Disturbances of cow oocyte maturation by phytoestrogens

• Autorzy: Borzym E. , Bobowiec R. , Kosior-Korzecka U. , Martelli F. , Burmanczuk A.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to elucidate the influence of different phytoestrogens (PEs) such as izoflavons (represented by genistein), stilbens (represented by resveratrol (RES)), lignans (represented by enterodiol) and coumestans (represented by coumstrol) on the maturation process of isolated bovine oocytes. We assumed that their influence on the rate of oocyte growth and maturation would be related primarily to their estrogenic activity (isoflavones and coumestans are similar to the 17β estradiol - E₂, the most potent oestrogen). Eighty to one hundred oocytes were cultured in 4 ml of the medium in six-well multidishes. Cumulus oocyte complexes (COCs) were matured at 39°C in humidified air containing 5% CO₂ for 24 hours. Four phytoestrogens were tested at two concentrations: 10 µg/ml and 100 µg/ml. These results clearly show that there is no significant relation between PEs and the survival of cow oocytes. However, under the influence of different PEs the expansion of oocytes was suppressed, especially when genistein and coumestrol were introduced into the culture medium. It was noted that the addition of the phytoestrogens into the medium decreased the rate of oocyte maturation. The highest increase of immature oocytes (p ≤ 0.05) was noted after the addition of coumestrol (30.77%) and genistein (20%) (fig. 4). The most pronounced suppression of oocyte maturation by genistein and coumestrol observed in our research indicates, therefore, that there is a direct relationship between the potency of the estrogenic activity of particular PEs and the inhibition of oocyte maturation. We concluded from our studies that PEs may disturb the conception and fertilisation rate in the cow by prolonging cow oocyte maturation.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 09
• Opis fizyczny: p.1107-1111,fig.,ref.

Twórcy

autor

Borzym E.

• Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland

autor

Bobowiec R.

autor

Kosior-Korzecka U.

autor

Martelli F.

autor

Burmanczuk A.

Bibliografia

• 1. Arlotto T., Schwartz J. L., First N. I., Leibfried-Rutledge M. L.: Aspect of follicle and oocyte stage that affect in vitro maturation and development of bovine oocytes. Theriogenology 1996, 45, 943-956.
• 2. Fałkowska-Podstawka M., Bobowiec R., Rzeski W.: The effect of interferon-τ and ovarian steroids on the proliferation of bovine endometrial cells in vitro. Pol. J. Vet. Sci. 2006, 4, 239-246.
• 3. Gilchrist R. B., Thompson J. G.: Oocyte maturation: Emerging concept and technologies to improve developmental potential in vitro. Theriogenology 2007, 67, 6-15.
• 4. Henry L. A., Witt D. M.: Resveratrol: phytoestrogen effects on reproductive physiology and behavior in female rats. Hormones and Behavior 2002, 41, 220-228.
• 5. Jung T., Fulka J. Jr., Lee C., Moor R. M.: Effects of the protein phosphorylation inhibitor genistein on maturation of pig oocytes in vitro. J. Reprod. Fertil. 1993, 98, 529-535.
• 6. Kątska L., Smorąg Z.: The influence of culture temperature on in vitro maturation of bovine oocytes. Anim. Repr. Sci. 1985, 9, 205-212.
• 7. Khodabandehlou H., Hoffmann B., Pallauf J.: Investigations in central Hessia on the occurrence of estrogenic activity content of cattle feed. Dtsch Tierärztl. Wochenschr. 1997 Aug 104:8 291-4
• 8. Krischek C., Meinecke B.: In vitro maturation of bovine oocytes requires polyadentylation of mRNAs coding proteins for chromatin condensation, spindle assembly, MPF and MAP kinase activation. Anim. Reprod. Sci. 2002, 73, 129-140.
• 9. Knight D. C., Eden J. A.: Phytoestrogens - a short review. Maturitas 1995, 22, 167-175.
• 10. Nagai T.: The improvement of in vitro maturation systems for bovine and porcine oocytes. Theriogenology 2001, 55, 1291-1301.
• 11. Nogowski L.: Effects of phytoestrogen-coumestrol on lipid and carbohydrate metabolism in young ovariectomized rats may be independent of its estrogenicity. J. Nutr. Biochem. 1999, 10, 664-669.
• 12. Nowicka E., Szkudelski R., Nogowski L.: Działanie fitoestrogenów na organizm człowieka i zwierząt. Medycyna Wet. 2006, 62, 736-738.
• 13. Pelissero C., Lenczowski M. J. P., Chinzi D., Davail-Cuisset B., Sumpter J. P., Fostier A.: Effects of flavonoids on aromatase activity, an in vitro study. J. Steroid Biochem. Melec. Biol. 1996, 57, 215-223.
• 14. Pujol M., Lopez-Bejar M., Paramio M. T.: Developmental competence of heifer oocytes selected using the brilliant cresyl blue (BCB) test. Theriogenology 2004, 61, 735-744.
• 15. Tatemoto H., Terada T.: Involvement of cumulus cells stimulated by FSH in chromatin condensation and the activation of maturation - promoting factor in bovine oocytes. Theriogenology 1998, 49, 1007-1020.
• 16. Thompson J. G.: The impact of nutrition of the cumulus oocyte complex and embryo on subsequent development in ruminants. J. Reprod. Dev. 2006, 52, 169-175.
• 17. Van Cauwenberge A., Alexandre H.: Effect of genistein alone and in combination with okadaic acid on the cell cycle resumption of mouse oocytes. Int. J. Dev. Biol. 2000, 44, 409-420.
• 18. Wehrend A., Meinecke B.: Kinetic of meiotic progression, M-phase promoting factor (MPF) and mitogen-activated protein kinase (MAP kinase) activities during in vitro maturation of porcine and bovine oocytes: species specific differences in the length of the meiotic stages. Anim. Reprod. Sci. 2001, 66, 175-184.
• 19. Whitten P. L., Patisaul H. B.: Cross-species and interassay comparisons of phytoestrogen action. Eviron. Health Perspect. 2001, 109, 5-20.
• 20. Woclawek-Potocka I., Okuda K., Acosta T. J., Korzekwa A., Pilawski W., Skarzynski D. J.: Phytoestrogen metabolites are much more active than phytoestrogens themselves in increasing prostaglandin F₂α synthesis via prostaglandin F₂α synthase-like 2 stimulation in bovine endometrium. Prostaglandins & other Lipid Mediators. 2005, 78, 202-217.
• 21. Wójcik-Gładysz A., Romanowicz K., Misztal T., Polkowska J., Barcikowski B.: Effect off intracerebroventricular infusion of genistein on the secretory activity of the GnRH/LH axis in ovariectomized ewes. Anim. Reprod. Sci. 2005, 86, 221-235.
• 22. Yamashita Y., Kawada S., Nakano H.: Induction of mammalian topoisomerase II dependent DNA cleavage by nonintercalative flavonoids, genistein and orobol. Biochem. Pharmacol. 1990, 39, 737-744.

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