The effect of 4-aminopyrazolo (3,4-d) pyramidine (4-APP) on some parameters of carbohydrate metabolism in the rat

• Autorzy: Torlinska T. , Malendowicz L.K. , Paluszak J. , Kozlik J. , Nowak M. , Nowak K.W. , Godlewski J.
• Języki publikacji: EN

Abstrakty

EN

Torlińska, T., Malendowicz, L. K, Paluszak, J., Koźlik, J., Nowak, M., Nowak, K. W. and Godlewski, J.: The effect of 4-aminopyrazolo (3,4-d) pyrimidine (4-APP) on some parameters of carbohydrate metabolism in the rat. Acta physiol, pol., 1989, 40 (5-6): 530-534. The study was designed to evaluate some parameters of carbohydrate metabolism in the rat as influenced by 4-APP, an adenine analogue. Adult female rats were injected with 1 mg 4-APP /100 g body weight/ day for 3 days. 4-APP evoked a marked enlargement of the liver with lipid droplets accumulation in hepatocytes. This was accompanied by a marked lowering of the liver glycogen content. Within 3 days 4-APP did not change serum glucose, insulin and free fatty acid concentration. Serum glycogenolytic activity studied in an in vitro system showed 7 times as high glucose releasing ability in 4-APP treated rats as that of the serum of control animals. 4-APP resulted also in a marked enlargement of the adrenal medulla and lowered adrenaline and noradrenaline concentrations in the gland. The possibility of an activation of glycogenolysis in the liver of 4-APP treated rats has been discussed.

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1989
• Tom: 40
• Numer: 5-6
• Opis fizyczny: p.530-534,ref.

Twórcy

autor

Torlinska T.

• Department of Physiology, Department of Histology and Embryology, School of Medicine, Poznan, Poland

autor

Malendowicz L.K.

• Department of Physiology, Department of Histology and Embryology, School of Medicine, Poznan, Poland

autor

Paluszak J.

• Department of Physiology, Department of Histology and Embryology, School of Medicine, Poznan, Poland

autor

Kozlik J.

• Department of Physiology, Department of Histology and Embryology, School of Medicine, Poznan, Poland

autor

Nowak M.

• Department of Physiology, Department of Histology and Embryology, School of Medicine, Poznan, Poland

autor

Nowak K.W.

• Department of Animal Physiology and Biochemistry, School of Agriculture, Poznan, Poland

autor

Godlewski J.

• Department of Pharmacology, School of Medicine, Poznan, Poland

Bibliografia

• 1. Andersen, J. M., Dietschy, J. M. (1976) Regulation of sterol synthesis in adrenal gland of the rat by both high and low density human plasma lipoproteins. Biochem. Biophys. Res. Commun. 72. 880-885.
• 2. Andersen, J. M., Dietschy, J. M. (1976) Cholesterogenesis: Derepression in extrahepatic tissues with 4-aminopyrazolo (3,4-d) pyrimidine. Science 192. 903-905.
• 3. Andersen, J. M., Dietschy, J. M. (1978) Relative importance of high and low density lipoproteins in the regulation of cholesterol synthesis in the adrenal gland, ovary and testes of the rat. J. Biol. Chem. 253. 9024-9032.
• 4. Chang, С. C. (1964) A sensitive method for spectrophotofluorometric assay of cate cholamines. Int. J. Neuropharmacol. 3. 643-649.
• 5. Dole, V. P., Meinertz, H. (1960) Microdetermination of long-chain fatty acids in plasma and tissues. J. Biol. Chem. 235. 2595-2599.
• 6. Good, C., Kramer, H., Somogyi, M. (1933) The determination of glycogen. J. Biol. Chem. 100. 485-491.
• 7. Hems, D. A., Whitton, P. D., Ma, G. Y. (1975) Metabolic actions of vasopressin, glucagon and adrenaline in the intact rat. Biochim. Biophys. Acta 411.155-164.
• 8. Hems, D. A., Whitton, P. D., Rodrigues, L. M. (1978) Rapid stimulation by vasopressin, oxytocin and angiotensin II of glycogen degradation in hepatocyte suspensions. Biochem. J. 172. 311-317.
• 9. Hems, D. A., Whitton, P. D. (1980) Control of hepatic glycogenolysis. Physiol. Rev. 60.1-50.
• 10. Hers, H. G. (1976) The control of glycogen metabolism in the liver. Ann. Rev. Biochem. 45. 167-189.
• 11. Hultman, E. (1959) Rapid specific method for determination of aldosaccharides in body fluids. Nature (London) 103. 183-187.
• 12. Lowry, О. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193. 265-275.
• 13. Malendowicz, L. K., Nowak, M., Paluszak, J., Torlińska, T. (1987) Adrenocortical function in 4-APP treated rats: a coupled stereological and biochemical study. J. Steroid. Biochem. 26. 487-492.
• 14. Nowak, M., Majchrzak, M., Malendowicz, L. K. (1987) Stereologic studies of adrenal cortex of 4-APP-induced lipoprotein-deficient rats. Exp. Pathol. 31. 169-173.
• 15. Paluszak, J., Torlińska, T., Witmanowski, H., Torliński, L. (1984) In vitro serum glycogenolytic activity in rats during hypothermia induced with 2-deoxy-D-glucose. Acta physiol, pol. 35. 237-241.
• 16. Saitoh, Y., Ui, M. (1975) Activation and inactivation of phosphorylase and glycogen synthetase during perfusion of rat liver as influenced by epinephrine, glucagon and hydrocortisone. Biochim. Biophys. Acta. 404. 7-17.
• 17. Torlińska, T., Paluszak, J., (1984) Badania aktywności surowicy krwi szczura in vitro. Folia Med. Cracov. 3-4. 275-286.
• 18. Weibel, E. R. (1979) Stereologic Methods. Vol. 1. Practical methods for biological morphometry. London: Academic Press. 1-415.
• 19. Witters, L. A., Avruch, J. (1978) Insulin regulation of hepatic glycogen synthetase and phosphorylase. Biochemistry 17. 406-410.