Laccase production by Trameteshirsuta, characterization, and its capability of decoloring chlorophyll

• Autorzy: Liu J. , Liu W. , Cai Y. , Liao X. , Huang Q. , Liang X.
• Języki publikacji: EN

Abstrakty

EN

The present study focused on laccase production, characterization, and its involvement in chlorophyll decolorization. Extracellularlaccase, with the highest activity of 11 U/ml on day 8, was efficiently produced from Trametes hirsuta in 5 l bioreactor with optimized media comprising dual carbon sources, glucose and water hyacinth. A laccase was then purified from the supernatant to homogeneity with purification fold of 9.51 and recovery of 39.8% and an estimated molecular mass of 62 kDa by SDS-PAGE. The laccase showed activity at pH 2–6 and temperature 30–80°C and was relatively thermally stable at below 70°C and neutral pH. The laccase was applied to decolorize chlorophyll under different factors: temperature, pH, mediator, metal ions, and enzyme dosage. Other fungal laccases were also found to be able to degrade chlorophyll with rating from 52% to 88% following 1 h treatment with two laccase dosages (5 or 10 U/ml) in the absence of any other mediators at room temperature. These findings may be an important step in developing new, important, and commercially viable industrial applications for laccase enzymes.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2014
• Tom: 63
• Numer: 3
• Opis fizyczny: p.323-333,fig.,ref.

Twórcy

autor

Liu J.

• Fermentation Technology Division, School of Bioengineering, Huanghuai University, Zhumadian 463000, China
• The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

autor

Liu W.

• The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

autor

Cai Y.

• The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

autor

Liao X.

• The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

autor

Huang Q.

• Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia 30223, United States

autor

Liang X.

• Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, China

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