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An analysis was carried out to determine the antibiotics evaluation of bacterial isolates associated with Imo State University (IMSU) Microbiology laboratory and sub offices. Swab sticks used for streaking were inoculated unto a freshly prepared nutrient agar, MacConkey agar for bacteria. Identification of bacteria was performed by determining their gram staining properties, biochemical reactions and sugar tests indicating their presence. The microbial isolates obtained from this study were identified to belong to bacteria genus of Klebsiella sp., Escherichia coli, Bacillus sp., Streptococcus sp., Staphylococcus sp., Shigella sp., Micrococcus sp., and Pseudomonas sp. Klebsiella sp. had the highest percentage of occurrence (39.3%) while Pseudomonas sp had the least occurrence (1.8%). Bacteria isolates showed the highest percentage of susceptibility to Tarivid (OFX) 21(25%) followed by Augmentin (AU) 13(15.5%), Gentamycin (CN) 10(11.9%), Nalidixic (NA) 9(10.7%), Ceporex (CEP) 7(8.3%), Septrin (SXT) 4(4.8%), Ciprofloxacin (CPX) 3(3.6%) while the lowest percentage of susceptibility was to Streptomycin (S) 1(1.2%). The effectiveness of decontamination ranges from high-level sterilization to simple cleaning with soap and water. Levels of decontamination include Sterilization, Disinfection, Antisepsis, and Cleaning. It is recommended that ethylene oxide gas is used in Sterilization of the air before any analysis is carried out.
Czasopismo
Rocznik
Tom
Strony
318-328
Opis fizyczny
Twórcy
autor
- Department of Microbiology, Faculty of Biological Sciences, Imo State University, Owerri, Nigeria
autor
- CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian 361021, China
autor
- Department of Chemistry, Faculty of Physical Sciences, Imo State University, Owerri, Nigeria
autor
- Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria
Bibliografia
- [1] Adams RI, Bateman, AC, Bik HM and Meadow JF. Microbiota of the indoor environment: a meta- analysis. Microbiome. 2015; 3, 1-18
- [2] Berryhill BA, Gil-Gil T, Manuel JA, Smith AP, Margollis E, Baquero F, Levin BR. What’s the Matter with MICs: bacterial Nutrition, limiting resources, and antibiotic pharmacodynamics. Microbiol Spectr 2023; 11(3), e04091-e4122. https://doi.org/10.1128/spectrum.04091-22
- [3] Kalwasifiska A, Burkowska A, Wilk I. Microbial air contamination in indoor environment of the university library. Ann Agric Environ. Med. 2012; 19(1), 25-34
- [4] Wamedo SA, Ede PN, Chuku A. Interaction Between Building Design and Indoor Airborne Microbial Load in Nigeria. Biol Sci. 2012; 5, 183-191
- [5] Delik E, Eroğlu B, Çolak ÇY, Özçelik AT, Öztürk BET. Alterations of growth, biofilm-forming, and gene expression of Bordetella pertussis by antibiotics at sub-minimal inhibitory concentrations. Res Microbiol 2023; 174(5), 104058. https://doi.org/10.1016/j.resmic.2023.104058
- [6] Chessbrough M. Medical laboratory manual for tropical countries. Cambridge University Press UK P2006. p. 508-511
- [7] Adams R.I, Bhangar S, Pasut W, Arens EA, Taylor JW, Lindow SE. Chamber bioaerosol study: outdoor air and human occupants as sources of indoor airborne microbes. Plos One 2014; 10: 23-29
- [8] Njoku NM. History of Owerri Municipal. String Publishers; 2001, p. 12-13
- [9] Bauer M, Mathieu L, Deloge-Abarkan M, Remen T, Tossa P and Hartemann P. Legionella bacteria in shower aerosols increase the risk of Pontiac fever among older people in retirement homes. J Epidemiol Commun 2008; 62, 913-920
- [10] Del L, Jaramillo M, Talledo M, Pons M, Flores L. Development of a 16S rRNA PCR-RFLP assay for Bartonella identification. Applicability in the identification of species involved in human infections. Universal J Microbiol 2014; 2(1): 15-22.
- [11] Ewing B, Hillier L, Wendl M, Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 2011; 8, 175-185
- [12] Tokuyasu H, Fukushima T, Nakazaki H, Shimuzu E. Infective endocarditis caused by Achromobacter xylosoxidans: a case report and review of the literature. Int Med. 2012; 51, 1133-1138
- [13] Poppert S, Essig A, Stoehr B, Steingruber A, Wirths B, Juretschko S, Reischl U, Wellinghausen, N. Rapid diagnosis of bacterial meningitis by realtime PCR and fluorescence in situ hybridization. J Clin Microbiol. 2005; 43(7), 3390-3397
- [14] Liberto MC, Lamberti AG, Marascio N, Matera G, Quirino A. Molecular identification of Bartonella quintana infection using species-specific real time PCR targeting transcriptional regulatory protein (bqtR) gene. J Mol Cell Probes, 2011; 25, 238-242
- [15] Curtis L, Lieberma A, Stark M, Rea W, Vetter M. Adverse health effects of indoor molds. J Nutr Envjjjiron Med. 2004; 14(3), 261-274
- [16] Abdulamir A, Al-baaj A, Al-Ramahy SK. Determination some virulence genes (avrxacE2, avrxacEl, hrpG, acrAB) in Pantoea spp. isolated from hospitals of Al-Diwaniyah city. Iraq. J. Phys. Conf. Ser. 2020, 1664(1), 012113. https://doi.org/10.1088/1742-6596/1664/1/012113
Typ dokumentu
article
Bibliografia
Identyfikatory
Identyfikator YADDA
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