Microbial and physiochemical quality of post harvest apples (Malus domestica Borkh) influenced by storage condition

• Autorzy: , , Ijeoma R. Okafor
• Języki publikacji: EN

Abstrakty

EN

This study assess the microbial and physiochemical quality of post-harvested apples (Malus domestica) under varying storage conditions, specifically ambient temperature and refrigeration temperature. Microbial analyses revealed distinct trends in bacterial and fungal counts. Standard microbiological and physiochemical techniques were adopted. For refrigerated apples, the total heterotrophic bacteria count peaked on Day 15 (1.8×105 CFU/g), contrasting with the lowest count on Day 3 (3.6×103 CFU/g). Similarly, ambient temperature of storage exhibited highest aerobic bacteria growth on Day 15 (3.3×104 CFU/g) and lowest on Day 2 (5.0×103 CFU/g). Staphylococcus counts in refrigerated apples peaked on Day 3 (3.6×103 CFU/g) and ambient temperature storage on Day 15 (7.4×103 CFU/g). The study identified seven bacterial genera, including Staphylococcus (19.6%), Bacillus (21.6%), and Proteus (17.6%) being prominent. Others were; 11.8% Micrococcus; 21.6% , Corynebacterium,; 13.7% Pseudomonas and 5.9% Citrobacter. Physiochemical attributes exhibited temperature-dependent variations. pH levels in refrigerated apples were lowest on Day 15 (2.88) and highest on Day 12 (3.80), whereas ambient temperature storage showed the highest pH (3.59) on Day 0 and lowest on Day 12 (3.42). Moisture content ranged from 80.2% (Day 3) to 87.8% (Day 15) for refrigerated apples, and from 85.7% (Day 1) to 87.8% (Day 15) for ambient temperature storage. Specific gravity decreased from 0.64 (Day 0) to 0.51 (Day 15) for refrigerated apples and from 0.64 (Day 0) to 0.59 (Day 15) for ambient temperature storage. Total suspended solids (TSS) fluctuated from 17.86% (Day 0) to 12.01% (Day 15) for ambient temperature and 12.54% (Day 15) for refrigerated temperature. The study recommends implementing optimized refrigeration protocols, considering the observed microbial growth patterns, could enhance the preservation of apples. While emphasizing stringent hygiene practices within storage facilities to be crucial, regular cleaning and disinfection routines even in refrigerators is a necessity.

Słowa kluczowe

EN

Ambient temperature; Post harvest apple; Refrigeration temperature microbial and physiochemical quality

• Wydawca: Scientific Publishing House „DARWIN”
• Czasopismo: World News of Natural Sciences
• Rocznik: 2024
• Tom: 57
• Strony: 146-160

Twórcy

autor

,

• Department of Microbiology, University of Port Harcourt, PMB 5323, Rivers State, Nigeria

autor

Ijeoma R. Okafor

• Department of Microbiology, University of Port Harcourt, PMB 5323, Rivers State, Nigeria

Bibliografia

• [1] Abadias, M., J. Usall, A. Anguera, C. Solsona and I. Vinas. Microbiological quality of fresh, minimally-processed fruit and vegetables and sprouts from retail establishments. Int. J. Food Microbiol. 123 (2008) 121-129
• [2] Ahmad, F., Zaidi, S., & Arshad, M. Postharvest quality assessment of apple during storage at ambient temperature. Heliyon, 7(8) (2021) 07714
• [3] Ali, S., Ganai, B. A., Kamili, A. N., Bhat, A. A., Mir, Z. A., Bhat, J. A., Tyagi, A., Islam, S. T., Mushtaq, M., Yadav, P., Rawat, S., & Grover, A. Pathogenesis-related proteins and peptides as promising tools for engineering plants with multiple stress tolerance. Microbiological Research, 212-213 (2008) 29-37
• [4] Aycicek, H., U. Oguz and K. Karci,. Determination of total aerobic and indicator bacteria on some raw eaten vegetables from whole sellers in Ankara, Turkey. Int. J. Hyg. Environ-Health, 209(2006) 197-201
• [5] Bagci, U. and A. Temiz. Microbiological Quality of fresh-squeezed orange juice and efficiency of fruit surface decontamination methods in microbiological quality. J. Food Protect. 74(8) (2011) 1238-1244
• [6] Badosa, E., R. Trias, D. Pares, M. Pla and E. Montesinos. Microbiological quality of fresh fruit and vegetable products in Catalinia (Spain) using normalized Plate-Counting methods and Real Time Polymerase Chain Reaction (QPCR). J. Sci. Food Agric. 88 (2008) 605-611
• [7] Bucker, A., A. Uba and T.I. Oyeyi. Occurrence of some enteropathegenic bacterial in some minimally and fully processed ready-to-eat foods in Kano metropolis, Nigeria. Afr. J. Food Sci. 4(2) (2010) 32-36
• [8] Cheesbrough, M. District Laboratory in Tropical Countries, Part 1. 2nd Edition, Cambridge University Press, Cambridge (2005).
• [9] De Giusti, M., C. Aurigemma, L. Marinelli, D. Tufi, D. De Medici, S. Di-Pasquale, C. De Vito and A. Boccia. The evaluation of the microbial safety of fresh ready-to-eat vegetables produced by different technologies in Italy. J. Appl. Microbiol. 109 (2010) 996-1006
• [10] EC-SCF. SCF/CS/FMH/SURF/Final, Risk profile on the Microbiological contamination of fruits and vegetables eaten raw. Report of the Scientific Committee on Food, European Commission Health and Consumer protection Directorate-General, 20 (2002)
• [11] Eni, A.O., I.A. Oluwawemitan and U.S. Oranusi,. Microbial quality of fruits and vegetables sold in Sango Ota, Nigeria. Afr. J. food Sci. 4(5) (2010) 291-296
• [12] Grabska, J., Beć, K. B., Ueno, N., & Huck, C. W. Analyzing the Quality Parameters of Apples by Spectroscopy from Vis/NIR to NIR Region: A Comprehensive Review. Foods (Basel, Switzerland), 12(10) (2023) 1946
• [13] ICMSf. Microbial Ecology of Food Commodities. Microorganisms in foods. Blackie Academic and Professional, London (1998)
• [14] Jha, S. N., Rai, D. R., & Shrama, R. Physico-chemical quality parameters and overall quality index of apple during storage. Journal of Food Science and Technology, 49(5) 9 (2012) 594–600
• [15] Joceyln, S., A. Reka, P. Leslie and Y. Hyun-Gyun. Microbiological quality of fresh vegetables and fruits sold in Singapore. Food Control 25 (2012) 39-44
• [16] Karanth, S., Feng, S., Patra, D., & Pradhan, A. K. Linking microbial contamination to food spoilage and food waste: the role of smart packaging, spoilage risk assessments, and date labeling. Frontiers in Microbiology 14 (2023) 1198124
• [17] Khan, B., Syed Z.A., Sikander S., Muhammad B., Jaffar A., Zobia J., Mohammad W., & Shagufta F. Postharvest quality of apple (Malus domestica Borkh) as influenced by storage conditions. International Journal of Advanced Research in Biological Sciences, 4(7) (2017) 154-163
• [18] Katherine, S. Catherine, M. and Rachel, F. (2006). Mycotoxins Explained. Food Safety & Hygiene, A Bulletin for the Australian Food Industry, November 2006.
• [19] Kopylov, V.I. Balykina, E.B. Berenstein, I.B. Burlak, V.A. Opanasenko, N.E. & Ryabov, V.A. Modern intensive fruit growing in the Crimea, 548 (Simferopol: PH “ARIAL”, 2017)
• [20] Mudgil, S., D. Argawal and A. Ganguli, (2004). Microbiological analysis of street Vended fresh squeezed carrot and Kinnow Mandarin juice in Patiala City. India. Internet J. Food Safety, 3: 1-3
• [21] Mazumdar, B. C. and K. Majumder. Methods onphysico-chemical analysis of fruits. Delhi: Daya Publishing House (2003)
• [22] Mahuya, M., M. Moumita and B. Pallabi,. Microbial contamination of Street vended Fruit Juices in Kolkata City. Internet J. Food Safety, 13 (2011) 1-5
• [23] Solomon, Oranusi & Braide (Dr), Wesley. Microbiological Safety Assessment of Apple Fruits (Malus domestica Borkh) Sold in Owerri Imo State Nigeria. Advance Journal of Food Science and Technology 4 (2012) 97-102
• [24] Oranusi, S., E. Onyeike, M. Galadima and V.J. Umoh, . Hazard analysis critical control points of foods prepared by families in Zaria, Nigeria. Nig. J. Microbiol. 18(1-2) (2004) 346-362
• [25] Oranusi, S., M. Galadima and V.J. Umoh,. Toxicity test and bacteriophage typing of S. aureus isolates from food contact surfaces and foods prepared by families in Zaria, Nigeria. Afr. J. Biotechnol. 5(4) (2006a) 362-365
• [26] Oranusi, S., M. Galadima and V.J. Umoh,. Phage typing and toxigenicity test of S. aureus strains from food contact surfaces and foods prepared in boarding schools in Zaria, Nigeria. Nig. J. Microbiol. 20(2) (2006b) 1011-1017
• [27] Oranusi, S., M. Galadima, V.J. Umoh and P.I. Nwanze. Food safety evaluation in boarding schools in Zaria, Nigeria, using the HACCP system. Scientific Research and Essay, 2(10) (2007) 426-433
• [28] Oranusi, S.U., V.J. Umoh and J.K.P. Kwaga. Hazards and critical control points of kunun-zaki, a non-alcoholic beverage in Northern Nigeria. Food Microbiol. 20 (2002) 127-132
• [29] Plugatar, Y.V. Sotnik, A.I. Denisova, O.A. Babina, R.D. Gorb, N.N. Comparative assessment of apple fruit storability during storage in different modes of the cooled medium. BIO Web of Conferences 39 (2021) 07002
• [30] Spengler, R.N. Origins of the apple: the role of megafaunal mutualism in the domestication of malus and rosaceous trees Front. Plant Sci. 10 (2019) 617
• [31] Tournas, V.H., . Moulds and Yeasts in fresh and minimally processed vegetables and sprouts. Int. J. Food Microbiol. 99 (2005)71-77
• [32] Ukwo, P.S., N.U. Ndaeyo and E.J. Udoh,. Microbiological quality and safety evaluation of fresh juices and edible ice sold in UYO metropolis, South Nigeria. Internet J. Food Safety, 13 (2011) 374-378
• [33] Uzeh, R.E., F.A. Alade and M. Bankole,. The microbial quality of pre-packed mixed vegetables salad in some retail outlets in Lagos, Nigeria. Afr. J. Food Sci. 3(9)(2009) 270-272
• [34] Vieira F. Borges, G.Copetti, C, Amboni, R. Denardi, F. Fett, R. Physico-chemical and antioxidant properties of six apple cultivars (Malus domestica Borkh) grown in southern Brazil. Sci. Hortic. (Amsterdam), 122 (2009) 421-425
• [35] Viswanathan, P. and R. Kaur,. Prevalence and growth of pathogens on salad vegetables, fruits and sprouts. Int. J. Hyg. Environ. Health, 203(2001) 105-213
• [36] Wani, N.R., Rather, R.A., Farooq, A. et al. New insights in food security and environmental sustainability through waste food management. Environ Sci Pollut Res 31, 17835–17857 (2024). https://doi.org/10.1007/s11356-023-26462-y
• [37] Yousuf, A., Musa, R., Isa, M. L. M., & Arifin, S. R. M.. Anxiety and Depression Among Women Living with HIV: Prevalence and Correlations. Clinical Practice and Epidemiology in Mental Health 16, (2020) 59-66

• Typ dokumentu: article