the World Health Organisation 2008; 86: 577-656
• [3] Bandarayanake WM. Quality control, screening, toxicity and regulation of herbal drugs. In: Ahmad I, Aqil F, Owais M (Eds.). Modern phytomedicine: turning medicinal plants into drugs. Weinheim: WILEY-VCH Verlag GmbH & Co, KGaA; 2006. 25-57 p.
• [4] Hoffmann D. Healthy digestion: a natural approach to relieving indigestion, gas, heartburn, constipation, colitis and more. (1st Ed) Hoffmann, D. (Ed.). Massachussetts: Storey Publishing LLC; 2000. 1-124 p. ISBN 1580172504
• [5] Ukoha OP, Nnamdi OL, Cemaluk CE, Madus PE. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry 2011; 5: 237-244
• [6] Rani V, Deep G, Singh RK, Palle K, Yadav UC. Oxidative stress and metabolic disorders: pathogenesis and therapeutic strategies. Life Sciences 2016; 148: 183-193
• [7] Harborne JB. Phytochemical methods. In: Harbone, J.B. (Ed.). A guide to modern techniques of plant analysis. London, England: Chapman and Hall Ltd; 1973. 49-188 p. ISBN 9789782462190
• [8] Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd.; 1993. 191-289 p. ISBN 9782462195.
• [9] Sharma A, Goyal R, Sharma L. Potential biological efficacy of Pinus plant species against oxidative, inflammatory and microbial disorders. BMC Complementary and Alternative Medicine 2015; 16(1): 35
• [10] Hossain MA, Wakabayashi H, Goda F, Kobayashi S. Effect of immunosuppressant FK506 and d-allose on allogenic orthotopic liver transplantation in rats. Transplant Proceedings 2000; 32: 2021-2023
• [11] Sui L, Dong Y, Watanabe Y, Hatano N, Tsukamoto I, Izumori K, Tokuda M. The inhibitory effect and possible mechanisms of d-allose on cancer cell proliferation. International Journal of Oncology 2005; 27: 907-912
• [12] Murata A, Sekiya K, Watanabe Y, Yamaguchi F. A novel inhibitory effect of d-allose on production of reactive oxygen species from neutrophils. Journal of Bioscience and Bioengineering 2003; 96: 89-91
• [13] Zhao L, Chen J, Su J, Li L, Hu S, Li B, Zhang X, Xu Z, Chen T. In vitro antioxidant and antiproliferative activities of 5-hydroxymethylfurfural. Journal of Agricultural and Food Chemistry 2013; 61(44): 10604-10611
• [14] Sirigiri CK, Kandru A. GC-MS analysis of biologically active compounds in Canthium parviflorum lam. leaf and callus extracts. International Journal of ChemTech Research 2017; 10(6): 1039-1058
• [15] Saikarthik J, Saraswathi I, Vijayakumar J, Vijayaraghavan R. Phytochemical analysis of methanolic extract of seeds of Mucuna pruriens by gas chromatography mass spectrometry. International Journal of Pharmaceutical Sciences and Research 2017; 8(7): 2916-2921
• [16] Parthipan MV, Mohan DN, Sivakama S, Karuppusamy S, Mohan VR. GC-MS analysis of leaf and stem bark of Cleidion nitidum. Asian Journal of Pharmaceutical and Clinical Research 2014; 7(2): 1-7
• [17] Rahman MM, Ahmad SH, Mohamed MTM, Ab Rahman AZ. (2014). Antimicrobial compounds from leaf extracts of Jatropha curcas, Psidium guajava and Andrographis paniculate. The Scientific World Journal 2014; 2014: 1-8
• [18] Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. African Journal of Biochemistry Research 2010; 4(7): 191-195.
• [19] Takahashi K, Satoh H, Kakuchi T, Miura M, Sasaki A, Sasaki M, Kaga H. Formation kinetics of levoglucosan from glucose in high temperature water. Chemical Engineering Journal 2009; 153: 1-3
• [20] Wei Y, Chunhua Q. Synthesis of epigoitrin from R-(+)-4-hydroxy-γ-butyrolactone. Journal of Heterocyclic Chemistry 2013; 50(6): 1290-1293
• [21] Onyeaghala AA, Omotosho IO, Shivashankara, AR. Chemical isolation and characterization of a popular detoxifying herbal remedy Yoyo bitters using GC-MS, NMR and FTIR analysis. International Research Journal of Pure and Applied Chemistry 2015; 6(4): 190-200
• [22] Kokate CK. Practical pharmacognosy. (3rd ed.). Kokate, C.K. (Ed.). New Delhi, India: Vallabh Prakashan; 1994. 112-120 p.
• [23] Anionye JC, Onyeneke EC. Composition and in vitro antioxidant capacity of yoyo bitters. European Journal of Biological Sciences 2016; 8(3): 108-115
• [24] Sodipo OA, Akiniyi JA, Ogunbamosu JU. Studies on certain characteristics of extracts of bark of Pansinystalia macroceras pierre exbeille. Global Journal of Pure and Applied Sciences 2000; 6: 83-87
• [25] Atmani D, Nassima C, Dina A, Meriem B, Nadjet D, Hania B. Flavonoids in human health: from structure to biological activity. Current Nutrition and Food Science 2009; 5: 225-237
• [26] Haslam E. Natural polyphenols (vegetable tannins) as drugs: possible modes of action. Journal of Natural Products 1996; 59: 205-215
• [27] Traore F, Faure R, Ollivier E, Gasquet M, Azas N, Debrauwer L, Keita A, Timon-David P, Balansard G. Structure and antiprotozoal activity of triterpenoid saponins from Glinus oppositifolius. Planta Medica 2000; 66: 368-371
• [28] Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. Journal of Pharmacognosy and Phytochemistry 2013; 1(6): 168-182
• [29] Prassas I, Diamandis EP. Novel therapeutic applications of cardiac glycosides. Nature Reviews Drug Discovery 2008; 7: 926-935
• [30] Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 2005; 4(7): 685-688
• [31] Kim SY, Kim JH, Kim SK, Oh MJ, Jung MJ. Antioxidant activities of selected oriental herb. Journal of the American Oil Chemists' Society 1994; 71: 633-640
• [32] Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry 2005; 53(10): 4290-4302
• [33] Duan XJ, Zhang WW, Li WXM, Wang BG. Evaluation of antioxidant property of extract and fraction obtained from red alga Polysiphonie urcelata. Food Chemistry 2006; 95: 1022-1037

• Typ dokumentu: article