Occurrence of F-specific RNA coliphages and microbial indicators in municipal lake water

• Autorzy: Sliwa-Dominiak J. , Tokarz-Deptula B. , Deptula W.
• Języki publikacji: EN

Abstrakty

EN

Recent studies have shown the increasing interest of F-specific RNA bacteriophages (FRNAPH) genotyping to identify major sources of faecal contamination in waters. The aim of the study was to evaluate the presence and relevance of FRNAPH genotyping in lake water. A comparison between the levels of all FRNAPH and each genogroup and that of the standard indicators of faecal pollution (total coliforms – TC and faecal (thermotolerant) coliforms – FC) was performed. Two faecal indicators were detected in lake water: total coliforms (TC) and faecal coliforms (FC). The standardized method was used to quantify these indicators and bacterial concentrations were expressed in the most probable number per 100 ml of water (MPN/10 ml). FRNAPH were determined by the single agar layer (SAL) method using the host strain Escherichia coli Famp (ATCC 700891) in accordance with U.S. EPA Methods 1602. To distinguished between RNA and DNA phages, each FRNAPH isolate was subjected to RNAse sensitivity. All FRNAPH isolates were subjected to the four SYBR Green-based real time PCRs to determine genogroups.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2014
• Tom: 23
• Numer: 2
• Opis fizyczny: p.467-473,fig.,ref.

Twórcy

autor

Sliwa-Dominiak J.

• Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland

autor

Tokarz-Deptula B.

• Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland

autor

Deptula W.

• Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland

Bibliografia

• 1. LEE J.E., LIM M.Y., KIM S.Y., LEE S., LEE H., OH H.-M., HUR H.-G., KO GP. Molecular characterization of bacteriophages for microbial source tracking in Korea. Appl. Environ. Microbiol. 75, 7101, 2009.
• 2. WONG K., FONG T.-T., BIBBY, K., MOLINA M. Application of enteric viruses for faecal pollution source tracking in environmental waters. Environ Int. 45, 151, 2012.
• 3. LIN J., GANESH A. Water quality indicators: bacteria, coliphages, enteric viruses. Int. J. Environ. Health Res. 23, 484, 2013.
• 4. BRION G.M., MESCHKE J.S., SOBSEY M.D. F- specific coliphages: occurrence, types, and survival in natural waters. Water Res. 36, 2419, 2002.
• 5. FRANKE C., RECHENBURG A., BAUMANNS A., WILLKOMM M., CHRISTOFFELS E., EXNER M., KISTEMANN T. The emission potential of different land use patterns for the occurrence of coliphages in surface water. Int. J. Hyg. Environ. Health 212, 338, 2009.
• 6. GINO E., STAROSVETSKY J., ARON R. Bacteriophage ecology in a small community sewer system related to their role in sewage pollution in drinking water. Environ. Microbiol. 9, 2407, 2007.
• 7. GOURMELON M., CAPRAIS M.P., MIESZKIN S., MARTI R., WERY N., JARDE E., DERRIEN, M., JADASHECART A., COMMUNAL P.Y., JAFFREZIC A., POURCHER A. M. Development of microbial and chemical MST tools to identify the origin of the faecal pollution in bathing and shellfish harvesting waters in France. Water Res. 44, 4812, 2010.
• 8. OGORZALY L., TISSIER A., BERTRAND I., MAUL A. GANZER C. Relationship between F-specific RNA phage genogroups, faecal pollution indicators and human adenoviruses in river water. Water. Res. 43, 1257, 2009.
• 9. STEWART-PULLARO J., DAUGOMAH J.W., CHESTNUT D.E., GRAVE D.A., SOBSEY M.D., SCOTT G.I. F+RNA coliphage typing for microbial source tracking in surface waters. J. Appl. Microbiol. 101, 1015, 2006.
• 10. ARAUJO R.M., PUIG A., LASOBRAS J., LUCENA F., JOFRE J. Bacteriophages of enteric bacteria in fresh water with different levels of faecal pollution. J. Appl. Microbiol. 83, 627, 1997.
• 11. LEE J.E., LEE H., CHO Y.-H., HUR H.-G., KO G.P. F+ RNA coliphage –based microbial source tracking in water resources of South Korea. Sci. Total Environ. 412-413, 127, 2011.
• 12. HARAMOTO E., OTAGIRI M., MORITA H., KITAJAMA M. Genogroup distribution of F-specific coliphages in watstewater and river water in the Kofu basin in Japan. Lett. Appl. Microbiol. 54, 367, 2012.
• 13. MANDILARA G., MAVRIDOU A., VATAPOULUS A., RIGAS F. The use of bacteriophages for monitoring the microbiological quality of sewage sludge. Environ. Techol. 27, 367, 2006.
• 14. STEWART-PULLARO J., DAUGOMAH J.W., CHESTNUT D.E., GRAVE D.A., SOBSEY M.D., SCOTT G.I. F+RNA coliphage typing for microbial source tracking in surface waters. J. Appl. Microbiol. 101, 1015, 2006.
• 15. SUNDRAM A., JUMANLAL N., EHLERS M.M. Genotyping of F-RNA coliphages isolated from wastewater and river water samples. Water SA 32, 65, 2006.
• 16. KIRS M., SMITH D.C. Multiplex quantitative real-time reverse-transcriptase PCR for F+- specific RNA coliphages: a method for use in microbial source tracking. Appl. Environ. Microbiol. 73, 808, 2007.
• 17. JONES T.H., JOHNS M.W. Assessment of F-RNA coliphages as a potential indicator of enteric virus contamination of hog carcasses. J. Food Prot. 75, 1492, 2012.
• 18. PN-75/C-04615/05. Water and sludge. Microbiological analysis. Determination of coli group bacteria with the fermentation method. Warsaw, Poland.
• 19. PN-77/C-04615/07. Water and sludge. Microbiological analysis. Determination of faecal coli group bacteria with the fermentation method. Warsaw, Poland.
• 20. ANNON. Method 1602: Male specific (F+) and somatic coliphage in water by single agar layer procedure. United States of America Environmental Protection Agency; EPA 821-R-01-029, Washington, DC, 2001.
• 21. LONG S.C., SOBSEY M.D. A comparison of the survival of F+RNA and F+DNA coliphages in lake water microcosms. J. Water Health 2, 15, 2004.
• 22. KOTT Y., ROSE J., SPERBER S., BETZER N. Bacteriophages as viral pollution indicators. Water Res. 8, 165, 1974.
• 23. PUSCH D., OH D.-Y., WOLF S., DUMKE R., SCHROTER- BOBSIN U., HOHNE M., ROSKE I., SCHREIER E. Detection of enteric viruses and bacterial indicators in German environmental waters. Arch. Virol. 150, 929, 2005.
• 24. MUNIESA M., PAYAN A., MOCE-LLIVINIA L., BLANCH A.R., JOFRE J. Differential persistance of F-specific RNA phage subgropus hinders their use as single trackers for faecal source tracking in surface water. Water Res. 43, 1265, 2009.
• 25. JIANG S.C., CHU W., HE J.-W. Seasonal detection of human viruses and coliphages in Newport Bay, California. Appl. Environ. Microbiol. 73, 6468, 2007.
• 26. SKRABER S., GASSILOUD B., GANTZER C. Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water. Appl. Environ. Microbiol. 70, 3644, 2004.
• 27. MIERNIK A. Occurrence of bacteria and coli bacteriophages as potential indicators of faecal pollution of vistula River and Zegrze Reservoir. Pol. J. Environ. Stud. 13, 79, 2004.