Immunoreactivity to galanin in the small intestine of wild boar

• Autorzy: Czujkowska A. , Arciszewski M. B.
• Języki publikacji: EN

Abstrakty

EN

Galanin is a 29/30-amino acid peptide with a wide array of biological activities. At a central level, galanin mediates feeding behavior, nociception, learning and co-transmission. The presence of galanin has been found in the enteric nervous system (ENS) of the stomach, small intestine and large intestine. Although in the ENS of humans, domestic animals and laboratory animals, the presence of galanin-ergic neurons and their chemical coding have been studied, the occurrence and biochemical profile of galanin-ergic neurons in ENS of wildlife animals is still obscure. The aim of the present study was using immunohistochemical methods to evaluate whether and to what extend galanin is expressed in enteric neurons and nerve fibers supplying distinct regions of the wild boar small intestine (duodenum, jejunum, ileum). Double immunohistochemical stainings were applied in order to study the co-localization of galanin with vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and nitric oxide synthase (NOS) in enteric neurons and nerve fibers innervating the wild boar small intestine. Depending on the region of the wild boar small intestine, the expression of galanin was found in 5-10% of myenteric neurons and in 45-65% of submucous neurons. In all three segments of the small intestine, moderately numerous/numerous galanin-immunoreactive (IR) nerve fibers were found between neurons of myenteric and submucous ganglia, smooth muscle circular layer and between gland and villi of the mucosa. In the longitudinal smooth muscle layer of the jejunum and ileum (but not duodenum) the presence of single galanin-IR nerve fibers were found. The presence of single galanin-expressing nerve fibers was localized around duodenal Brunner’s glands, blood vessels of the jejunal submucous layer, as well as in lamina muscularis mucosae of all regions of wild boars small intestines. In all regions of the small intestine, quite numerous galanin-IR submucous neurons (but not myenteric) as well as the smooth muscles-, submucosaand mucosa-supplying galanin-IR nerve fibers co-expressed VIP. In galanin-IR nervous structures of the wild boar duodenum, jejunum and ileum no co-localizations with NOS and NPY have been found.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 01
• Opis fizyczny: p.29-35,fig.,ref.

Twórcy

autor

Czujkowska A.

• Municipal Zoological Garden in Warsaw, Ratuszowa 1/3, 03-461 Warsaw, Poland

autor

Arciszewski M. B.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland

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