Molecular identification of new varieties of Nierembergia linariaefolia [Graham], a native Argentinean ornamental plant

• Autorzy: Escandon A S , Zelener N , La Torre M P , Soto S
• Języki publikacji: EN

Abstrakty

EN

Six Nierembergia linariaefolia clones were selected for ornamental traits during a native germplasm development program. For fingerprinting diagnosis, 13 anchored inter-simple sequence repeat (ISSR) primers and 6 amplified fragment length polymorphism (AFLP) primer-enzyme combinations were used. Both markers revealed high levels of polymorphism, enabling genetic discrimination of the accessions analyzed by using 443 informative ISSRs and 541 AFLP markers. Both molecular techniques are suitable for monitoring genetic diversity in Nierembergia linariaefolia and, under our experimental conditions, they showed correlation coefficients of 0.629 for similarity matrices and of 0.649 in the cophenetic matrices. These results suggest that ISSRs are a good choice for DNA analysis in N. linariaefolia when simple manipulation and a low budget are required.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2007
• Tom: 48
• Numer: 2
• Strony: 115-123
• Opis fizyczny: p.115-123,fig.,ref.

Twórcy

autor

Escandon A S

• Instituto de Floricultura, INTA-Castelar, Argentina

autor

Zelener N

• Instituto de Recursos Biológicos, INTA-Castelar, Argentina

autor

La Torre M P

• Instituto de Floricultura, INTA-Castelar, Argentina

autor

Soto S

• Instituto de Floricultura, INTA-Castelar, Argentina

Bibliografia

• Blair MW, Panaud O, Mc Couch SR, 1999. Inter-simple sequence repeat amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor Appl Genet: 98: 780-792.
• Briard M, Le Clerc V, Mausset AE, Veret A, 2000. A comparative study on the use of ISSR, microsatellites and RAPD markers for varietal identification of carrot genotypes. Acta Hortic: 546: 377-385.
• Cocucci AA, Hunziker AT, 1995. Nierembergia, En: Hunziker AT, ed. Flora Fanerogámica Argentina: 3-14.
• Debener T, 2002. Molecular markers as a tool for analysis of genetic relatedness and selection in ornamentals. In: Breeding for ornamental: Classical and molecular approaches (Vainstein A, ed.). Kluwer Academic Publisher: 329-345.
• De Riek J, 2001. Are molecular markers strengthening plant variety registration and protection? In: Van Huylenbroeck J, Van Bockstaele E, Debergh P, eds. Proc. XX EUCARPIA Symp. Strategies for New Ornamentals. Part I. Acta Hortic 552: 215-223.
• Dendauw J, De Riek JD, Loose M, Van Bockstaele E, 2002. Identification of 33 Chinese Rhododendron species using MATK sequences and AFLP data. In: Van Huylenbroeck J, Van Bockstaele E, Debergh P, eds. Proc. XX EUCARPIA Symp. Strategies for New Ornamentals. Part II. Acta Hortic 572: 169-177.
• Escandón AS, Pérez de la Torre M, Acevedo A, Marcucci-Poltri S, Miyajima I, 2005a. Anchored ISSR as molecular marker to characterize accessions of Jacaranda mimosifolia L. Don. In: Tombolato AFC, Dias-Tagliacozzo GM, eds. Proc. 5th Int. Symp. on New Flor. Crops. Acta Hortic 683: 121-127.
• Escandón AS, Pérez de la Torre M, Soto M, Zelener N, 2005b. Identificación de clones selectos de Nierembergia linariaefolia mediante microsatélites anclados. Rev Invest Agropecu 34: 3-15.
• Fernández ME, Figueras AM, Benito C, 2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet 104: 845-851.
• Gupta PK, Varshney RK, 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113: 163-185.
• Han TH, Van Eck HJ, Dejeu MJ, Jacobsen E, 1999. Optimization of AFLP fingerprinting of organism with a large-sized genome: a study in Alstroemeria spp. Theor Appl Genet 98: 465-471.
• Hoisington D, Khairallah M, González de León D, 1994. Laboratory Protocols: CIMMYT. Applied Molecular Genetics Laboratory, 2nd edn. Mexico, D.F: 2-5.
• Jaccard P, 1908. Nouvelles recherches sur la distribution florale. Bull Soc Vaudoise Sci Nat 44: 223-270.
• Jain A, Apparanda C, Bhalla P, 1999. Evaluation of genetic diversity and genome fingerprinting of Pandorea (Bignoniaceae) by RAPD and inter-SSR PCR. Genome 42: 714-719.
• Jeung JU, Hwang HG, Moon HP, Jena KK, 2005. Fingerprinting temperate japonica and tropical indica genotypes by comparative analysis of DNA markers. Euphytica 146: 239-251.
• Khan JD, Procunier DG, Humphreys Tranquilli G, Schlatter AR, Marcucci-Poltri S, Joppa LR, et al. 2000. Development of PCR based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Sci 40: 518-5249.
• Lapointe FJ, Legendre P, 1992. Statistical significance of the matrix correlation coefficient for comparing independent phylogenetic trees. Syst Biol 41: 378-384.
• Loh JP, Kiew R, Keet A, Gan LH, Gan YY, 1999. Amplified fragment length polymorphism (AFLP) provides molecular markers for the identification of Caladium bicolor cultivars. Annals of Botany 84: 155-161.
• Lombard V, Baril CP, Dubreuil P, Blouert F, Zhang D, 1999. Potential use of AFLP markers for the distinction of rapeseed cultivars. In: Proceedings of the 10th International Rapeseed Congress (Canberra, Australia).
• Manifesto MM, Schlatter AR, Hopp HE, Suárez EY, Dubcovsky J, 2001. Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Sci 41: 682-690.
• Marcucci Poltri SN, Zelener N, Rodríguez Traverso J, Gelid P, Hopp HE, 2003. Selection of a seed orchard of Eucalyptus dunnii based on genetic diversity criteria calculated using molecular markers. Tree Physiology 23: 625-632.
• McGregor CE, Lambert CA, Greyling MM, Louw JH, Warnich L, 2000. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135-144.
• Nybon H, 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13: 1143-1155.
• Paris HS, Yonash N, Portnoy N, Mozes-Daube N, Tzuri G, Katzir N, 2003. Assessment of genetic relationships in Cucurbita pepo (Cucurbitaceae) using DNA markers. Theor Appl Genet 106: 971-978.
• Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P, Taramino G, Motto M, 1998. Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theor Appl Genet 97: 1248-1255.
• Pérez de la Torre M, Acevedo A, Serpa JC, Miyajima I, Escandón AS, 2003. Puesta a punto de la técnica de microsatélites anclados para la caracterización de individuos selectos de Jacarandá. In: Mascarini L, Vilella F, Wright E, eds. Floricultura en la Argentina. Investigación y Tecnología de producción: 3-12.
• Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A, 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breeding 2: 225-238.
• Prevost A, Wilkinson MJ, 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98: 107-112.
• Rongwen J, Acalla MS, Bhagwat AA, Lavi U, Creagan PB, 1995. The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90: 43-48.
• Schut JW, QI X, Stam P, 1997. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Theor Appl Genet 95: 1161-1168.
• Sica M, Gamba G, Montieri S, Gaudio L, Aceto S, 2005. ISSR markers show differentiation among Italian populations of Asparagus acutifolius L. BMC Genetics 6: 17. http://www.biomedcentral.eom/1471-2156/6/17
• Soto MS, Bullrich L, Facciuto G, Hagiwara JC, Mata D, Kobayashi N, et al. 2005. New clones of Nierembergia linariaefolia bred from populations. In: Tombolato AFC, Dias-Tagliacozzo GM, eds. Proc. 5th Int. Symp. on New Flor. Crops. Acta Hortic 683: 407-410.
• Vos P, Hogers R, Bleker M, Reijans M, Van der Lee T, Homes M, et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.
• Wolff K, Zietkiewicz E, Hofstra H, 1995. Identification of Chrysanthemum cultivars and stability of DNA fingerprinting patterns. Theor Appl Genet 91: 439-447.
• Zelener N, Marcucci Poltri SN, Bartoloni N, López CR, Hopp HE, 2005. Selection strategy for a seedling seed orchard design based on both trait selection index and genomic analysis by molecular markers: a case study for Eucalyptus dunnii. Tree Physiology 25: 625-632.
• Zhang D, Germain E, Reynders-Aloisi S, Gandelin MH, 2000a. Development of amplified fragment length polymorphism markers for variety identification in rose. Acta Hortic 508: 113-120.
• Zhang D, Dirr MA, Price RA, 2000b. Discrimination and genetic diversity of Cephalotaxus accessions using AFLP markers. J Am Soc Hortic Sci 125: 404-412.
• Zietkiewicz E, Rafalski A, Labuda D, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymorphism chain reaction amplification. Genomics 20: 176-183.